Monoclonal antibody (MAb) preparations specific for the enteric adenoviruses of subgenus F (AdF) were generated and evaluated as typing reagents in virus neutralization tests and enzyme-linked immunosorbent assays (ELISAs). A panel of 11 genome types of adenovirus 40 (Ad40), 24 genome types of Ad4l, and 47 adenovirus prototype strains was used to determine the specificities of the MAbs in the two assays. In this way two MAbs, MAb 40-1 (anti-Ad40) and MAb 41-1 (anti-Ad4l) were selected. These two MAbs showed strict type

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scribe an evaluation of the first commercial monoclonal antibody-based EIA for Ad-F viruses. MATERIALS AND METHODS Stool specimens. A total of 177 virologically characterized stool samples (Table 1) from children in North Manchester, United Kingdom, with gastroenteritis were tested. All samples were stored at-40°C before use. Electron microscopy and IEM. Stool samples were examined by negative stain electron microscopy after they were concentrated by ultracentrifugation. Viruses were identified