Evaluation of a new laboratory model for pulp healing: preliminary study

Simon, Stéphane; Cooper, Paul R.; Smith, Anthony J.; Picard, B; Ifi, C. Naulin; Berdal, Ariane

doi:10.1111/j.1365-2591.2008.01433.x

Cited by 58 publications

(67 citation statements)

References 34 publications

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“…Due to microleakage, after 6 months, most of the Ca(OH) 2 capping material disintegrates and disappears (Cox et al 1996). Similar molecular events have been identified when other Ca(OH) 2 capping agents were used (Simon et al 2008). After mild necrosis, the cells proliferate.…”

Section: Introductionsupporting

confidence: 70%

Reactionary and reparative dentin formation after pulp capping: Hydrogel vs. Dycal

et al. 2016

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Background: After indirect capping, injured odontoblasts generate reactionary dentin, whereas after direct capping of a pulp exposure pulp, cells stimulate the formation of reparative dentin. The aim of this study was to evaluate and compare the effects of two direct capping agents on pulp tissue reactions: Hydrogel (a bovine serum albumin (BSA)/glutaraldehyde,) and Dycal (a calcium hydroxide-based capping agent). Methods: In 6-week-old male Sprague-Dawley rats, occlusal cavities were drilled in the first maxillary molars, and the pulps were exposed. In one of the groups, 24 right molars were capped with Hydrogel (G1), whereas in the other group 24 M were capped with Dycal (G2). After 1 to 4 weeks, the rats were anaesthetized intraperitoneally (six rats per group) and perfused intracardiacally with 4 % paraformaldehyde fixative. Maxillary molar's blocks were demineralized with a 4.13 % EDTA solution, embedded in paraffin, and the sections were histologically stained. Measurements of the thickness of reactionary dentin and area of inflammation were measured with ImageJ software. Results were compared with Kruskal Wallis and Mann Whitney U tests at p = 0.05. Results: One week after Dycal capping, a statistically significant large number of aggregates of pulp cells enlightened pulpal inflammation compared to Hydrogel. At 2-3 weeks, reactionary dentin formation was increased at the periphery of the pulp chamber. After 4 weeks, a dentinal bridge sealed partially the pulp exposure, while tunnel defects persisting across reparative osteodentin. In contrast, 1 week after Hydrogel capping, inflammation was barely detectable. Hydrogel induced the massive apposition of reactionary dentin at the pulp periphery, and reparative dentin was developing within the pulp. The degradation of Hydrogel releases glutaraldehyde acting on pulp cells as a fixative and consequently favoring BSA bioactivity. Conclusion: After Hydrogel capping, nemosis stimulates pulp mineralization, improving reactionary and reparative dentin formation. In contrast, the highly alkaline compound Dycal produced inflammation within the pulp. The differences between the two capping agents suggest that Hydrogel might present some clinical advantages over Dycal.

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Section: Introductionsupporting

confidence: 70%

Reactionary and reparative dentin formation after pulp capping: Hydrogel vs. Dycal

et al. 2016

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“…66,67 The odontoblast-like nature of the differentiated cells has been shown in a mouse model by staining for dentin sialophosphoprotein (DSPP) in the newly formed dentin bridge. 68 These responses are consistent with the similar clinical results obtained when MTA is compared with calcium hydroxide as a pulp-capping agent, although MTA typically shows less pulpal infl ammation and improved dentin bridging with fewer discontinuities. Perhaps the more rapid and enhanced response to MTA is the result of its very slow setting reaction, which provides comparatively more time for bioactive molecules to be solubilized from the dentin and made available for stimulating appropriate cells.…”

Section: Potential Stimulation Of Repair/healing By Current Dental Masupporting

confidence: 87%

Can interaction of materials with the dentin-pulp complex contribute to dentin regeneration?

2010

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Understanding outcomes of the interaction between a dental material and tooth tissue is important in terms not only of biocompatibility but also of the potential for the material to modulate the response of the tissue. This interaction is influenced by many factors, including the chemistry of the material and any of its eluted components or degradation products, and the manner in which the tissue responds to these agents. Past studies of this interaction have primarily been aimed at identifying cytotoxic effects. More recently, investigations have focused on specific cellular responses, and in particular, on understanding how the materials themselves actually may contribute to regenerative processes in the tooth. Recent work has demonstrated the solubilization of proteins from dentin exposed to certain materials, such as calcium hydroxide, mineral trioxide aggregate, and acidic solutions that relate to those used in dentin bonding agents, with the subsequent modulation by these proteins of gene expression in odontoblast-like cells. This work suggests that dentin bridge formation under such materials may be stimulated through this process. Thus, there is much merit in examining both how new dental materials can be developed and how more traditional ones can be modified to preferentially stimulate regenerative processes when preferred. This review summarizes current knowledge about the potential beneficial effects derived from the interaction of dental materials with the dentin-pulp complex, as well as potential future developments in this exciting field.

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“…Inducing effect for angiogenic factor secretion (23) and granulation tissue formation (29), which was related with wound healing, were also noted. MTA supports healing of pulp by being a biocompatible barrier that protects pulp from exposure to different physical and chemical stress conditions and provides calcium ions that are necessary for mineralization (30)(31)(32). Enamel extracellular matrix proteins in the form of EMD have been used for the regeneration of periodontal tissues because it triggers osteogenesis and mineralization of the tissues at the site of application (33).…”

Section: Discussionmentioning

confidence: 99%

Effect of Dental Materials Calcium Hydroxide–containing Cement, Mineral Trioxide Aggregate, and Enamel Matrix Derivative on Proliferation and Differentiation of Human Tooth Germ Stem Cells

Güven¹,

Yalvaç²,

Şahın³

et al. 2011

Journal of Endodontics

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Evaluation of a new laboratory model for pulp healing: preliminary study

Abstract: This is the first description of the utilization of a murine model for study of in vivo pulpal repair. This approach provides a novel opportunity to enable the use of genetically modified animals to explore cellular and molecular processes during reparative events.

Cited by 58 publications

References 34 publications

Reactionary and reparative dentin formation after pulp capping: Hydrogel vs. Dycal

Reactionary and reparative dentin formation after pulp capping: Hydrogel vs. Dycal

Can interaction of materials with the dentin-pulp complex contribute to dentin regeneration?

Effect of Dental Materials Calcium Hydroxide–containing Cement, Mineral Trioxide Aggregate, and Enamel Matrix Derivative on Proliferation and Differentiation of Human Tooth Germ Stem Cells

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