Evaluation of 11 microsatellite loci for their use in paternity testing in Yugoslav Pied cattle (YU Simmental cattle)

Abstract

Eleven microsatellite loci (TGLA227, BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA023, ETH3, ETH225, BM1824) were evaluated for their use in paternity testing in the Yugoslav Pied cattle (YU Simmental cattle) population in Serbia. A total of 40 animals were tested. At the 11 tested loci, a total of 91 alleles were detected. The mean number of alleles per locus was 8.273. Polymorphism information content (PIC) values ranged from 0.58 to 0.88 with the mean value of 0.72. The most informative loci were: TGLA53 (14 alleles, PIC = 0.88), TGLA227 (11 alleles, PIC = 0.82), INRA023 (11 alleles, PIC = 0.86), BM2113 (9 alleles, PIC = 0.80). Combined power of discrimination (CPD) for the 11 microsatellite loci was 0.999. The results of the present study confirm that the analysed set of 11 microsatellite markers recommended by ISAG is suitable for paternity testing in Yugoslav Pied cattle in Serbia.

Türkiye Yerli Sığır Irklarının Mikrosatellit Belirteçler ile Genetik Karakterizasyonu: Kimliklendirme Çalışmalarında Kullanılabilirliği

“…By using the same 7 [29] and 11 markers [3] in this study, PE values have been found to be 0.188-0.629 [29] and 0.175-0.552 [3] for Gry and Yugoslav Pied cattle, respectively. The total PE values were 0.979 [29] and 0.996 [3] . The total PE values were estimated for Holstein-Friesian, Brown Swiss ÖZŞENSOY, KURAR, DOĞAN, BULUT NİZAMLIOĞLU, IŞIK, ÇAMLIDAĞ, ALTUNOK and their crosses with native cattle breeds in Turkey [30] and found to be similar (>0.9999) with this study.…”

“…Comparing with other marker systems, microsatellites [1] , exist widely in the genome, have features of higher polymorphism and codominant inheritance [2] . Due to their high polymorphism and technical ease including suitability for PCR technology and capillary electrophoresis, microsatellites are widely preferred in the paternity testing efforts of various mammalian species [3] . Correct determination of genetic relationships among animal populations has critical importance for development of selection programs [3] , generation of pedigree structures [4] , estimation of heritability [5,6] , and breeding values [7] .…”

“…Due to their high polymorphism and technical ease including suitability for PCR technology and capillary electrophoresis, microsatellites are widely preferred in the paternity testing efforts of various mammalian species [3] . Correct determination of genetic relationships among animal populations has critical importance for development of selection programs [3] , generation of pedigree structures [4] , estimation of heritability [5,6] , and breeding values [7] . Significantly higher rates of paternity misidentification were reported even in the countries where herd records are performed with great care [3,5,8] .…”

“…Paternity misidentification was determined to cause serious deviations (5-50%) in the estimation of genetic parameters and reduction of genetic gain in selection programs [12] . It was reported that more than 20% of paternity misidentification cases were caused by artificial insemination of more than one bull [3,11] and it can be reduced to 8% by using a quality control system which results in an increase of 1% in genetic progress [11] . It is well known that herd improvement can be performed to have extra profits by accurate estimation of parent identification [9] .…”

Effectiveness of microsatellite and single nucleotide polymorphism markers for parentage analysis in European domestic pigs

“…Short tandem repeats (STRs, microsatellites) have been the recent and internationally preferred molecular markers to trace studbook information from the species down to the individual level in all types of organisms ranging from lianas (Lacombe et al, 2013) to fish (Liu et al, 2012), insects (Wong et al, 2012), birds (Hadfield et al, 2006;Guerier et al, 2012), and mammals (Cutullé et al, 2010;Stevanovic et al, 2010;Souza et al, 2012). Microsatellites with a high level of heterozygosity are relatively more powerful in assigning relatedness per locus than those with a low level of heterozygosity.…”