Evaluation of a Rapid Bauer-Kirby Antibiotic Susceptibility Determination

Liberman, Daniel F.; Robertson, Richard G.

doi:10.1128/aac.7.3.250

Cited by 17 publications

(11 citation statements)

References 7 publications

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“…Our findings indicate that laboratories without access to automated susceptibility testing instruments can perform DST by traditional disk diffusion technology. Whether early readings from disk susceptibility test plates might be able to provide even more rapid results (6,9,24,30,31) in DST of urine specimens has not been evaluated.…”

Section: Discussionmentioning

confidence: 99%

Direct antimicrobial susceptibility testing for acute urinary tract infections in women

1995

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Despite its theoretical advantages, direct antimicrobial susceptibility testing (DST) of urine specimens remains controversial largely because of concerns regarding its accuracy, particularly with mixed cultures. To evaluate the performance of DST in the setting of acute urinary tract infection (UTI), we performed DST using 25 traditional and contemporary antimicrobial agents on urine specimens from 162 women with suspected acute uncomplicated UTI, and compared these results with the results of standardized disk diffusion susceptibility tests done on the same specimens. Direct tests were interpretable for 129 specimens, i.e., 80% of all specimens and 85% of the 152 specimens that met the culture criteria for UTI. Of the 2,983 individual comparisons between the direct and standard tests, 0.8% represented very major errors, 0.6% represented major errors, 3.1% represented minor errors, and 95.5% were in agreement. Errors were more common in association with older antimicrobial agents and agents with a high prevalence of antimicrobial resistance, non-Escherichia coli strains, low urine bacterial concentrations, sparse or mixed growth in the direct test, and the presence of multiple significant organisms in urine. The urine leukocyte concentration was >15/mm 3 in all subjects and did not differentiate between specimens that gave an interpretable direct test and those that did not. Calculation of the sensitivity of DST in identifying antimicrobial resistance supplemented conventional error rate analysis. We conclude that when used selectively and interpreted carefully, DST of urine specimens offers an efficient, rapid, and accurate method for antimicrobial susceptibility determination for acute UTI, particularly when the urine bacterial concentration is >10 5 CFU/ml.

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Section: Discussionmentioning

confidence: 99%

Direct antimicrobial susceptibility testing for acute urinary tract infections in women

1995

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“…8. 10,11), there has been no evaluation of rapid susceptibility results in which both direct inoculation and early reading were combined. This study was undertaken to test the accuracy of 4-and 6-h readings of disk diffusion tests that were inoculated directly from positive blood cultures.…”

mentioning

confidence: 99%

Rapid antimicrobial susceptibility testing of isolates from blood cultures by direct inoculation and early reading of disk diffusion tests

Coyle¹,

McGonagle²,

Plorde³

et al. 1984

J Clin Microbiol

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Disk diffusion tests, inoculated directly from positive blood cultures, were evaluated for accuracy of reading zone diameters after 4-and 6-h and overnight incubation. In comparisons with results from standard disk diffusion tests, the 4-h results were in agreement for 83% of tests with gram-positive organisms and 64% of tests with gram-negative organisms. When minor discrepancies were ignored, the 4-h readings were in agreement for 98% of the tests with gram-positive organisms and 95% of the tests with gram-negative organisms. After 6 h of incubation, 91% of the tests with gram-positive organisms and 86% of the tests with gram-negative organisms agreed with standard results. The agreement was 99% for tests with both grampositive and gram-negative organisms when minor discrepancies were excluded. Very major discrepancies occurred in two tests (0.1 %) with gram-positive organisms and were not observed in tests with gram-negative organisms. The frequencies of major discrepancies were 3.5% after 4 h, 0.6% after 6 h, and 0.7% after overnight incubation. Ampicillin and cephalothin tests with Escherichia coli and Klebsiella spp. accounted for 81 % of the major discrepancies in tests with gram-negative organisms. Oxacillin tests accounted for more than half of the major discrepancies in tests with staphylococci. The results of this study, which did not include the newer antibiotics, indicate that direct susceptibility tests from blood cultures read after 6 h of incubation are more reliable than 4-h results and produce less than 1 % major errors in comparisons with standard susceptibility tests.

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“…The antibacterial activity of colored-rice extracts was examined using several reliable techniques. It has been noted that Bauer-Kirby disk susceptibility-, MIC-and MBC-based determination are widely used for investigating the antimicrobial susceptibility of various bacteria (Gonzalez et al, 2013;Liberman & Robertson, 1975;Traub, Spohr, & Bauer, 1987;Van Asselt & Van Boven, 1997).…”

Section: Assessment Of Bacterial Survivalmentioning

confidence: 99%

The In-vitro Antibacterial Effect of Colored Rice Crude Extracts against Staphylococcus aureus Associated with Skin and Soft-Tissue Infection

Pumirat¹,

Luplertlop²

2013

JAS

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Due to increases in the resistance of bacteria to the existing antimicrobial agents, plants are being used as alternative sources for the development of safe, effective, and inexpensive new agents to treat and prevent bacterial infections. Recent studies have shown that rice (Oryza sativa L.), an important source of nutrients, consumed by most of the world's population, can suppress some bacterial infections. There are many varieties of rice, e.g. white, brown, black, and red; however, the relationship between rice color and antibacterial properties remains unclear. In the present study, we investigated the antibacterial activity of colored-rice crude extracts from four different types of colored rice (Hom Nil, Neaw Dum, Mun Poo, and Sang Yod) against common bacteria causing skin and soft-tissue infections, such as Staphylococcus aureus, Streptococcus pyogenes, Enterococcus spp., Pseudomonas aeruginosa, and Escherichia coli. The results showed that all colored-rice crude extracts had antibacterial effects against S. aureus; and more notably, crude extracts of differently colored rice restricted diverse antibacterial activities. To our knowledge, this is the first report that provides a basic understanding of the antibacterial properties of colored rice against skin and wound pathogens. An understanding of these properties would be invaluable in the development of alternative, natural, and safe methods of controlling bacterial infections.

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Evaluation of a Rapid Bauer-Kirby Antibiotic Susceptibility Determination

Cited by 17 publications

References 7 publications

Direct antimicrobial susceptibility testing for acute urinary tract infections in women

Direct antimicrobial susceptibility testing for acute urinary tract infections in women

Rapid antimicrobial susceptibility testing of isolates from blood cultures by direct inoculation and early reading of disk diffusion tests

The In-vitro Antibacterial Effect of Colored Rice Crude Extracts against Staphylococcus aureus Associated with Skin and Soft-Tissue Infection

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