Evaluation of a multiplex assay for adipokine concentrations in obese children

Martos‐Moreno, Gabriel Ángel; Burgos‐Ramos, Emma; Canelles, Sandra; Argente, Jesús; Barrios, Vicente

doi:10.1515/cclm.2010.276

Cited by 19 publications

(27 citation statements)

References 26 publications

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“…Moreover, use of the multiplexed assay has been criticized in the scientific literature 43. The multiplexed bead immunoassay allows the simultaneous detection of adipokines in small blood samples which may be particularly useful when samples are difficult to obtain.…”

Section: Discussionmentioning

confidence: 99%

“…The multiplexed bead immunoassay allows the simultaneous detection of adipokines in small blood samples which may be particularly useful when samples are difficult to obtain. The use of this approach has been validated and found to be useful for leptin, adiponectin, and insulin in the evaluation of changes in obesity markers following weight reduction 43. The relationship between the multiplexed bead immunoassay when compared with the radioimmunoassay or enzyme-linked immunoassay for other adipokines such as resistin, IL-6, and TNF-α, were reported to be quite weak.…”

Section: Discussionmentioning

confidence: 99%

“…These differences could be explained by differences in antibody pairs and sample diluents, as well as the low effects of low concentrations of these adipokines. In addition, assay sensitivity remains an issue when compared with ultrasensitive enzyme-linked immunoassay methods 43. However, the ability of multiplexed assays to detect adipokines of a broader dynamic range than enzyme-linked immunoassays, as well as its greater cost-effectiveness, time efficiency, requirement of smaller sample volumes, and the removal of interassay variability suggest that this approach is still to be considered a powerful tool, albeit with the above limitations.…”

Section: Discussionmentioning

confidence: 99%

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Effect of weight loss on adipokine levels in obese patients

Rolland¹,

Hession²,

Broom³

2011

DMSO

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Background:Adipose tissue functions as an endocrine organ by releasing adipokines which have important roles in the regulation of inflammation and insulin sensitivity. Although there is evidence of improvement in circulating levels of adipokines with weight loss, few studies relate such changes to specific diets. We investigated the effects of weight loss achieved by two different diets on circulating adipokine levels in obese individuals.Methods:A total of 120 obese patients (body mass index ≥ 35 kg/m2) underwent a three-month screening period on a low-fat, reduced-calorie diet. Patients failing to achieve a 5% weight loss using this approach were randomly allocated to either a low carbohydrate/high protein diet (n = 17) or to a commercial very low calorie diet (LighterLife®, n = 14) for a period of nine months.Results:At nine months, a significant weight loss was only maintained for Lighter-Life® (−32.3 ± 22.7 kg, P < 0.0001) but not on the low carbohydrate/high protein diet. Changes in adiponectin (15.8 ± 17.1 ng/mL versus −0.8 ± 6.2 ng/mL, P = 0.003) and leptin (−17.6 ± 24.3 ng/mL versus −3.0 ± 9.2 ng/mL, P = 0.049) at nine months were significantly greater for LighterLife® than for the low carbohydrate/high protein diet, which may reflect greater weight loss and decrease in fat mass. Changes in tumor necrosis factor-alpha, interleukin-6, and plasminogen activator inhibitor type 1 did not differ significantly between the dietary interventions at nine months.Conclusion:A significant weight loss of 23.8% from baseline weight was observed using a very low calorie diet and resulted in significant improvements in circulating levels of leptin, plasminogen activator inhibitor type 1, and adiponectin, which are likely to be due to weight loss and not macronutrient intake.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Effect of weight loss on adipokine levels in obese patients

Rolland¹,

Hession²,

Broom³

2011

DMSO

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show abstract

“…The acceptance of this technique depends on comparable results to those achieved by using classical techniques, accepted as the "gold standard" in the laboratory (165,166). A limitation in some MBIAs is the interpretation and reporting of analyte values at extremely low concentrations.…”

Section: Multiplex Bead Arrays -Strengths and Weaknessesmentioning

confidence: 99%

Aspects of inflammation, angiogenesis and coagulation in preeclampsia

Boij¹

2016

Linköping University Medical Dissertations

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“…In addition to these studies, development of a new MBIA or the inclusion of a new analyte to a pre-existent multiplexed panel also requires the analysis of classical assay characteristics, as sensitivity, specificity, precision, recovery and linearity, among others (Krouwer et al, 2002;Liu et al, 2005;Dossus et al, 2008;Martos-Moreno et al, 2010).…”

Section: Assay Guidelines and Technical Considerationsmentioning

confidence: 99%