Évaluation de sept laveurs-désinfecteurs d'endoscopes : activité bactéricide des désinfectants, efficacité antibactérienne des couples laveur-désinfecteur/produits

Efficacy of 10 different cleaning processes in a washer–disinfector for flexible endoscopes

“…Such study yielded differences in the achieved processing result. 17 Using a test model, we carried out the first study in which the cleaning stage of a WD was evaluated alone. 18 Our results showed that cleaning processes differ in their ability to remove blood residues and reduce bioburden.…”

Susceptibility patterns of bacteria isolated from the hospital environment towards disinfectants commonly used for surfaces and medical devices

“…The susceptibility profile of strains to disinfectants is estimated by "bacterial growth (+)" or "non-growth (-)" compared to the two controls: positive fertility control and negative sterility control for each [16]. The plate technique (microdilution) used has the advantage of studying all the strains simultaneously with various disinfectants' dilutions [16,24], in contrast to European standards [33,34]. According to Russel (2003), a biocide was resistant if it does not show an antibacterial effect at the concentration recommended by the manufacturer [35].…”

“…The susceptibility of bacterial strains against disinfectants is carried out by the micro method described by Rouillon et al (2006) [24]. This method involves the incubation of microbial strains with serial dilutions of the evaluated disinfectant.…”

“…Techniques for the assessment of disinfectants against bacteria are standardized (French standard 1276 March 2010). However, the micro method is a preliminary evaluation, more practical, faster, and reliable [16,24].…”

“…Sixty μL of this culture were added to 2 mL of BHI and incubated at 37 ° C for 2 hours to obtain a culture in the exponential growth phase. Two hundred microliters of this culture were added to 1.8 ml of BHI at 37 ° C to get the final suspension [24].…”