Evaluation of a commercial monoclonal antibody-based enzyme immunoassay for detection of adenovirus types 40 and 41 in stool specimens

Abstract

scribe an evaluation of the first commercial monoclonal antibody-based EIA for Ad-F viruses. MATERIALS AND METHODS Stool specimens. A total of 177 virologically characterized stool samples (Table 1) from children in North Manchester, United Kingdom, with gastroenteritis were tested. All samples were stored at-40°C before use. Electron microscopy and IEM. Stool samples were examined by negative stain electron microscopy after they were concentrated by ultracentrifugation. Viruses were identified

Detection, typing, and subtyping of enteric adenoviruses 40 and 41 from fecal samples and observation of changing incidences of infections with these types and subtypes

“…In the present report and in line with earlier studies (14,15,26,27), we described the development of a panel of MAbs that can be used for the rapid detection and typing of Ad4O and Ad4l. Some of these MAbs were also used in previous studies (32,36,40,41) (Table 1). In view of the close antigenic relationship between Ad4O and Ad4l in VN assays and the marked antigenic variability within these two types (9), there is a risk that anti-AdF MAbs would react with certain strains of both types or, alternatively, that they may not react with all the strains of the type concerned.…”

“…On the basis of these results and by using the same MAbs, an ELISA for the detection and typing of AdF was designed; this ELISA will soon become commercially available (40). The same MAbs were also applied successfully by others in typing enteric adenoviruses for diagnostic purposes (36) and in IEM analyses of fecal samples (41).…”

Laboratory identification of adenoviruses associated with gastroenteritis in Canada from 1983 to 1986

“…Given the importance of adenoviruses as etiologic agents of pediatric gastroenteritis, a number of assays have recently been developed for the direct detection of adenoviruses in stool specimens. Immunoassays based on the detection of the adenovirus group-specific antigen (2,12,16,21) do not distinguish between serotypes, whereas other immunoassays are designed specifically for the detection of serotypes 40 and 41 (7a, 14,23,28,29). To be able to evaluate these immunoassays comprehensively for their ability to identify adenoviruses associated with gastroenteritis, it is essential to know the full spectrum of adenovirus serotypes routinely present in stool specimens from symptomatic patients.…”

Astroviruses, Enteric Adenoviruses, and Other Gastroenteritis Viruses

“…Since EM cannot distinguish EAs from nonenteric serotypes, 94 immune electron microscopy (IEM) can enhance sensitivity and specificity of EA detection. [95][96][97][98] Enzyme immunoassays using monoclonal antibodies to types 40 and 41 and to the adenovirus hexon common to all serotypes have been developed. [98][99][100][101][102] These are the easiest, most rapid methods for detection 103 and have proven to be highly sensitive and specific compared to IEM.…”

“…[98][99][100][101][102] These are the easiest, most rapid methods for detection 103 and have proven to be highly sensitive and specific compared to IEM. 98 There are currently no commercial kits using DNA detection methods. Enteric adenoviruses grow in Graham 293 cells, a cell line transformed by adenovirus type 5.…”