Development and evaluation of monoclonal antibody-based immune electron microscopy for diagnosis of adenovirus types 40 and 41

Wood, David; Jong, J.C. de; Bijlsma, K; Avoort, H. G. A. M. Van Der

doi:10.1016/0166-0934(89)90051-7

Cited by 15 publications

(12 citation statements)

References 9 publications

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“…In previous reports, a predominance of AdV-41 over AdV-40 has been shown (10,11,14,19,39,41). This trend may be associated with the higher pathogenicity of AdV-41 in these communities (24,52). In contrast, studies in rural Bangladesh, the Netherlands, and Germany showed that the frequencies of these two serotypes were almost the same (8, 17).…”

Section: Discussionmentioning

confidence: 66%

“…AdV-41 has already been characterized by sequencing of the hexon gene, which classifies it into two genomic type clusters, GTC1 and GTC2. GTC1 includes strains D1, D25, D26, D27, and D28 and GTC2 contains strains D4, D12, and D22 (24,52). The existence of two GTCs reflects the accumulation of amino acid mutations in the HVRs of the hexon gene.…”

Section: Discussionmentioning

confidence: 99%

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Circulation of a Novel Pattern of Infections by Enteric Adenovirus Serotype 41 among Children below 5 Years of Age in Kolkata, India

et al. 2011

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Section: Discussionmentioning

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Circulation of a Novel Pattern of Infections by Enteric Adenovirus Serotype 41 among Children below 5 Years of Age in Kolkata, India

et al. 2011

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“…For serotyping of enteric Ad, EIA and immune EM have been developed, but they are not widely u. o available (Wood et al, 1989). Restriction endonurr clease of DNA has been found to be a very useful " m 10 technique for typing virus other than serotypes 1-7 and Ad variants that may not be identified by spe-z cies-specific antisera.…”

Section: Omentioning

confidence: 99%

“…Enteric Ads have fastidious culture characteristics and present a particular challenge in the diagnostic laboratory. This diagnostic difficulty has promoted considerable effort to enhance detection of enteric Ads, producing improved methods for the diagnosis of Ad40 and Ad41, including enzyme immunoassay (EIA) (August and Warford, 1987;Herrmann et al, 1987;Mortensson-Egnund and Kjeldsberg, 1986;Singh-Naz et al, 1988), dot-blot hybridization (Hammond et al, 1987;Kidd et al, 1985), and immune electron microscopy (EM) (Wood et al, 1989). Commercial monoclonal antibody-based enzyme immunoassay tests for enteric and all adenoviruses have become available for routine diagnosis of these viruses in diagnostic laboratories.…”

Section: Introductionmentioning

confidence: 99%

Comparison of detection methods for adenovirus from enteric clinical specimens

Ahluwalia

Scott-Taylor

Klisko

et al. 1994

Diagnostic Microbiology and Infectious Disease

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Fecal samples submitted for virus examination over July 1990 to June 1991 from children < 3 years of age were examined by electron microscopy (EM), virus culture (VC), and enzyme immunoassay [EIA, group-reactive and adenovirus (Ad) 40/41 specific; Cambridge BioScience] to compare the detection rate of adenovirus from pediatric fecal specimens. Ad isolates of serotypes 1-7 grown in HEp-2 or primary rhesus monkey kidney cells were identified by neutralization. Graham 293 cell cultures were used only when specimens were found to be positive for Ad by EM, type-specific Ad40/41 EIA, and for isolates not identified by neutralization. Ads grown in 293 cells were identified by DNA restriction endonuclease analysis. Of the 1187 specimens examined, 105 (9%) were found to be positive for Ad. VC detected 93, while 12 additional positives were detected by EM or EIA. The relative sensitivity of VC, EIA, and EM for the 105 specimens was 89% (93), 45% (47), and 35% (37), respectively. Among the 105 positive specimens, enteric Ad, nonenteric Ad, and untypeable Ad were 28% (29), 65% (68), and 7% (8), respectively. Of 37 EM positives, 62% (23) were enteric Ad; 27% (10) were nonenteric including serotypes 2, 3, 4, 5, 12, and 31, with 4, 1, 1, 2, 1, and 1 isolates of each type positive, respectively; and 11% (4) were detectable only by EM. Five isolates were identified as variant of Ad 2(3), Ad 3(1) and Ad 31(1). Over a 1-year period, a single Ad41 variant strain was the most frequently detected enteric Ad in Winnipeg, Manitoba, Canada.(ABSTRACT TRUNCATED AT 250 WORDS)

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“…Specific diagnosis was initially by electron microscopic examination of the stool for virus particles or extraction and analysis of DNA from the stool. Immunoassays using monoclonal antibodies specific for subtypes 40 and 41 have now facilitated diagnosis (Wood et al, 1989). Antigenic variation in surface epitopes may affect the sensitivity of the test, necessitating careful selection of a monoclonal antibody directed against a common antigen.…”

Section: Diagnosismentioning

confidence: 99%

Viruses

Schwab¹,

Shaw²

1993

Baillière's Clinical Gastroenterology

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Increased knowledge has been gained into the aetiology and pathogenesis of viral gastroenteritis during the past two decades. There are now thought to be four major subclassifications of gastroenteritis-causing viruses; these include rotavirus, enteric adenovirus, calicivirus, including Norwalk and Norwalk-like viruses, and astrovirus. The association of these agents with gastroenteritis has been made by their electron microscopic detection in stool and intestinal biopsy specimens from affected patients, the inability to detect the viruses after recovery from disease, and the subsequent development of immunoglobulin responses after infection; in some instances disease transmission was achieved in human volunteers. The association of these viral agents with gastroenteritis has facilitated the study of classification, epidemiology, immunity, diagnostic tests, methods of treatment and, most importantly, disease prevention strategies such as vaccine development for rotavirus. This chapter highlights the major features of these agents, with special attention being given to the pertinent molecular biology as well as current and future prospects for vaccination. Enteric viral infections of the gastrointestinal tract in patients with AIDS are also discussed.

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Development and evaluation of monoclonal antibody-based immune electron microscopy for diagnosis of adenovirus types 40 and 41

Cited by 15 publications

References 9 publications

Circulation of a Novel Pattern of Infections by Enteric Adenovirus Serotype 41 among Children below 5 Years of Age in Kolkata, India

Circulation of a Novel Pattern of Infections by Enteric Adenovirus Serotype 41 among Children below 5 Years of Age in Kolkata, India

Comparison of detection methods for adenovirus from enteric clinical specimens

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