Evaluation of a focused sonication probe for arsenic speciation in environmental and biological samples

Sanz, E.; Muñoz-Olivas, Riánsares; Cámara, Carmen

doi:10.1016/j.chroma.2005.08.012

Cited by 70 publications

(54 citation statements)

References 37 publications

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“…Comparisons between the certified values and the measured concentrations of AsB and DMA in the extract suggest that the extraction efficiencies were 67% for AsB and 80% for DMA. This degree of extraction efficiency is commonly experienced by others, reporting extraction efficacy ranging from 30% to 80% [34,35,54].…”

Section: Extraction Methodsmentioning

confidence: 67%

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Enzyme-assisted extraction and liquid chromatography mass spectrometry for the determination of arsenic species in chicken meat

Liu

Peng

et al. 2015

Analytica Chimica Acta

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Section: Extraction Methodsmentioning

confidence: 67%

“…Procedures for ultrasonication were modified from the method of Sanz et al [54]. A Misonix sonicator 4000 (Qsonica, CT, USA) was used.…”

Section: Extraction Of Arsenic Speciesmentioning

confidence: 99%

Enzyme-assisted extraction and liquid chromatography mass spectrometry for the determination of arsenic species in chicken meat

Liu

Peng

et al. 2015

Analytica Chimica Acta

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“…19,20 For example, Huerga et al 19 observed that the optimum extraction time of arsenic from river sediment was 60 s by using phosphate buffer to extract the analyte. In the present work, previous addition of concentrated HCl and sonication in 20% (v/v) HCl may have shortened the period of sonication.…”

Section: Methods Developmentmentioning

confidence: 99%

Arsenic Determination in Marine Sediment Using Ultrasound for Sample Preparation

et al. 2007

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This work deals with As determination in marine sediment using ultrasound for sample preparation. It is shown that As can be quantitatively extracted from marine sediment using 20% (v/v) HCl and sonication. The slurry is centrifuged and the analyte is determined in the supernatant by hydride generation atomic absorption spectrometry (HG AAS). A flow injection (FI) system is employed for hydride generation, with 0.5% (m/v) NaBH4 used as reducdant and a 20% (v/v) HCl used as sample carrier. The limit of quantification is 1.6 μg g -1 of As, which is based on 800 μl of sample solution and 0.200 g of sample mass in a volume of 50 mL. Certified and non certified marine sediment samples were analyzed; the results were in accordance with the certified or reference values. Speciation analysis by HPLC-ICP-MS showed that As(V) is the only detectable As species present in the supernatant of the centrifuged sample.

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“…A mobile phase with a high pK a value and a high pH range would be desirable for increased the ionization and consequently better separation. Several authors 7,14,15 have been able to separate these five arsenic species using an anion exchange column with a neutral phosphate buffer HPO 4 -2 and H 2 PO 4 -1 as a mobile phase (pK a 6.8). When we tried to use this buffer at pH 7.5 as a mobile phase with a PRP X-100 column, As III and arsenobetaine were not separated.…”

Section: Chromatographic Separationmentioning

confidence: 99%

“…The inorganic arsenic species arsenite and arsenate have been related to an increased risk of cancer as well as to cardiovascular diseases, whereas methylated forms of arsenic such as methylarsonic acid (MMA) and dimethylarsinic acid (DMA) are significantly less toxic. Other species such as arsenobetaine and arsenocholine are essentially non-toxic 7 . The major route for the excretion of As is via urine and for human biomonitoring urine is mostly recommended for the speciation of As.…”

Section: Introductionmentioning

confidence: 99%

Methodological Validation for the Determination of Toxic Arsenic Species in Human Urine Using HPLC With Icp-MS

Muñoz

Meneses

Pismante

et al. 2014

J. Chil. Chem. Soc.

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There is a significant amount of arsenic species in the environment and in biological systems; however, the toxicity and possibly the carcinogenicity of ingested arsenic depend on the species and the oxidation state. Therefore, analytical methodologies and techniques that are fast and robust, that involve highly efficient separation and are powerful tools of detection are necessary for the correct quantification of individual arsenic species. The field of speciation analysis of arsenic in this area has grown rapidly in recent years, especially with the application of liquid chromatography and mass spectrometry with inductively coupled plasma. This study aimed to develop and apply this technique to human urine samples in order to reduce the treatment needed for speciation, maintain maximum recovery and avoid interconversion and / or degradation between species. In order to optimize the separation of species, several experiments were performed by preparing the mobile phase at different pH values. The optimal pH value and the linearity of the method were determined and the methodology was validated using a Certified Reference Material. Student`s t-test and Fisher F-test for determination of accuracy and precision were applied. A simple, rapid and reliable method for the separation and quantification of several species of arsenic, As III (Arsenite), As V (Arsenate), MMA (Monomethyl Arsonic Acid), DMA (Dimethyl Arsinic Acid), AB (Arsenobetaine) was developed in human urine samples with minimal prior sample preparation. The time required for sample processing and isocratic chromatographic analysis is about 15 minutes. Detection limits of 0.020 µgL -1 of AsB, 0.020 µgL -1 of As III, 0.040 µgL -1 of DMA, 0.060 µgL -1 of MMA and 0.060 µgL -1 of As V were achieved under the analytical conditions mentioned above.

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Evaluation of a focused sonication probe for arsenic speciation in environmental and biological samples

Cited by 70 publications

References 37 publications

Enzyme-assisted extraction and liquid chromatography mass spectrometry for the determination of arsenic species in chicken meat

Enzyme-assisted extraction and liquid chromatography mass spectrometry for the determination of arsenic species in chicken meat

Arsenic Determination in Marine Sediment Using Ultrasound for Sample Preparation

Methodological Validation for the Determination of Toxic Arsenic Species in Human Urine Using HPLC With Icp-MS

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