Evaluation and quantification of spectral information in tissue by confocal microscopy

Maeder, Ulf; Marquardt, Kay; Beer, Sebastian; Bergmann, Thorsten; Schmidts, Thomas; Heverhagen, Johannes T.; Zink, Klemens; Runkel, Frank; Fiebich, Martin

doi:10.1117/1.jbo.17.10.106011

Cited by 11 publications

(7 citation statements)

References 43 publications

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“…An HSI system has been combined with many other techniques, such as laparoscope, 46 colposcope, 34 fundus camera, 76,113,114 and Raman scattering, 115 in order to leverage the key benefits of each instrument individually and provide more useful information for disease diagnosis and treatment. The most common combination is with microscope 35,38,85,[116][117][118][119][120][121] or confocal microscope, 122 which has been proved useful in the investigation of the spectral properties of tissue.…”

Section: Combination With Other Techniquesmentioning

confidence: 99%

Medical hyperspectral imaging: a review

2014

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Hyperspectral imaging (HSI) is an emerging imaging modality for medical applications, especially in disease diagnosis and image-guided surgery. HSI acquires a three-dimensional dataset called hypercube, with two spatial dimensions and one spectral dimension. Spatially resolved spectral imaging obtained by HSI provides diagnostic information about the tissue physiology, morphology, and composition. This review paper presents an overview of the literature on medical hyperspectral imaging technology and its applications. The aim of the survey is threefold: an introduction for those new to the field, an overview for those working in the field, and a reference for those searching for literature on a specific application.

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Section: Combination With Other Techniquesmentioning

confidence: 99%

Medical hyperspectral imaging: a review

2014

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“…220 In addition, the spectral confocal microscopes have also been used for fluorescence evaluation and quantification in different tissues, such as brain slices, mouse ovaries, skin sections, kidney slice, and breast cancer tissues. 134,221,222 Neher et al demonstrated that quantitative FRET can be performed by taking advantage of steady-state intensity measurements, but it only allows determination of the apparent FRET efficiency, the relative concentration of donor, and the concentration of the acceptor. 223 They also developed a nonnegative matrix factorization (NMF) algorithm to detect and separate spectrally distinct components of multiple labeled fluorescence images.…”

Section: Fluorescence and Cytogeneticsmentioning

confidence: 99%

“…237 Maeder et al introduced a confocal laser-scanning microscope equipped with a detection unit that provides high spectral resolution method to visualize and evaluate the spatial distribution of spectral information in skin sections. 221 In a more recent study by Nallala et al, an IR spectral imaging system was used for histopathological recognition in colon cancer diagnosis. 238 Fourier transform IR spectral imaging system has also been used to perform the histopathology mapping of biochemical changes in myocardial infarction, live epithelial cancer cells, histologic and biochemical correlations in tissue engineered cartilage, and histomorphological evaluation of oral squamous cell carcinoma of the oral cavity.…”

Section: Histological Analysismentioning

confidence: 99%

Review of spectral imaging technology in biomedical engineering: achievements and challenges

et al. 2013

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Spectral imaging is a technology that integrates conventional imaging and spectroscopy to get both spatial and spectral information from an object. Although this technology was originally developed for remote sensing, it has been extended to the biomedical engineering field as a powerful analytical tool for biological and biomedical research. This review introduces the basics of spectral imaging, imaging methods, current equipment, and recent advances in biomedical applications. The performance and analytical capabilities of spectral imaging systems for biological and biomedical imaging are discussed. In particular, the current achievements and limitations of this technology in biomedical engineering are presented. The benefits and development trends of biomedical spectral imaging are highlighted to provide the reader with an insight into the current technological advances and its potential for biomedical research.

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“…Since the vital nerve structures in the prostate are embedded in a fibrofatty plate in the pelvic region 47 , 51 , a dual fluorophore staining protocol using Oxazine 4 to highlight nerves and Nile Red to delineate adipose was also developed to mitigate non-specific adipose fluorescence, which would diminish nerve SBR. Nile Red is a fluorogenic, lipid-specific oxazine fluorophore that is spectrally distinct from Oxazine 4 52 - 56 , facilitating separation of nerve and adipose tissue fluorescence using a two-color imaging strategy. Translation of the optimized direct administration methodology to radical nerve-sparing prostatectomy could significantly improve cavernous nerve visualization and preservation, decreasing patient morbidity and improving post-surgical quality of life for prostate cancer survivors.…”

Section: Introductionmentioning

confidence: 99%

Direct Administration of Nerve-Specific Contrast to Improve Nerve Sparing Radical Prostatectomy

Barth

Gibbs

2017

Theranostics

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Nerve damage remains a major morbidity following nerve sparing radical prostatectomy, significantly affecting quality of life post-surgery. Nerve-specific fluorescence guided surgery offers a potential solution by enhancing nerve visualization intraoperatively. However, the prostate is highly innervated and only the cavernous nerve structures require preservation to maintain continence and potency. Systemic administration of a nerve-specific fluorophore would lower nerve signal to background ratio (SBR) in vital nerve structures, making them difficult to distinguish from all nervous tissue in the pelvic region. A direct administration methodology to enable selective nerve highlighting for enhanced nerve SBR in a specific nerve structure has been developed herein. The direct administration methodology demonstrated equivalent nerve-specific contrast to systemic administration at optimal exposure times. However, the direct administration methodology provided a brighter fluorescent nerve signal, facilitating nerve-specific fluorescence imaging at video rate, which was not possible following systemic administration. Additionally, the direct administration methodology required a significantly lower fluorophore dose than systemic administration, that when scaled to a human dose falls within the microdosing range. Furthermore, a dual fluorophore tissue staining method was developed that alleviates fluorescence background signal from adipose tissue accumulation using a spectrally distinct adipose tissue specific fluorophore. These results validate the use of the direct administration methodology for specific nerve visualization with fluorescence image-guided surgery, which would improve vital nerve structure identification and visualization during nerve sparing radical prostatectomy.

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Evaluation and quantification of spectral information in tissue by confocal microscopy

Cited by 11 publications

References 43 publications

Medical hyperspectral imaging: a review

Medical hyperspectral imaging: a review

Review of spectral imaging technology in biomedical engineering: achievements and challenges

Direct Administration of Nerve-Specific Contrast to Improve Nerve Sparing Radical Prostatectomy

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