Evaluation in Mouse Model of Combined Virus-bacterial Vaccine Based on Attenuated Influenza A(H7N3) Virus and the Group B Streptococcus Recombinant Polypeptides

Abstract

Background:Secondary bacterial influenza complications are a common cause of excesses morbidity and mortality, which determines the need to develop means for specific prophylaxis. Group B streptococcal infection is especially common cause of pneumonia among children and the elderly with underlying conditions. Here we investigate in a mouse model the effects of combined intranasal immunization using live attenuated influenza vaccine and recombinant polypeptides based on group B Streptococcus surface proteins.Methods:Groups of outbred mice received two doses of the following preparations: 1) the reassortant A/17/Mallard/Netherlands/00/95 (H7N3) influenza virus; 2) a mixture of P6, ScaAB, ScpB1 and Stv recombinant GBS proteins (20 µg total); 3) the A(H7N3) influenza vaccine pooled with the four bacterial peptide preparation; 4) control animals were treated with PBS.Results:Intranasal vaccination using LAIV in combination with GBS polypeptides provided advantageous protection against infections with homologous A/Mallard/Netherlands/12/00 (H7N3) wild type virus or heterologous A/Puerto Rico/8/34 (H1N1) followed by serotype II GBS infection. Also, combined vaccination improved bacterial clearance from the lungs of mice.Conclusion:Intranasal immunization with LAIV+GBSV was safe and enabled to induce the antibody response to each of vaccine components. Thus, the combined vaccine increased the protective effect against influenza and its bacterial complications in mice compared to LAIV-only.

Factors of early protective action of live influenza vaccine combined with recombinant bacterial polypeptides against homologous and heterologous influenza infection

“…The titers of IgG antibodies to the viruses A/H7N3 and A/H7N9 but not to A/H1N1 significantly increased by the 21st day after immunization, compared with day 5 (P < 0.001). This data confirm the previously obtained results on the immunogenicity of an associated vaccine based on LAIV and recombinant GBS polypeptides [1, 2]. Fig.…”

“…Associated viral-bacterial intranasal immunization based on live influenza vaccines (LAIV) and recombinant bacterial polypeptides (either by simple mixing of preparations or by creating genetically engineered constructions) is a new direction of vaccine prophylaxis, which allows to reach the protective effect against post-influenza bacterial complications by one vaccination. This effect is achievable both due to the reduction of the primary viral infection, and through the formation of specific antibodies against bacterial polypeptides [1]. For the prevention of respiratory infections, it is important to develop mucosal vaccines to stimulate not only systemic but also local humoral immunity.…”

Prevention of Influenza A(H7N9) and Bacterial Infections in Mice Using Intranasal Immunization With Live Influenza Vaccine and the Group B Streptococcus Recombinant Polypeptides

“…In a previous study, we have shown that associated intranasal immunization of outbred mice using live attenuated influenza vaccine (LAIV) of H7N3 subtype and 4 GBS recombinant polypeptides was effective against pulmonary infection with five hundred 50% mouse infectious doses (MID 50 ) of avian A/mallard/ Netherlands/12/00(H7N3) or 100 MID 50 A/PR8/34(H1N1) influenza followed by GBS serotype II secondary infection. 13 In this study, we evaluated whether immunity generated against H7N3 LAIV and GBS polypeptides can protect against H7N9 influenza infection followed by GBS burden. We also made an attempt to reveal the role of some causes of innate and adaptive immunity in such protection.…”

Combined immunization with attenuated live influenza vaccine and chimeric pneumococcal recombinant protein improves the outcome of virus-bacterial infection in mice

“…Previously, we proved the effectiveness of mixed immunization against influenza and group B Streptococcus in alleviating the course of a virus-bacterial co-infection. A mixture of live influenza vaccine and several polypeptides, recombinant analogs of group B streptococcal surface proteins, was successfully used as a vaccine preventing the GBS infection [5,6]. In the present work we employed a mixture of live influenza vaccine and PSPF protein, a genetically engineered preparation against pneumococcal infection, for vaccination.…”