Evaluation of a Mobile Phone-Based Microscope for Screening of Schistosoma haematobium Infection in Rural Ghana

Bogoch, Isaac I.; Koydemir, Hatice Ceylan; Tseng, Derek; Ephraim, Richard K. D.; Duah, Evans; Tee, Joseph; Andrews, Jason R; Ozcan, Aydogan

doi:10.4269/ajtmh.16-0912

Cited by 51 publications

(38 citation statements)

References 19 publications

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“…In this design, polarization light microscopy on the cellphone camera unit allowed detection of malaria‐infected regions of tissues based on imaging of hemozoin, a malaria pigment. Another platform was developed for Schistosoma haematobium infections in rural Ghanaian schools . A library of 60 patient samples from randomly selected students was screened by mobile phone diagnostics with 72.1% sensitivity.…”

Section: Applications In Field Research and Diagnosticsmentioning

confidence: 99%

Portable Multiplex Optical Assays

Coşkun

Topkaya

Yetisen

et al. 2018

Advanced Optical Materials

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performed at home and results can be integrated by online interfaces. [5] The test results and diagnostic information can be analyzed by the medical professionals from remote locations, improving the connectivity of central hubs to the public venues.Numerous scientific discoveries have been made in advanced settings and wellequipped laboratories. The necessity and complexity of these environments limit access to research equipment worldwide. Thus, decentralization of research laboratories has become an alternative solution to improve access to experimentation in field conditions. For instance, a paper origami microscope [6] that fit into a palm of a student made a powerful investigation tool for environment and materials. A detailed analysis of sound recordings by smartphones enabled identification of distinct mosquitoes. [7] These fielddeployable devices and platforms have stimulated emergence of citizen scientists, a population that collectively performs scientific inquiries in public settings.Rich biomolecular measurements from field-portable devices improve the quality of results in terms of accuracy and depth. Monitoring multiple blood markers has enabled diagnostic precision and thorough scientific investigation of blood constituent in patients. Therefore, field-deployable devices should ideally have multiplexed detection of target specimens. Design of such multiplexed detection technologies requires decent complexity at low cost for wide-scale adoptability.In this article, we provide an overview of field-compatible devices with high parameter analysis capabilities for both health monitoring purposes and scientifically enlightening Global health issues are increasingly becoming critical with high fatality rate due to chronic and infectious diseases. Emerging technologies aim to address these problems by understanding the causes of lethal conditions and diagnosing symptoms at early stage. Existing commercial diagnostics primarily focus on single-plex assays due to ease-of-use, simplicity in analysis, and amenability to mass manufacturing. Many research grade devices have utilized only a few molecular and morphological signatures in bodily fluids. However, multiplex devices can improve accuracy, sensitivity, and scalability of research and diagnostic devices. This review presents multiplex assays that utilize optical, electrical, and chemical methods and materials that have the potential to improve portable point-of-care diagnostics. The critical role of emerging optical and complementary assays with multiple contrast mechanisms is investigated to enable highly multiplex analysis in field settings. Multiparameter portable devices for field applications toward health monitoring, food testing, air quality monitoring, and microanalysis in other extreme conditions are examined. Current trends indicate the need for validation of health diagnosis based on a large number of biomarkers in randomized clinical trials. Advanced digital analysis, crowdsourced solutions, and robust user interfaces will become an integral ...

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Section: Applications In Field Research and Diagnosticsmentioning

confidence: 99%

Portable Multiplex Optical Assays

Coşkun

Topkaya

Yetisen

et al. 2018

Advanced Optical Materials

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“…This cost-effective and field-portable smartphone-based microscope has a spatial resolution of approximately 0.87 μm and uses a translation stage to scan a microscope slide for the presence of Schistosoma haematobium eggs. The field experiments conducted with this mobile phone microscope revealed a sensitivity of 72.1%, a specificity of 100%, a positive-predictive value of 100% and a negative-predictive value of 57.1% [11].…”

Section: Editorial Koydemir and Ozcan Future Science Groupmentioning

confidence: 99%

“…Field testing of this prototype was conducted in Cameroon, Central Africa using 33 Loa-infected patients, demonstrating 94% specificity and 100% sensitivity in comparison to manual thick smear counts. Another recent study [11] evaluated the performance of a smartphone-based bright-field microscope for labelfree detection of Schistosoma spp. eggs in urine samples in rural areas of Accra, Ghana, Africa.…”

Section: Editorial Koydemir and Ozcan Future Science Groupmentioning

confidence: 99%

“…In fact, mobile phone-based imaging and sensing platforms have already been used in a variety of applications, including clinical chemistry, biomedical and environmental monitoring [3][4][5], food analysis [6][7][8], detection of different types of chemical and biological analytes such as cells, parasites [9], bacteria [10], eggs [11], proteins [12], various biomarkers [6,7], nanoparticles [13] and even nucleic acids [14][15][16]. In many of these platforms, processing of the acquired data and the resulting computational analysis are done either on the smartphone or over a local or remote server using a customdeveloped smartphone application.…”

Section: Mobile Phone-enabled Measurement Tools For Research and Clinicmentioning

confidence: 99%

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Mobile Phones Create new Opportunities for Microbiology Research and Clinical Applications

Koydemir

Ozcan

2017

Future Microbiol.

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KEYWORDSTogether with their cost-effectiveness, wide-spread use, computational power and connectivity, mobile phones have the potential to transform traditional uses of imaging, sensing and diagnostic systems, especially for point-of-care applications and field settings.In fact, mobile phone-based imaging and sensing platforms have already been used in a variety of applications, including clinical chemistry, biomedical and environmental monitoring [3][4][5], food analysis [6][7][8] [12], various biomarkers [6,7], nanoparticles [13] and even nucleic acids [14][15][16]. In many of these platforms, processing of the acquired data and the resulting computational analysis are done either on the smartphone or over a local or remote server using a customdeveloped smartphone application. In some cases, subcomponents of the mobile phone peripherals, for example, the complementary metal-oxide-semiconductor (CMOS) imager chip of the camera module, can also be used for advanced microscopy and cytometry applications [17][18][19], in which case a laptop computer or a tablet can be used for image reconstruction and related analysis.

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“…Here is the essence of portable and mobile phonebased microscope. Some mobile-phone-based microscope [1] are being already implemented in the rural area of Africa for the diagnosis of blood flukes (Schistosoma) [2], malaria [3] like diseases. Also, there have been feasibility study [4] of the mobile phonebased microscope for using in histopathological purposes.…”

Section: Introductionmentioning

confidence: 99%

Feasibility Study on Blood Cell Counting Using Mobile Phone-Based Portable Microscope

Waliullah

2018

IJCBR

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Objectives: To check the feasibility of using the mobile phone-based microscope for blood cell counting from human blood histological sample. Methodology: A feasibility study was performed by imaging blood histology samples with one novel type of microscope “Foldscope” and image compared with a conventional microscope in the laboratory facility. The image acquired from both modalities were processed further and compared and analyzed. Results: Mobile phone-based microscope acquired images were observed and compared with a conventional microscope and found the blood cell counting feasibility and morphology analysis of the blood histology sample were significantly similar as of conventional light microscope images. Conclusion: By comparing the image from both microscopes, it could be stated that this method is feasible for human blood histopathological sample investigations like blood cell counting and morphology analysis especially in the low resource area or in case of any emergency situations.

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Evaluation of a Mobile Phone-Based Microscope for Screening of Schistosoma haematobium Infection in Rural Ghana

Cited by 51 publications

References 19 publications

Portable Multiplex Optical Assays

Portable Multiplex Optical Assays

Mobile Phones Create new Opportunities for Microbiology Research and Clinical Applications

Feasibility Study on Blood Cell Counting Using Mobile Phone-Based Portable Microscope

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