Evaluation of a Single-Platform Technology for Lymphocyte Immunophenotyping

Higgins, Jeanette; Hill, Valerie L.; Lau, Karen; Simpson, Virginia Z.; Roayaei, Jean; Klabansky, Rick; Stevens, Randy; Metcalf, Julia A.; Baseler, Michael

doi:10.1128/cvi.00168-07

Cited by 7 publications

(9 citation statements)

References 5 publications

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“…They concluded that the fully automated Beckman Coulter tetraONE system resulted in a small but signiÞ cant improvement in the within laboratory precision of the CD4 and CD8 cell counts and percentages. However a recent study [8] states that they would prefer DPT over SPT for activation markers (DR, CD25 and CD38) of HIV patients.…”

Section: Introductionmentioning

confidence: 99%

Reference ranges for lymphocyte subsets in adults from western India: Influence of sex, age and method of enumeration

Das¹,

Bhanushali²,

Khadapkar³

et al. 2008

Indian J Med Sci

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Section: Introductionmentioning

confidence: 99%

Reference ranges for lymphocyte subsets in adults from western India: Influence of sex, age and method of enumeration

Das¹,

Bhanushali²,

Khadapkar³

et al. 2008

Indian J Med Sci

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“…In this regard, it is important to stress that using a lymphocyte count in a dual platform system for generation of absolute CD41 T lymphocytes will produce highly variable results (28,31). Once these method comparison tools were applied using best practice, did they concur in their selection of suitable CD4 technologies.…”

Section: Discussionmentioning

confidence: 99%

Choosing a new CD4 technology: Can statistical method comparison tools influence the decision?

Scott

Kestens

Pattanapanyasat

et al. 2017

Cytometry Part B Clinical

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“…The evaluation of the absolute counts of T-lymphocyte subsets in the peripheral blood might serve as an important disease course indicator. 7,8 In MSF, such investigation could provide indirect assessment of T-lymphocyte distribution in the perivascular space.…”

Section: ключевые слова: средиземноморская пятнистая лихорадка субпоmentioning

confidence: 99%

Section: Forms With Multiorgan Involvement (Malignant)mentioning

confidence: 99%

“…8 In their study on patients with HIV/AIDS the results of the two methods -DPT and single-platform technology (SPT) showed very good correlation for CD4 and CD8 measurement, though DPT was preferred over SPT for studying of activation markers. 8 STATISTICAL ANALYSIS Statistical analysis was performed using SPSS 15.0.1 software package. Because of an absence of Gaussian distribution of the variables, the differences between two mean values were evaluated for statistical signifi cance using the non-parametric Mann-Whitney U rank-sum test for not normally distributed variables for two groups, and by Kruskal-Wallis nonparametric ANOVA test for three or more groups.…”

Section: Forms With Multiorgan Involvement (Malignant)mentioning

confidence: 99%

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Clinical Investigations. T-Lymphocyte Subset Absolute Counts in the Peripheral Blood of Mediterranean Spotted Fever Patients: Relations to Disease Severity / Абсолютное Количество Субпопуляций Т-Лимфоцитов В Перифери- Ческой Крови Пациентов Со Средиземноморской Пятнистой Лихо- Радкой: Установление Связи С Тяжестью Заболевания

Baltadzhiev¹,

Pavlov²

2015

Folia Medica

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INTRODUCTION: Mediterranean spotted fever (MSF) in Bulgaria is caused by Rickettsia conorii conorii. Aim:This study aims at investigating the absolute counts of T-lymphocyte subsets in the peripheral blood of patients with MSF in order to establish relationships with disease severity. MATERIALS AND METHODS: The absolute counts of T-lymphocyte subsets were tested in the blood of 62 patients in the acute stage of MSF. They were assigned into three age and sex matched groups, based on the severity of disease -with mild, moderate or severe forms. Controls were 32 age and sex matched healthy individuals. The diagnosis was confi rmed by an immunofl uorescence assay. Immunophenotyping was performed using Epics XL-MCL Coulter, USA fl ow-cytometer. RESULTS: The absolute counts of immune competent (CD3 + ) cells, as well as the counts of helper/inducer (CD3 + CD4 + ) and suppressor/ cytotoxic (CD3 + CD8 + ) T-cell subsets decreased in parallel with disease severity. Naïve (CD4 + CD45RA + ) and activated memory (CD4 + CD45RO + ) T-cell subsets were reduced, particularly in severe MSF. Taken as a whole, the counts of activated (CD3 + HLA-DR + ) and that of presenting accessory (CD28 + ) or stimulatory (CD38 + ) molecules Т-cell subsets was increased, but in the fi rst two subsets the trend from mild to severe forms of the disease was descending. CONCLUSION: Reduced T-lymphocyte subset counts are likely related to trans-migration into perivascular infl ammatory foci. The increased number of T-lymphocytes bearing activation molecules refl ects a mobilization of the cell-mediated immune response. An important issue of this study is the possible prognostic value of T-cell subsets counting, predicting the evolution of a clinical condition to clinical forms, according to the disease severity. Т-лимфоцитов (CD4+CD45RA+), как и активированных Т-клеток памяти (CD4+CD45RO+) уменьшается количественно преимущественно при тяжёлых формах СПЛ. В общей сложности абсолютное количество активированных (CD3+HLA-DR+) и экспрессирующих акцессорные (CD28+) или стимуляторные (CD38+) молекулы Т-клеток повышено, но в первых двух субпопуляциях тренд от лёгких к тяжёлым формам заболевания является нисходящим. ЗАКЛЮЧЕНИЕ: Пониженные количества субпопуляций Т-лимфоцитов вероятно связаны с трансмиграцией в очаг воспаления. Повышенное количество Т-лимфоцитов, несущих активационные маркеры отражает мобилизацию клеточно-опосредованного иммунного ответа. Важным выводом исследования является возможное прогнозируемое количество Т-клеточных субпопуляций, предсказывающих трансформацию клинического состояния в клиническую форму в соответствии с тяжестью заболевания. Medica 2015; 57(2): 93-103 Ключевые слова: средиземноморская пятнистая лихорадка, субпопуляции Т-лимфоцитов -абсолютное количество, корреляции с тяжестью заболевания Folia

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Evaluation of a Single-Platform Technology for Lymphocyte Immunophenotyping

Cited by 7 publications

References 5 publications

Reference ranges for lymphocyte subsets in adults from western India: Influence of sex, age and method of enumeration

Reference ranges for lymphocyte subsets in adults from western India: Influence of sex, age and method of enumeration

Choosing a new CD4 technology: Can statistical method comparison tools influence the decision?

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