Evaluation of a new modified QuEChERS method for the monitoring of carbamate residues in high‐fat cheeses by using UHPLC–MS/MS

Abstract

A simple and efficient method for the determination of 28 carbamates in high-fat cheeses is proposed. The methodology is based on a modified quick, easy, cheap, effective, rugged, and safe procedure as sample treatment using a new sorbent (Z-Sep ) followed by ultra-high performance liquid chromatography with tandem mass spectrometry determination. The method has been validated in different kinds of cheese (Gorgonzola, Roquefort, and Camembert), achieving recoveries of 70-115%, relative standard deviations lower than 13% and limits of quantification lower than 5.4 μg/kg, below the maximum residue levels tolerated for these compounds by the European legislation. The matrix effect was lower than ±30% for all the studied pesticides. The combination of ultra-high performance liquid chromatography and tandem mass spectrometry with this modified quick, easy, cheap, effective, rugged, and safe procedure using Z-Sep allowed a high sample throughput and an efficient cleaning of extracts for the control of these residues in cheeses with a high fat content.

Comparative study for the detection of Egyptian buffalo butter adulteration with vegetable oils using conventional and advanced methods

“…HPLC is one of the analytical techniques used to separate, identify, and quantify the components in a mixture (Hamed, Abdel‐Hamid, Gámiz‐Gracia, García‐Campaña, & Arroyo‐Manzanares, ; Hamed, Moreno‐González, García‐Campaña, & Gámiz‐Gracia, ; Hamed, Moreno‐González, García‐Campaña, & Gámiz‐Gracia, a; Kamal & Karoui, ). For milk and dairy products, HPLC methods could be considered as useful tools to perform several measurements related to the quantification and detection of parameters such as (i) dairy product components; (ii) levels of additive used; and (iii) environmental pollutants and xenobiotics.…”

Multivariate optimization of a new LC–MS/MS method for the determination of 156 pesticide residues in milk and dairy products

“…Several alternative extraction and clean‐up approaches in milk and dairy products have been reported in the literature, i.e. solid‐phase microextraction, 2 dispersive solid‐phase extraction, 3 dispersive liquid–liquid microextraction, 4 QuEChERS (quick, easy, cheap, effective, robust and safe) 5 . The original QuEChERS method developed by Anastassiades et al ., 6 which consists of microextraction with acetonitrile and dispersive solid‐phase extraction, is mainly employed for non‐fatty food samples such as natural agricultural products.…”

Ultra high performance liquid chromatography with tandem mass spectrometry method for determining dinotefuran and its main metabolites in samples of plants, animal‐derived foods, soil, and water

“…Rejczak and Tuzimski and Hamed et al. developed a method for the determination of pesticides in samples of animal origin using Z‐Sep+.…”

“…Promising new sorbents such as zirconia-coated silica (Z-Sep+) enhance sample cleanup for complex matrixes by effectively removing more fat and color from sample extracts than traditional phases for QuEChERS. Rejczak and Tuzimski [16] and Hamed et al [17] developed a method for the determination of pesticides in samples of animal origin using Z-Sep+.…”