Evaluation and Comparison of Stability and Reliability of CBC Parameters Determined by Using Automatic Celltac G MEK-9100 Hematology Analyzer during Extended Storage at 4°C

Hussain, Sajid; Mehmood, Rubaida; Arshad, Farhatul Ain; Khan, Saqib

doi:10.4172/2155-9627.1000324

Cited by 3 publications

(5 citation statements)

References 20 publications

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“…When blood is stored at < 2 0 C, there is freezing injury to RBCs which causes hemolysis and if it is stored at > 6 0 C, overgrowth of unspecified bacteria is seen. [4][5][6] Fig. 1: Leishman stain (100 x Cytoplasmic and nuclear vacuole formation in WBCs In this study, blood samples stored in monitored refrigeration at 4 0 C for 24hours duration show improved stability in most of blood component parameters 3 and stability of WBC is also increased.…”

Section: Discussionmentioning

confidence: 55%

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Effect of room temperature and refrigerated storage on automated hematological parameters

D¹,

J²,

Eswari³

2020

IJPO

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For evaluating and monitoring of both inpatients and outpatients, complete blood count is one of the commonly performed investigations but at times it is challenging to evaluate all the blood samples on the collected day due to various factors like manpower shortages, weekends, and a single cell counter. Inspite of all these conditions we need to provide accurate report to patients. Hematology results are often influenced by the time between blood sampling and measurement as well as the storage conditions during sample delivery. This is because the hematological elements have limited stability in EDTA-anticoagulated blood. The stability of hematological parameters has improved after the anticoagulated blood is refrigerated.The aim is to investigate the effect of room temperature and refrigerated storage on complete blood counts and peripheral blood smear on automated hematological parameters. Blood samples were collected from 100 patients. Hematological parameters like Hemoglobin, PCV, MCV, MCH, MCHC, Platelet count, Red blood cell and White blood cell count were analyzed in room temperature storage and refrigerated storage at 40c for 24hrs using automated hematology analyzer. Peripheral blood smear from samples stored at room temperature and at 40 C in refrigerators for 24hrs were also examined.The study revealed RBC count and Hemoglobin were unaffected by storage at room temperature and refrigerated storage. Whereas MCV showed significant increase with storage at room temperature and WBC showed decrease in count with storage at room temperature that is preserved by refrigeration.

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Section: Discussionmentioning

confidence: 55%

“…In order to give an accurate final report to the patient it is essential to know the influence of climatic conditions on the hematological parameters mainly in tropical areas. [3][4][5] Ideally, hematologic parameters should be analyzed immediately after collection. If any delay is predicted, refrigeration at 4 0 C is optimal.…”

Section: Discussionmentioning

confidence: 99%

Effect of room temperature and refrigerated storage on automated hematological parameters

D¹,

J²,

Eswari³

2020

IJPO

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“…However, storage at > 6 • C lead to overgrowth of unspecified bacteria during sampling of blood. 1,14…”

Section: Discussionmentioning

confidence: 99%

“…Hematological reporting is very important aspect in accurate diagnosis & investigations of various organic disease, parasitic manifestations & metabolic disorders as well. 1 It includes complete blood count & peripheral smear which provides a definitive information to physicians or surgeons to diagnose, monitor & further management of patients. 2 Complete hemogram is also necessary for patients who requires blood transfusion to estimate the quantity of blood to be transfused.…”

Section: Introductionmentioning

confidence: 99%

“…4 In addition, many countries has been developed authorized central laboratories which receives blood samples from peripheral areas or other local laboratories which causes prolonged time of transportation & may lead to delayed evaluation of that particular hematological test.Hence, preanalytical phase needs a careful attention on procedures such as collection of blood samples, patient's identification, preference of collection vials, labelling of specimens, accurate blood suction, clerical errors & storage. 1 There is simple, modest method to maintain all hematological parameters & morphology of peripheral smear is storage of blood samples at 4 • C refrigeration. 7 Therefore, this study presents a quantitative data and peripheral blood smear changes for blood sample storage at fixed temperature 4 • C refrigeration for 24 hrs.…”

Section: Introductionmentioning

confidence: 99%

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Study of refrigerated storage of blood at 4°C on automated hematological parameters & morphological changes in peripheral blood smear: A prospective study

Kadam

Patil²,

Mane³

2023

IJPO

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: Complete hemogram & peripheral blood smear are primary tests done in day to day practice for correct diagnosis & further treatment of patients. EDTA blood samples are used for this regular screening tests which shows less stability in case of delaying sampling. Hence, the final results of these tests can be affected by different storage conditions & duration. This present study provides data analyzed from EDTA blood samples stored upto 24 hour at both room temperature & 4°C refrigeration.This study includes total 150 blood samples from indoor & outdoor patient without any specific criteria which were collected randomly. These blood samples were analyzed using haematological analyzer for complete blood count & their peripheral smear after storage in both room temperature & 4°C refrigeration for 24 hrs. There was significant increase in MCV & decrease in MCHC, reduced WBC count & platelet count with storage at room temperature which was prohibited by refrigeration. However, both room temperature & refrigerated storage does not affect RBC count & hemoglobin.Blood samples stored at room temperature for 24 hrs results in changes in haematological parameters & morphology of cells. Hence, refrigerated storage at 4°C is recommended for accurate results in case evaluation of delayed samples.

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Hematology and coagulation preanalytics for clinical chemists: Factors intrinsic to the sample and extrinsic to the patient

Winter

Pittman

Harris

2023

Clinical Biochemistry

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Evaluation and Comparison of Stability and Reliability of CBC Parameters Determined by Using Automatic Celltac G MEK-9100 Hematology Analyzer during Extended Storage at 4°C

Cited by 3 publications

References 20 publications

Effect of room temperature and refrigerated storage on automated hematological parameters

Effect of room temperature and refrigerated storage on automated hematological parameters

Study of refrigerated storage of blood at 4°C on automated hematological parameters & morphological changes in peripheral blood smear: A prospective study

Hematology and coagulation preanalytics for clinical chemists: Factors intrinsic to the sample and extrinsic to the patient

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