Evaluation of a low-cost, automated pyrolysis-mass spectrometer

Aries, R. E.; Gutteridge, C. S.; Ottley, T. W.

doi:10.1016/0165-2370(86)85001-x

Cited by 69 publications

(15 citation statements)

References 9 publications

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“…The recent application of pyrolysis-mass spectrometry for strain discrimination of CNS has given encouraging results (91,92). In this technique, the organisms are pyrolyzed, the pyrolysates are examined by mass spectrometry, and the results are analyzed and compared mathematically to produce a dendrogram (9). Investigators have found pyrolysis-mass spectrometry to be comparable to typing schemes that incorporate antibiogram, biotype, and plasmid analysis (91,92).…”

Section: Conventional Methodsmentioning

confidence: 99%

Update on clinical significance of coagulase-negative staphylococci

1994

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The clinical significance of coagulase-negative Staphylococcus species (CNS) continues to increase as strategies in medical practice lead to more invasive procedures. Hospitalized patients that are immunocompromised and/or suffering from chronic diseases are the most vulnerable to infection. Since CNS are widespread on the human body and are capable of producing very large populations, distinguishing the etiologic agent(s) from contaminating flora is a serious challenge. For this reason, culture identification should proceed to the species and strain levels. A much stronger case can be made for the identification of a CNS etiologic agent if the same strain is repeatedly isolated from a series of specimens as opposed to the isolation of different strains of one or more species. Strain identity initially can be based on colony morphology, and then one or more molecular approaches can be used to gain information on the genotype. Many of the CNS species are commonly resistant to antibiotics that are being indicated for staphylococcal infections, with the exception of vancomycin. The widespread use of antibiotics in hospitals has provided a reservoir of antibiotic-resistant genes. The main focus on mechanisms of pathogenesis has been with foreign body infections and the role of specific adhesins and slime produced by Staphylococcus epidermidis. Slime can reduce the immune response and opsonophagocytosis, thereby interfering with host defense mechanisms. As we become more aware of the various strategies used by CNS, we will be in a better position to compromise their defense mechanisms and improve treatment.

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Section: Conventional Methodsmentioning

confidence: 99%

Update on clinical significance of coagulase-negative staphylococci

1994

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“…Prutech (London) developed a low cost quadrupole mass spectrometer specifically designed for automated Py-MS analysis (Aries et al, 1986), marketed by Horizon Instruments (Heathfield, Sussex). We were asked to assess a prototype instrument in late 1986, and have investigated various microbiological applications, including Py-MS characterisation of bacterial genera that are difficult to characterise by conventional means.…”

Section: Introductionmentioning

confidence: 99%

A pyrolysis mass spectrometry study of fusobacteria

Magee¹,

Hindmarch²,

Bennett³

et al. 1989

Journal of Medical Microbiology

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“…Inter-strain comparison by PyMS is based on mathematically derived quantitative differences between spectrograms, expressing similarities and differences in relative terms [9]. It is, therefore, essential to include epidemiologically unrelated control strains so that the significance of these relative differences can be estimated.…”

Section: Discussionmentioning

confidence: 99%

“…Each distinguishable pattern obtained was assigned an arbitrary number (PI, P2, P3, and so on) to allow comparisons between isolates from the same patient and between patients. The apparatus, technique and principles involved have been described previously [9]. Briefly, a well-separated colony of the isolate to be tested was identified and sampled with a flamed straight wire.…”

Section: Plasmid Profilesmentioning

confidence: 99%

Rapid inter-strain comparison by pyrolysis mass spectrometry of coagulase-negative staphylococci from persistent CAPD peritonitis

et al. 1991

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SUMMARYPyrolysis mass spectrometry (PyMS) was used as a method of rapid inter-strain comparison of 19 isolates of coagulase-negative staphylococci from episodes of CAPD peritonitis. Thirteen isolates were from multiple, but distinct, episodes of peritonitis in 6 patients and the remaining 6 isolates were from 6 patients with single episodes. The results, expressed in terms of identity/non-identity of strains, were compared with those obtained using an established typing system comprising an extended antibiogram, determination of biotype and plasmid profile analysis. The PyMS results for inter-strain comparison were in agreement with the reference typing scheme results. PyMS can be used in this setting to rapidly obtain evidence that persistent infection is/is not likely to be due to the same organism, although it cannot be used for formal typing. The results by both methods showed that serial, apparently distinct, episodes of peritonitis over periods as long as 120 days may be due to the same strain of coagulase-negative staphylococcus. Clinically based distinctions between recurrence of infection (same strain) and re-infection (different strains) may not always be supported by the microbiological evidence.

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Evaluation of a low-cost, automated pyrolysis-mass spectrometer

Cited by 69 publications

References 9 publications

Update on clinical significance of coagulase-negative staphylococci

Update on clinical significance of coagulase-negative staphylococci

A pyrolysis mass spectrometry study of fusobacteria

Rapid inter-strain comparison by pyrolysis mass spectrometry of coagulase-negative staphylococci from persistent CAPD peritonitis

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