Evaluation of a Novel Culture System for Rapid Pathogen Identification and Detection of Cephalosporin Resistance in Neonatal Gram-negative Sepsis at a Tertiary Referral Unit in Harare, Zimbabwe

Chimhini, Gwendoline; Olaru, Ioana D.; Fitzgerald, Felicity; Chisenga, Mutsawashe; Ferreyra, Cecilia; Malou, Nada; Piton, Jérémie; Chimhuya, Simbarashe; Yeung, Shunmay; De, Surya K.; Mujuru, Hilda; Kranzer, Katharina

doi:10.1097/inf.0000000000003155

Cited by 7 publications

(5 citation statements)

References 18 publications

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“…stroke (AIS) is not superior to intensive medical management (IMM) alone [28,29]. By IMM, we mean controlling patients' lipids, blood pressure, and blood glucose; regulating patients' lifestyles; enforcing smoking cessation; and encouraging exercise on the top of antiplatelet therapy [30]. However, even if IMM is strictly implemented in patients with sICAS, their 1-year stroke recurrence and mortality rates are maintained at more than 10% [31].…”

Section: Discussionmentioning

confidence: 99%

Effect of Risk-Focused Diversified Safety Management Mode in Patients with Major Artery Stent Implantation

Shao

Mao

et al. 2022

Emergency Medicine International

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Background. Intracranial atherosclerotic stenosis (ICAS) causes a series of neurological symptoms, such as vertigo, impaired consciousness, limb weakness, ataxia, dysphagia, ocular motility disorders, and visual impairment. With the improvement of people’s living standards, there are higher requirements for nursing care. Nursing, as an indispensable part of medical care, is closely related to achieving the goal of patient’s safety and the overall quality of nurses, quality of care, and nursing management methods. Objective. To explore the effect of risk-centered diversified safety management in patients undergoing aortic stenting. Methods. Eighty patients with cerebral infarction were selected and treated with percutaneous transluminal angioplasty and stent implantation (PTAS). Then they were divided into a control group (40 cases) with routine monitoring and an experimental group (40 cases) with risk-focused intervention of a diversified safety management model according to the mode of care. Patient satisfaction and blood index test results were compared after the intervention. Results. Patients in the experimental group had 6 falls, 3 bed falls, 3 phlebitis, 4 tube slips, and 10 deep vein thrombosis, all significantly fewer than those in the control group. Thirty-eight patients in the experimental group expressed satisfaction with safe management, which was substantially better than the control group ( P < 0.05 ). The levels of tissue plasminogen activator (tPA), plasminogen activator inhibitor-1 (PA1-1), and von Willebrand factor (vWF) in the experimental group were (13.5 ± 1.3) ng/mL, (60.1 ± 9.9) ng/mL, and (2.1 ± 0.2), respectively, which were substantially lower than those in the control group ((14.6 ± 2.4) ng/mL, (64.2 ± 10.7) ng/mL, and (2.8 ± 0.3)), respectively ( P < 0.05 ). Conclusion. The risk-centered diversified safety management model can effectively reduce the probability of adverse events in patients, improve patient satisfaction with nursing services, and promote faster postoperative recovery, which has clinical application value.

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Section: Discussionmentioning

confidence: 99%

Effect of Risk-Focused Diversified Safety Management Mode in Patients with Major Artery Stent Implantation

Shao

Mao

et al. 2022

Emergency Medicine International

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“…The InTray cassettes have already been successfully used in LMICs, targeting uropathogens from urine specimens [19] and ESBL Enterobacterales from blood cultures, where the combination of Colorex Screen and ESBL chromogenic media was able to reduce turnaround time significantly [20]. In this study, we tested the combination of InTray™ MH chocolate and Colorex Screen media for a wider application, in early orientation and diagnosis of sepsis, as a simple alternative to the classic combination of selective and non-selective media.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of InTray Cassettes Directly from Blood Cultures for the Diagnosis of Sepsis in Clinical Bacteriology Laboratories as an Alternative to Classic Culture Media

et al. 2023

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Culture media is fundamental in clinical bacteriology for the detection and isolation of bacterial pathogens. However, in-house media preparation could be challenging in low-resource settings. InTray® cassettes (Biomed Diagnostics) could be a valid alternative as they are compact, ready-to-use media preparations. In this study, we evaluate the use of two InTray media as a subculture alternative for the diagnosis of bloodstream infections: the InTray® Müller-Hinton (MH) chocolate and the InTray® Colorex™ Screen. The InTray MH chocolate was evaluated in 2 steps: firstly, using simulated positive blood cultures (reference evaluation study), and secondly, using positive blood cultures from a routine clinical laboratory (clinical evaluation study). The Colorex Screen was tested using simulated poly-microbial blood cultures. The sensitivity and specificity of the InTray MH chocolate were respectively 99.2% and 90% in the reference evaluation study and 97.1% and 88.2% in the clinical evaluation study. The time to detection (TTD) was ≤20 h in most positive blood cultures (99.8% and 97% in the two studies, respectively). The InTray® MH Chocolate agar showed good performance when used directly from clinical blood cultures for single bacterial infections. However, mixed flora is more challenging to interpret on this media than on Colorex™ Screen, even for an experienced microbiologist.

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“…Pre-prepared, commercially available culture media such as the InTray COLOREX Screen and ESBL agar plates (Biomed Diagnostics) have the potential to aid primary sample processing and transport to a referral laboratory, improve turnaround times for final result reporting to clinicians and aid early detection of ESBL-producing organisms. 13 The InTray media are small, sealable commercially produced agar plates, similar to media used commonly in many microbiology laboratories worldwide, but designed for inoculation in clinics or remote sites prior to transport to testing laboratories. Like other chromogenic media, different bacterial species produce different coloured colonies, allowing rapid presumptive identification.…”

Section: Introductionmentioning

confidence: 99%

“…A recent study in Zimbabwe on the utility of the InTray COLOREX Screen and ESBL plates showed growth was seen on the plates within 5–10 h of incubation, which allowed for rapid identification and a reduction in time of 60% to reporting of results. 13 …”

Section: Introductionmentioning

confidence: 99%

Utility of InTray COLOREX Screen agar and InTray COLOREX ESBL agar for urine culture in the Lao PDR

Roberts

Silisouk

Sengdatka

et al. 2022

JAC-Antimicrobial Resistance

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Background There is a need for simple microbiology diagnostics to enable antimicrobial resistance surveillance in low- and middle-income countries. Objectives To investigate the field utility of InTray COLOREX plates for urine culture and ESBL detection. Methods Clinical urine samples from Mahosot Hospital, Vientiane, Lao PDR were inoculated onto chromogenic media and InTray COLOREX Screen plates between June and August 2020. Urine and isolates from other clinical specimens were inoculated onto COLOREX ESBL plates. A simulated field study investigating the field utility of the InTray COLOREX plates was also completed. Results In total, 355 urine samples were inoculated onto standard chromogenic agar and InTray COLOREX Screen plates, and 154 urine samples and 54 isolates from other clinical specimens on the COLOREX ESBL plates. Growth was similar for the two methods (COLOREX Screen 41%, standard method 38%) with 20% discordant results, mainly due to differences in colony counts or colonial appearance. Contamination occurred in 13% of samples, with the COLOREX Screen plates showing increased contamination rates, potentially due to condensation. ESBL producers were confirmed from 80% of isolates from the COLOREX ESBL plates, and direct plating provided rapid detection of presumptive ESBL producers. Burkholderia pseudomallei also grew well on the ESBL plates, a relevant finding in this melioidosis-endemic area. Conclusions The InTray COLOREX Screen and ESBL plates were simple to use and interpret, permitting rapid detection of uropathogens and ESBLs, and have the potential for easy transport and storage from field sites and use in laboratories with low capacity.

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Evaluation of a Novel Culture System for Rapid Pathogen Identification and Detection of Cephalosporin Resistance in Neonatal Gram-negative Sepsis at a Tertiary Referral Unit in Harare, Zimbabwe

Cited by 7 publications

References 18 publications

Effect of Risk-Focused Diversified Safety Management Mode in Patients with Major Artery Stent Implantation

Effect of Risk-Focused Diversified Safety Management Mode in Patients with Major Artery Stent Implantation

Evaluation of InTray Cassettes Directly from Blood Cultures for the Diagnosis of Sepsis in Clinical Bacteriology Laboratories as an Alternative to Classic Culture Media

Utility of InTray COLOREX Screen agar and InTray COLOREX ESBL agar for urine culture in the Lao PDR

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