Mental workload is useful to evaluate the performance of ship bridge teammates: a captain, a duty officer, a helmsman, and a pilot. The physiological indices, heart rate variability (R-R interval), salivary amylase and facial (nasal) temperature, are good indices for ship handling. It evaluated for seafarers on both of sim ulator and real ship; however, we need to evaluate using three indices simultaneously. This paper proposes that three physiological indices do well for evaluating student's mental workload for simulator training.