Evaluation of a Synthetic Oligonucleotide Probe for Diagnosis of Plasmodium falciparum Infections

Mucenski, Cathleen M.; Cross, John H.; Watt, George; Walliker, David; Majam, Olegario R.; Tuazon, Melinda; Quakyi, Isabella A.; Scheibel, L. W.; Trosper, James H.; Buesing, Mary; Ranoa, Catherine P.; Perine, Peter L.; Sangalang, Ruperto; Guerry, Patricia; Szarfman, Ana

doi:10.4269/ajtmh.1986.35.912

Cited by 27 publications

(7 citation statements)

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“…The specificity of the probe was not established. Recently, Mucenski and co-workers reported that, when using an oligonucleotide probe to examine 20 p.1 of blood spotted on nitrocellulose, as few as 50 parasites per ,ul of blood could be detected (87). This matches the sensitivity reported by Franzen et al, but a simpler lysis procedure was used.…”

Section: Probes To Enteroviruses and Other Viral Agentssupporting

confidence: 63%

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Diagnostic deoxyribonucleic acid probes for infectious diseases.

Tenover

1988

Clinical Microbiology Reviews

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Section: Probes To Enteroviruses and Other Viral Agentssupporting

confidence: 63%

“…Finally, researchers have utilized the highly repetitive sequences often found throughout the genomes of higher organisms (60) to develop probes to eucaryotic pathogens such as the malarial parasite Plasmodiumfalciparum (41,81,87) and the filarial worm Brugia malayi (120,121). The process of probe development is summarized in Fig.…”

Section: Downloaded Frommentioning

confidence: 99%

Diagnostic deoxyribonucleic acid probes for infectious diseases.

Tenover

1988

Clinical Microbiology Reviews

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“…The specificity of the probe was not established. Recently, Mucenski and co-workers reported that, when using an oligonucleotide probe to examine 20 p.1 of blood spotted on nitrocellulose, as few as 50 parasites per ,ul of blood could be detected (87 (120). The authors state that the assay will detect the presence of even a single filarial worm present in a mosquito.…”

Section: Probes To Enteroviruses and Other Viral Agentsmentioning

confidence: 99%

Section: Definitionsmentioning

confidence: 99%

Diagnostic deoxyribonucleic acid probes for infectious diseases

Tenover

1988

Clin Microbiol Rev

239

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Virtually all microorganisms contain some unique nucleotide sequences which can be the target of deoxyribonucleic acid probes. Probes have been used successfully to identify a wide variety of pathogens from the simple ribonucleic acid-containing polioviruses to the complex filarial worms Brugia malayi. Probe technology offers the clinical laboratory the potential both to extend the types of pathogens that can be readily identified and to reduce significantly the time associated with the identification of fastidious microorganisms. Over a dozen commercially prepared deoxyribonucleic acid probe tests are now available. This article explores the development of deoxyribonucleic acid probe tests and reviews the sensitivity, specificity, and predictive values of many of the diagnostic probes developed during the last several years. Prospects for newer, more sensitive detection systems for the products of hybridization reactions are also reviewed.

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“…The gel was stained with ethidium bromide and visualized under UV before the material was transferred onto a nylon membrane by Southern blotting. The membrane was hybridized with y-32P end-labeled 21-mer synthetic oligonucleotide, which is known to be repetitive sequence in P. falciparum genome (20), at 42 C under stringent washing conditions. The sequence of the oligomer used to probe the membrane was as follows:…”

Section: Methodsmentioning

confidence: 99%

Cure With Cisplatin (Ii) of Murine Malaria Infection and in Vitro Inhibition of a Chloroquine-Resistant Plasmodium Falciparum Isolate

Nair

Bhasin

1994

JJMSB

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SUMMARY:Antiplasmodium properties of cisplatin [cis-platinum (II) diammine dichloride], a neoplastic drug, have been assessed in in vivo and in vitro model systems of malarial parasite. A well-tolerated dose of 6 mg/kg body weight of the compound cured the mice infected with Plasmodium berghei and the amount of cisplatin required for in vitro inhibition (IC50) of a chloroquineresistant Plasmodium falciparum isolate was smaller than either chloroquine or quinine. The minimum inhibitory concentration (MIC) needed to prevent the in vitro multiplication of asexual blood parasites was 30 ng/ml. Late ring and trophozoite stages of the erythrocytic cycle were the most susceptible, whereas schizont and early ring stages were the least sensitive to the toxic effect of cisplatin. Multiple smaller doses were more effective in curing malaria in mice than a single large dose. In a few of the mice treated with a single intraperitoneal large dose of 6 mg/kg body weight, there was a delay in appearance of parasitemia but most of them recovered completely but slowly. This compound exerts its toxicity mainly by randomly damaging and cross-linking DNA strands as shown by Southern hybridization with a synthetic oligonucleotide probe, which is a repeat sequence in the falciparum genome. The report clearly demonstrates the antimalarial potentials of this compound and suggests a closer evaluation of this and other related compounds, specially in combination with antimalarial drugs to probe their synergistic properties.241

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Evaluation of a Synthetic Oligonucleotide Probe for Diagnosis of Plasmodium falciparum Infections

Cited by 27 publications

References 0 publications

Diagnostic deoxyribonucleic acid probes for infectious diseases.

Diagnostic deoxyribonucleic acid probes for infectious diseases.

Diagnostic deoxyribonucleic acid probes for infectious diseases

Cure With Cisplatin (Ii) of Murine Malaria Infection and in Vitro Inhibition of a Chloroquine-Resistant Plasmodium Falciparum Isolate

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