Evaluation and Determinants of Secondary Metabolites and its Antioxidant Activities of Various Fractions from Albizia myriophylla Bark

Abstract

Albizia myriophylla (ABZ) is a plant used in Thailand and Peninsular Malaysia for the treatment of diabetes mellitus type II. The antioxidant activities of ABZ have been suggested as one of the mechanisms for the observed beneficial effects. Hence, this study examined the phenolic, flavonoid, and saponin contents and antioxidant activity from methanol extract (ME) and its derived fractions hexane (HE), chloroform (CE), ethyl acetate (EAE), butanol (BE), and aqueous fraction of the bark of ABZ (AE). Amongst the extracts, EAE showed the highest total phenolic content of about 0.77 mg of gallic acid equivalent/g of extract (mg GAE/mg). However, the highest flavonoid content was detected in HE at 1.04 µg retinol equivalent ((RE)/g extract), while the saponin content was highest in CE at 1.1 µg diosgenin equivalent ((DE)/g extract). In 1,1-diphenyl-2-picrylhidrazyl (DPPH) radical scavenging test, EAE at 100 µg/mL had the highest percentage of inhibition of about 72.55%. 2-2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS radical scavenging test) showed BE had the highest percentage of inhibition at 100 µg/mL at 82.91%. Ferric reducing antioxidant power elucidated BE as having the highest percentage of inhibition which was 86.04% followed closely by ME at 85.90%. Thus, the different extracts of ABZ displayed various antioxidant capacity with probable free radical scavenging activity, which may be useful for the treatment of chronic inflammatory related metabolic diseases, such as diabetes and obesity.

Nutritional properties, determination of phenolic compounds and antioxidant potential of Victoria amazonica (Poepp.) J.C. Sowerby petiole

“…The results are shown in Table 2. For the total phenolic compound content, a signi cant difference between the samples is highlighted, and the ethyl acetate fraction derived from the methanolic extract showed the highest TPC, as also presented by [29] who observed a higher TPC in the ethyl acetate fraction of Albizia myriophylla bark methanolic extract. Based on the present data, it is possible to observe a relationship between TPC and antioxidant potential.…”

Chemotaxonomy in the Etlingera Genus from Sulawesi, Indonesia: Design and molecular docking of antioxidant marker

“…The maceration method allows contact between the sample and the solvent for a long time to maximize the transfer of compounds from the sample into the extraction solvent. In addition, the methanol solvent used is capable of extracting many classes of secondary metabolites such as anthocyanins, tannins, totarols, terpenoids, saponins, xanthoxyllines, quassinoids, phenones, polyphenols, lactones and flavones (Fonmboh et al 2020;Meng et al 2020). Mostly, methanol is used to extract various polar compounds, although there are some nonpolar groups that are quite soluble in this solvent.…”

Phytochemical Screening, In vitro Antioxidant Activities and Zebrafish Embryotoxicity of Abelmoschus esculentus Extracts

“…The amount of antioxidants found in these extracts is responsible to determine the DPPH radical scavenging activity. 26 Another important measure of antioxidant activity is the extract's reducing capacity. The presence of antioxidants in the sample would enable Fe 3+ to be reduced to Fe 2+ by donating an electron in the reducing power assay.…”

“…The residue was air-dried and further extracted with chloroform (CE) and methanol (ME) similar to the procedure carried out for the hexane extract. 26 The extraction of aqueous was performed on the residue by adding distilled water at a solid to solvent ratio of 1:30 (w/v) per extraction. The mixture was then left for 24 hours at 2°C.…”