Evaluation and verification of the characteristic peptides for detection of Staphylococcus aureus in food by targeted LC-MS/MS

Zhao, Hong; Wang, Yuying; Zhao, Lei; Dong, Zhizheng; Mi, Jiebo; Zhao, Huabing; Wang, Jing; Zeng, Jing; Wang, Hongbin; Wang, Libing

doi:10.1016/j.talanta.2021.122794

Cited by 6 publications

(3 citation statements)

References 25 publications

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“…In addition, Staphylococcus aureus is an opportunistic pathogen capable of causing a wide variety of infections, ranging from skin infections to severe invasive infections and even necrotizing pneumonia and sepsis [ 59 , 60 , 61 ]. Routine culture remains an accurate way to detect S. aureus , but it is time-consuming.…”

Section: Applications In Diagnosismentioning

confidence: 99%

A Promising Tool in Serological Diagnosis: Current Research Progress of Antigenic Epitopes in Infectious Diseases

et al. 2022

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Infectious diseases, caused by various pathogens in the clinic, threaten the safety of human life, are harmful to physical and mental health, and also increase economic burdens on society. Infections are a complex mechanism of interaction between pathogenic microorganisms and their host. Identification of the causative agent of the infection is vital for the diagnosis and treatment of diseases. Etiological laboratory diagnostic tests are therefore essential to identify pathogens. However, due to its rapidity and automation, the serological diagnostic test is among the methods of great significance for the diagnosis of infections with the basis of detecting antigens or antibodies in body fluids clinically. Epitopes, as a special chemical group that determines the specificity of antigens and the basic unit of inducing immune responses, play an important role in the study of immune responses. Identifying the epitopes of a pathogen may contribute to the development of a vaccine to prevent disease, the diagnosis of the corresponding disease, and the determination of different stages of the disease. Moreover, both the preparation of neutralizing antibodies based on useful epitopes and the assembly of several associated epitopes can be used in the treatment of disease. Epitopes can be divided into B cell epitopes and T cell epitopes; B cell epitopes stimulate the body to produce antibodies and are therefore commonly used as targets for the design of serological diagnostic experiments. Meanwhile, epitopes can fall into two possible categories: linear and conformational. This article reviews the role of B cell epitopes in the clinical diagnosis of infectious diseases.

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Section: Applications In Diagnosismentioning

confidence: 99%

A Promising Tool in Serological Diagnosis: Current Research Progress of Antigenic Epitopes in Infectious Diseases

et al. 2022

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“…At present, researchers have developed many analytical methods for detecting SEC, including the polymerase chain reaction (PCR), 8,9 liquid chromatography−mass spectrometry (LC−MS), 10,11 fluorometric assay, 12,13 and colorimetric method. 14,15 However, each of these techniques has its own drawbacks.…”

Section: ■ Introductionmentioning

confidence: 99%

“…At present, researchers have developed many analytical methods for detecting SEC, including the polymerase chain reaction (PCR), , liquid chromatography–mass spectrometry (LC–MS), , fluorometric assay, , and colorimetric method. , However, each of these techniques has its own drawbacks. The limitations of the PCR and LC–MS are high cost and complicated operation, and the detection signal of the fluorometric assay is easily disturbed by humidity and oxygen in the environment. , The main disadvantage of the colorimetric method is low sensitivity .…”

Section: Introductionmentioning

confidence: 99%

Tunable Preparation of SERS-Active Au–Ag Janus@Au NPs for Label-Free Staphylococcal Enterotoxin C Detection

Jin

Zhao

2023

J. Agric. Food Chem.

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Trace staphylococcal enterotoxin C (SEC) in food poses a serious risk to human health, and it is vital to develop a sensitive and accurate approach for SEC monitoring. Herein, a surface-enhanced Raman scattering (SERS) aptasensor was developed for the quantitative detection of SEC. SERS-active gold–silver Janus@gold nanoparticles (Au–Ag Janus@Au NPs) were prepared and showed tunable solid and hollow nanostructures by simply controlling the pH values of the reaction system. Solid Au–Ag Janus@Au NPs exhibited intrinsic and enhanced SERS activity due to the intense plasmonic coupling effect between Au dots and Au–Ag Janus NPs, which was 2.27-fold and 17.46-fold higher than that of Au–Ag Janus NPs and hollow Au–Ag Janus@Au NPs, respectively. The attachment of multiple Au dots also protected Ag islands from oxidization, which increased the stability of Au–Ag Janus@Au NPs. Solid Au–Ag Janus@Au NPs served as a label-free, strong, and stable SERS detection probe and achieved sensitive and reliable detection of SEC. The limit of detection was as low as 0.55 pg/mL. This study will expand the application prospects of label-free SERS detection probes in complex systems for food safety monitoring.

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Characterization of the natural peptidome of four leeches by integrated proteogenomics and pseudotargeted peptidomics

et al. 2023

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Evaluation and verification of the characteristic peptides for detection of Staphylococcus aureus in food by targeted LC-MS/MS

Cited by 6 publications

References 25 publications

A Promising Tool in Serological Diagnosis: Current Research Progress of Antigenic Epitopes in Infectious Diseases

A Promising Tool in Serological Diagnosis: Current Research Progress of Antigenic Epitopes in Infectious Diseases

Tunable Preparation of SERS-Active Au–Ag Janus@Au NPs for Label-Free Staphylococcal Enterotoxin C Detection

Characterization of the natural peptidome of four leeches by integrated proteogenomics and pseudotargeted peptidomics

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