Evaluation of a Disc Tube Methodology for Nano- and Ultrafiltration of Natural Dissolved Organic Matter (DOM)

Brockmeyer, Berit; Spitzy, Alejandro

doi:10.4236/ijoc.2013.31a002

Cited by 6 publications

(8 citation statements)

References 18 publications

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“…In the former, 90 L of Kalix River water was filtered through a 1-kDa Omega polyethersulfone UF membrane (Pall, Germany) according to the set-up of Brockmeyer and Spitzy [35]. The resulting 3.3 L of ultrafiltrate had a DOC concentration of 4408 (±24) μmolC L −1 (recovery rate 40%).…”

Section: Methodsmentioning

confidence: 99%

“…For the experiment, the stored, frozen water was thawed and separate aliquots of the 0.2-μm-filtered tDOM were concentrated by either tangential-flow ultrafiltration or lyophilization [34] . In the former, 90 L of Kalix River water was filtered through a 1-kDa Omega polyethersulfone UF membrane (Pall, Germany) according to the set-up of Brockmeyer and Spitzy [35] . The resulting 3.3 L of ultrafiltrate had a DOC concentration of 4408 (±24) μmolC L −1 (recovery rate 40%).…”

Section: Methodsmentioning

confidence: 99%

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Uncoupling of Bacterial and Terrigenous Dissolved Organic Matter Dynamics in Decomposition Experiments

et al. 2014

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The biodegradability of terrigenous dissolved organic matter (tDOM) exported to the sea has a major impact on the global carbon cycle, but our understanding of tDOM bioavailability is fragmentary. In this study, the effects of preparative tDOM isolation on microbial decomposition were investigated in incubation experiments consisting of mesocosms containing mesohaline water from the Baltic Sea. Dissolved organic carbon (DOC) consumption, molecular DOM composition, bacterial activities, and shifts in bacterial community structure were compared between mesocosms supplemented with riverine tDOM, either as filtered, particle-free river water or as a concentrate obtained by lyophilization/tangential ultrafiltration, and those containing only Baltic Sea water or river water. As shown using ultra-high-resolution mass spectrometry (15 Tesla Fourier-transform ion cyclotron resonance mass spectrometry, FT-ICR-MS) covering approximately 4600 different DOM compounds, the three DOM preparation protocols resulted in distinct patterns of molecular DOM composition. However, despite DOC losses of 4–16% and considerable bacterial production, there was no significant change in DOM composition during the 28-day experiment. Moreover, tDOM addition affected neither DOC degradation nor bacterial dynamics significantly, regardless of the tDOM preparation. This result suggested that the introduced tDOM was largely not bioavailable, at least on the temporal scale of our experiment, and that the observed bacterial activity and DOC decomposition mainly reflected the degradation of unknown, labile, colloidal and low-molecular weight DOM, both of which escape the analytical window of FT-ICR-MS. In contrast to the different tDOM preparations, the initial bacterial inoculum and batch culture conditions determined bacterial community succession and superseded the effects of tDOM addition. The uncoupling of tDOM and bacterial dynamics suggests that mesohaline bacterial communities cannot efficiently utilize tDOM and that in subarctic estuaries other factors are responsible for the removal of imported tDOM.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Uncoupling of Bacterial and Terrigenous Dissolved Organic Matter Dynamics in Decomposition Experiments

et al. 2014

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“…A 5-point calibration from 0.5 to 5 mg DOC/L was used. The error of measurement is less than 2% (for further analytical details see Brockmeyer and Spitzy, 2013).…”

Section: Docmentioning

confidence: 99%

Biogeochemical Regeneration of a Nodule Mining Disturbance Site: Trace Metals, DOC and Amino Acids in Deep-Sea Sediments and Pore Waters

et al. 2018

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Increasing interest in deep-sea mineral resources, such as polymetallic nodules, calls for environmental research about possible impacts of mineral exploitation on the deep-sea ecosystem. So far, little geochemical comparisons of deep-sea sediments before and after mining induced disturbances have been made, and thus long-term environmental effects of deep-sea mining are unknown. Here we present geochemical data from sediment cores from an experimental disturbance area at 4,100 m water depth in the Peru Basin. The site was revisited in 2015, 26 years after a disturbance experiment mimicking nodule mining was carried out and compared to sites outside the experimental zone which served as a pre-disturbance reference. We investigated if signs of the disturbance are still visible in the solid phase and the pore water after 26 years or if pre-disturbance conditions have been re-established. Additionally, a new disturbance was created during the cruise and sampled 5 weeks later to compare short-and longer-term impacts. The particulate fraction and pore water were analyzed for major and trace elements to study element distribution and processes in the surface sediment. Pore water and bottom water samples were also analyzed for oxygen, nitrate, dissolved organic carbon, and dissolved amino acids, to examine organic matter degradation processes. The study area of about 11 km 2 was found to be naturally more heterogeneous than expected, requiring an analysis of spatial variability before the disturbed and undisturbed sites can be compared. The disturbed sites exhibit various disturbance features: some surface sediments were mixed through, others had the top layer removed and some had additional material deposited on top. Pore water constituents have largely regained pre-disturbance gradients after 26 years. The solid phase, however, shows clear differences between disturbed and undisturbed sites in the top 20 cm so that the impact is still visible in the plowed tracks after 26 years. Especially the upper layer, usually rich in manganese-oxide Paul et al.Biogeochemical Regeneration After Mining Disturbance and associated metals, such as Mo, Ni, Co, and Cu, shows substantial differences in metal distribution. Hence, it can be expected that disturbances from polymetallic nodule mining will have manifold and long-lasting impacts on the geochemistry of the underlying sediment.

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“…Pore-water samples were taken by rhizons and stored frozen before analyses (see methods in Paul et al, 2018). 18 water samples taken off Namibia were separated into two size classes by ultrafiltration (Brockmeyer and Spitzy, 2013). The size classes 50 kDa-0.7 µm and 1 kDa-0.7 µm were used for AA analyses.…”

Section: Samplingmentioning

confidence: 99%

What can we learn from amino acids about oceanic organic matter cycling and degradation?

Gaye

Lahajnar

Harms

et al. 2021

Preprint

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Abstract. Amino acids (AA) mainly bound in proteins are major constituents of living biomass and non-living organic material in the oceanic particulate and dissolved organic matter pool. Uptake and cycling by heterotrophic organisms lead to characteristic changes in AA composition so that AA based biogeochemical indicators are often used to elucidate processes of organic matter cycling and degradation. We analyzed particulate AA in a large sample set collected in various oceanic regions covering sinking and suspended particles in the water column, sediment samples as well as dissolved AA from water column and pore water samples. The aim of this study was to test and improve the use of AA derived biogeochemical indicators as proxies for organic matter sources and degradation, and to better understand particle dynamics and interaction between the dissolved and particulate organic matter pools. A principal component analysis (PCA) of all data delineates diverging AA compositions of sinking and suspended particles with increasing water depth. A new sinking particle and sediment degradation indicator (SDI) allows a fine-tuned classification of sinking particles and sediments with respect to the intensity of degradation, which is associated with changes of bulk δ15N ratios. This new indicator furthermore is sensitive to sedimentary redox conditions and can be used to detect past anoxic early diagenesis. A second indicator emerges from the AA spectra of suspended particulate matter (SPM) in the epipelagic and that of the meso- and bathypelagic ocean and is a residence time indicator (RTI). The characteristic changes in AA patterns from shallow to deep SPM are recapitulated in the AA spectra of the dissolved organic matter (DOM) pool, so that deep SPM is more similar to DOM than to any of the other organic matter pools. This implies that there is equilibration between finely dispersed SPM and DOM in the deep sea, which may be driven by microbial activity combined with annealing and fragmentation of gels. As these processes strongly depend on physico-chemical conditions in the deep ocean, changes in quality and degradability of DOM may strongly affect the relatively large pool of suspended and dissolved AA in the ocean that amounts to 15 Pg amino acid carbon (AAC) and 89 ± 29 Pg AAC, respectively.

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Evaluation of a Disc Tube Methodology for Nano- and Ultrafiltration of Natural Dissolved Organic Matter (DOM)

Abstract:
The performance of a disc tube (DT) methodology, originally designed for reverse osmosis (RO) in desalination, and applied here for ultrafiltration (UF) and nanofiltration (NF) of dissolved organic matter (DOM) in a set of fresh (0 ppt), brackish (10 ppt) and saline (30 Show more

Cited by 6 publications

References 18 publications

Uncoupling of Bacterial and Terrigenous Dissolved Organic Matter Dynamics in Decomposition Experiments

Uncoupling of Bacterial and Terrigenous Dissolved Organic Matter Dynamics in Decomposition Experiments

Biogeochemical Regeneration of a Nodule Mining Disturbance Site: Trace Metals, DOC and Amino Acids in Deep-Sea Sediments and Pore Waters

What can we learn from amino acids about oceanic organic matter cycling and degradation?

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Evaluation of a Disc Tube Methodology for Nano- and Ultrafiltration of Natural Dissolved Organic Matter (DOM)

Abstract: The performance of a disc tube (DT) methodology, originally designed for reverse osmosis (RO) in desalination, and applied here for ultrafiltration (UF) and nanofiltration (NF) of dissolved organic matter (DOM) in a set of fresh (0 ppt), brackish (10 ppt) and saline (30 Show more

Cited by 6 publications

References 18 publications

Uncoupling of Bacterial and Terrigenous Dissolved Organic Matter Dynamics in Decomposition Experiments

Uncoupling of Bacterial and Terrigenous Dissolved Organic Matter Dynamics in Decomposition Experiments

Biogeochemical Regeneration of a Nodule Mining Disturbance Site: Trace Metals, DOC and Amino Acids in Deep-Sea Sediments and Pore Waters

What can we learn from amino acids about oceanic organic matter cycling and degradation?

Contact Info

Product

Resources

About

Abstract:
The performance of a disc tube (DT) methodology, originally designed for reverse osmosis (RO) in desalination, and applied here for ultrafiltration (UF) and nanofiltration (NF) of dissolved organic matter (DOM) in a set of fresh (0 ppt), brackish (10 ppt) and saline (30 Show more