Evaluation of 3D Printing and Its Potential Impact on Biotechnology and the Chemical Sciences

Gross, Bethany C.; Erkal, Jayda L.; Lockwood, Sarah Y.; Chen, Chengpeng; Spence, Dana M.

doi:10.1021/ac403397r

Cited by 1,462 publications

(1,153 citation statements)

References 153 publications

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“…The paperbased iCVD substrate (1 × 1 cm) was soaked in 1, 1.5, and 3% (wt/vol) sodium alginate (FMC biopolymer), respectively. Samples at the desired time points (10,20,30, and 60 min) were collected and imaged using AM413MT Dino-Lite X (Dino-Lite). The hydrogel thickness was analyzed as three random points were chosen, and the average was calculated by DinoCapture 2.0 (Dino-Lite).…”

Section: Methodsmentioning

confidence: 99%

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Hydrogel-laden paper scaffold system for origami-based tissue engineering

Kim

Lee

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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In this study, we present a method for assembling biofunctionalized paper into a multiform structured scaffold system for reliable tissue regeneration using an origami-based approach. The surface of a paper was conformally modified with a poly(styrene-comaleic anhydride) layer via initiated chemical vapor deposition followed by the immobilization of poly-L-lysine (PLL) and deposition of Ca 2+ . This procedure ensures the formation of alginate hydrogel on the paper due to Ca 2+ diffusion. Furthermore, strong adhesion of the alginate hydrogel on the paper onto the paper substrate was achieved due to an electrostatic interaction between the alginate and PLL. The developed scaffold system was versatile and allowed area-selective cell seeding. Also, the hydrogel-laden paper could be folded freely into 3D tissue-like structures using a simple origamibased method. The cylindrically constructed paper scaffold system with chondrocytes was applied into a three-ring defect trachea in rabbits. The transplanted engineered tissues replaced the native trachea without stenosis after 4 wks. As for the custom-built scaffold system, the hydrogel-laden paper system will provide a robust and facile method for the formation of tissues mimicking native tissue constructs.paper scaffolds | origami | tissue engineering | initiated chemical vapor deposition | hydrogel T he living organ changes its shape from a sheet-like arrangement with primitive cells to mature three-dimensional (3D) structures through morphogenetic processes (1-3). To date, a wide range of biomaterials have been used for the total or partial replacement of damaged organs and/or tissue structures (4-7). As the functions of the living organ are realized by periodic changes in the spatial arrangement of tissue elements, multiform scaffold systems mimicking the native tissue are desired. Moldcasting and electrospinning, among various other methods, have been introduced to fabricate diverse scaffolds (8, 9). These fabrication processes, however, possess limitations for organlike structure productions. Although recent progress in tissue engineering has focused on using 3D printer schemes, there are still limitations such as the shortage of appropriate printing materials and technical challenges related to the sensitivity of living cells (10-12).Paper-based scaffolds have been used previously for cell culture platforms (13), high-throughput biochemical assay platforms (14), and a point-of-care diagnostic system (15). As a nature-originated substrate, paper has attracted enormous research interest for applications in tissue engineering (16). Cellulose-based paper may serve as a promising material for tissue engineering as it contains macroporous structures that allow nutrient transport and oxygenation (13). In this regard, paper origami is a simple alternative approach for fabricating a multiform scaffold. Based on computeraided design (CAD) planar figures, a variety of shaped scaffolds could be designed using biofunctionalized paper.In this report, a vapor-phase method, init...

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Section: Methodsmentioning

confidence: 99%

“…These fabrication processes, however, possess limitations for organlike structure productions. Although recent progress in tissue engineering has focused on using 3D printer schemes, there are still limitations such as the shortage of appropriate printing materials and technical challenges related to the sensitivity of living cells (10)(11)(12).…”

mentioning

confidence: 99%

Hydrogel-laden paper scaffold system for origami-based tissue engineering

Kim

Lee

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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“…Recent reviews describe state of the art 3D-printing for microfluidics applications. 18,19 All the demonstrations of 3D-printed microfluidics so far employ active flow control (usually pneumatic or centrifugal pumps). The resolution currently available with consumer grade 3D-printers is typically ≥ 200 µm 20,21 with ~ 1 µm surface roughness.…”

Section: D-printed Microfluidicsmentioning

confidence: 99%

3D-Printed Autonomous Capillaric Circuits^†

Olanrewaju

Robillard

Dagher

et al. 2016

Preprint

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Capillaric circuits (CCs) are advanced capillary microfluidic devices that move liquids in complex pre-programmed sequences without external pumps and valves -relying instead on microfluidic control elements powered by capillary forces. CCs were thought to require high-precision micro-scale features manufactured by photolithography in a cleanroom, which is slow and expensive. Here we present rapidly and inexpensively 3D-printed autonomous CCs. Molds for CCs were fabricated with a benchtop 3D-printer, Poly(dimethylsiloxane) replicas were made, and fluidic functionality was verified with aqueous solutions. We established design rules for 3D-printed CCs by a combination of modelling and experimentation. The functionality and reliability of 3D-printed trigger valves -an essential fluidic element that stops one liquid until flow is triggered by a second liquid -was tested for different geometries and different solutions. Trigger valves with geometries up to 80-fold larger than cleanroom-fabricated ones were found to function reliably. We designed 3D-printed retention burst valves that encode sequential liquid drainage and delivery using capillary pressure differences encoded by varying valve height and width. Using an electrical circuit analogue of the CC, we established circuit design rules for ensuring strictly sequential liquid delivery. We realized a 3D-printed CC with reservoir volumes 60 times larger than cleanroom-fabricated circuits and autonomously delivered eight liquids in a pre-determined sequence in < 7 min, exceeding the number of sequentially-encoded, self-regulated fluidic delivery events previously reported. Taken together, our results demonstrate that 3D-printing enables rapid prototyping of reliable CCs with improved functionality and potential applications in diagnostics, research and education.

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“…Microfluidic devices are used in fields such as bioanalysis, materials engineering, and chemistry (Femmer et al 2015;Gross et al 2014;Shah et al 2008;Shirk et al 2013;Utada et al 2007;Ye et al 2015). These devices are especially useful in bioanalysis because they have the ability to manipulate relatively small volumes of fluid, requiring small samples from a test subject.…”

Section: Introductionmentioning

confidence: 99%

“…3D-printed templates also allow for accelerated testing and modification of device designs; modifications can be added to the template file and printed out immediately (Gross et al 2014;O'Neill et al 2014). MacDonald, et al (2002) have demonstrated a method to create microfluidic devices in PDMS using solid object printing.…”

Section: Introductionmentioning

confidence: 99%

Developing a Protocol for Creating Microfluidic Devices with a 3D Printer

Collette

Novak

Rosen

et al. 2017

IJURCA

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This work has undergone a double-blind review by a minimum of two faculty members from institutions of higher learning from around the world. The faculty reviewers have expertise in disciplines closely related to those represented by this work. If possible, the work was also reviewed by undergraduates in collaboration with the faculty reviewers. AbstractMicrofluidics devices have high importance in fields such as bioanalysis because these devices have the ability to manipulate small volumes of fluid, typically ranging from microliters to picoliters. Small samples of fluids can be quickly and easily tested using reactions performed with complex microfluidic devices. Many methods have been previously developed to create these devices, including traditional nano-lithography techniques borrowed from the field of microelectronics. However, these traditional techniques are cost-prohibitive for many small-scale laboratories. This research explores a relatively low-cost technique using a 3D printed master, which is used as a template for the fabrication of polydimethylsiloxane (PDMS) microfluidic devices. The masters are designed using computer aided design (CAD) software and can be printed and modified relatively quickly. We have developed a protocol for creating simple microfluidic devices using a 3D printer and PDMS adhered to glass. We have also explored methods to overcome the size-limits of the 3D-printed master templates by using shrinkable polymers and modified channel geometries to create a flow-focusing channel. This relatively simple and lower-cost technique can now be scaled to more complicated device designs and applications.

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Evaluation of 3D Printing and Its Potential Impact on Biotechnology and the Chemical Sciences

Cited by 1,462 publications

References 153 publications

Hydrogel-laden paper scaffold system for origami-based tissue engineering

Hydrogel-laden paper scaffold system for origami-based tissue engineering

3D-Printed Autonomous Capillaric Circuits^†

Developing a Protocol for Creating Microfluidic Devices with a 3D Printer

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Evaluation of 3D Printing and Its Potential Impact on Biotechnology and the Chemical Sciences

Cited by 1,462 publications

References 153 publications

Hydrogel-laden paper scaffold system for origami-based tissue engineering

Hydrogel-laden paper scaffold system for origami-based tissue engineering

3D-Printed Autonomous Capillaric Circuits†

Developing a Protocol for Creating Microfluidic Devices with a 3D Printer

Contact Info

Product

Resources

About

3D-Printed Autonomous Capillaric Circuits^†