Evaluation in Drosophila melanogaster of the mutagenic potential of furfural in the mei-9a test for chromosome loss in germ-line cells and the wing spot test for mutational activity in somatic cells

Nonlethal Furfural Exposure Causes Genomic Alterations and Adaptability Evolution in Saccharomyces cerevisiae

“…The rates of aneuploidy, chromosomal rearrangements, and LOH in these isolates were 50-, 23-, and 4-fold higher, respectively, than those in untreated cells ( Table 1 ). Our findings are consistent with the teratogenic and recombinogenic effects observed in fly and human cell cultures ( 11 , 34 ). Remarkably, 0.6 g/L furfural had a stronger induction effect on aneuploidy than LOH ( Table 1 ).…”

An Assay to DetectIn VivoY Chromosome Loss inDrosophilaWing Disc Cells

“…Drosophila melanogaster is an appropriate model species for the analysis of numerous basic biologic processes, including mutagenesis (Bellen et al 2010). There have been a number of Drosophila -based aneuploidy test procedures developed to detect gain and/or loss of chromosomes both in the germline and in the soma (Szabad 1986; Szabad and Wurgler 1987; Rodriguez-Arnaiz et al 1992; Szabad et al 1995). However, most of these techniques detect aneuploidy in the female and/or in the male germ line, and there are two major difficulties associated with the germ-line based procedures: (1) a limited number of the germline cells and (2) the long time course between the induction and the detection of the aneuploidy (Szabad and Bennettova 1986; Szabad et al 1995).…”

Furfural [MAK Value Documentation, 1998]

“…Furfural yielded positive results in a wing spot test with D. melanogaster (Rodriguez-Arnaiz et al 1992). In another study with D. melanogaster (sex-linked recessive lethal test and translocation test; Woodruff et al 1985) the dose used was clearly too low, as mortality was rarely observed.…”