Evaluation and HPLC characterisation of a new herbal ointment for the treatment of full-thickness burns in rats

Abstract

Objective Burn injuries are among the most common accidental health problems worldwide, frequently leading to health and socio-economic challenges. Despite this, no standard protocol for managing burn injuries can overcome the adverse effects of currently used drugs. The present study sets out to develop and evaluate the efficacy of new herbal ointments in providing synergistic anti-inflammatory, antimicrobial, antioxidant, and cell-proliferating activities. It also investigates the high-performance liquid chromatography (HPLC) characterisation of these new herbal ointments. Method Three different concentrations of the new herbal ointment, which incorporates extracts of Matricaria aurea flower heads, arial parts of Calendula tripterocarpa , Rosmarinus officinalis leaves, Alkanna tinctoria roots, and myrrh were developed and evaluated. Ointments designed to promote burn-wound healing were prepared and compared with β-sitosterol ointment and silver sulfadiazine cream, as a commercial standards. Results According to statistical and histopathological analyses and visual inspections, the new herbal formulas showed faster wound healing, more tolerability, and less toxicity than the commercial standards. Conclusion The new herbal ointments, developed in our study, have shown promising results. The formula offers mechanical protection without any release of non-biodegradable particles. It maintains the optimum moisture and pH of the skin, while minimising scar-tissue formation. These advantages, in addition to availability, low costs, and easy handling, may support the use of this new herbal formula as an effective and safe alternative treatment, designed to promote the healing of burn injuries.

Rosmarinus officinalis and Skin: Antioxidant Activity and Possible Therapeutical Role in Cutaneous Diseases

“…In the past decade, a new and interesting strand of phytotherapeutic research has become increasingly popular, which aims to evaluate the curative potential of Rosmarinus officinalis in wound healing, as well as in promoting the survival of skin flaps. Concerning the wound-healing process, beyond some in vivo evidence of the regenerative potential of Rosmarinus officinalis in the treatment of acute wounds, especially burn wounds, much of the scientific evidence instead concerns the therapeutic potential of rosemary in chronic wounds, primarily diabetic wounds [ 82 , 83 ].…”

Exploring Iberian Peninsula Lamiaceae as Potential Therapeutic Approaches in Wound Healing

“…The healing effect of rosemary on burn wounds has also been tested by other authors [ 77 , 83 , 108 , 109 ]. Regarding the in vivo thermal-induced wounds, the activity of two different ointments prepared with S. rosmarinus and Populus alba L. essential oils was studied.…”

Optimization of PEG 400 and PEG 6000 in Simplicia Ointment of Laportea decumana (Roxb.) Wedd

“…Organoleptic Test An initial step in assessing a formulation's quality is to conduct organoleptic testing on an ointment. By contrasting it with three formulations, the quality of this ointment is determined (Farhan et al, 2021). Table 2 displayed the results of the organoleptic test.…”