Evaluation of a System Using Oral Mucosal Transudate for HIV-1 Antibody Screening and Confirmatory Testing

Gallo, D

doi:10.1001/jama.1997.03540270080030

Cited by 127 publications

(77 citation statements)

References 25 publications

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“…[12][13][14] As with all EIA techniques, there is a hazard of false-positive results from lipemic samples. 9 Past research has demonstrated that false-positive serologic test results have occurred with cross-reactive antibodies or interfering substances in serum.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of rapid diagnostic tests for the detection of human immunodeficiency virus types 1 and 2, hepatitis B surface antigen, and syphilis in Ho Chi Minh City, Vietnam.

Lien¹,

Tien²,

Chanpong³

et al. 2000

The American Journal of Tropical Medicine and Hygiene

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Abstract. An evaluation of three new rapid diagnostic test kits for human immunodeficiency virus types 1 and 2 (HIV-1/2), hepatitis B surface antigen (HBsAg), and syphilis involved a two-phase comparison of rapid diagnostic assays using prospectively collected from hospitals and clinics in Ho Chi Minh City, Vietnam. After specificity and sensitivity testing, three new rapid diagnostic test kits were tested in parallel with six commonly used diagnostic test kits. The Determine HIV-1/2 test had fewer indeterminate or equivocal results than the Capillus HIV-1/HIV-2 or HIV Blot 2.2 tests. However, the Determine HIV-1/2 test yielded one false-positive result when compared with the Serodia HIV, HIV Blot 2.2, and microparticle enzyme immunoassay (IMx) HIV tests. The Serodia HBsAg test yielded more false-negative results when compared with the Determine HBsAg diagnostic test kit. The results of the syphilis diagnostic tests evaluated in this clinical trial consistently agreed with those of the rapid plasma reagin test for syphilis. The Determine Syphilis Treponema pallidum (TP) test had three false-positive results compared with the Serodia TP and the Serodia TP•particle agglutination (PA) tests, which had two false-positive results that were confirmed as negative by an ELISA. Application of these serologic tests within this comparative evaluation framework, using the World Health Organization alternative testing strategies, proved to be an effective way to determine serostatus related to HIV, hepatitis B, and syphilis.In many developing areas worldwide, field and clinic laboratory capabilities may be insufficient for the detection of infectious agents for definitive clinical diagnostic purposes. The absence of simple, rapid diagnostic testing methods for sexually transmitted diseases (STDs) and hepatitis has significantly hampered public health efforts to retard the spread of these diseases. The inability to provide tests for quick recognition of human immunodeficiency virus (HIV), hepatitis B, and syphilis has allowed infected individuals to unknowingly spread the disease through sexual contacts, blood donations, and intravenous needle sharing. In cities throughout Asia, current laboratory evaluation of blood specimens may preclude case follow-up and counseling due to a long time lag between initial sample collection and conventional test completion. High-risk populations typically seek treatment during clinic visits in association with acute episodes and are not likely to return a second time for test results.Diagnostic technology is adapting itself for application in developing countries. Advancements in the laboratory diagnosis of HIV/acquired immunodeficiency syndrome (AIDS), hepatitis B, and syphilis have considered the following conditions, including: 1) speed of results; 2) test validity and accuracy; 3) minimal specimen requirement; 4) variable type of specimen, including whole blood; 5) ease of test kit use, with few requirements for specialized laboratory equipment; and 6) stable reagents, requiring no refri...

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Section: Discussionmentioning

confidence: 99%

Evaluation of rapid diagnostic tests for the detection of human immunodeficiency virus types 1 and 2, hepatitis B surface antigen, and syphilis in Ho Chi Minh City, Vietnam.

Lien¹,

Tien²,

Chanpong³

et al. 2000

The American Journal of Tropical Medicine and Hygiene

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“…At the study visit, all NEP participants presented their study card (received from the NEP coordinator), received urine toxicological testing (to confirm heroin or cocaine use), underwent HIV-testing using Orasure, saliva-based testing, and received a 45-minute, face-to-face interview with study staff (18). The questionnaire included sections on demographics, health service use, drug use, and HIV risk behaviors (described below).…”

Section: Methodsmentioning

confidence: 99%

“…The majority of studies that have assessed drug and sex HIV-risk behaviors have focused on out-of-treatment samples of IDUs, recruited from the street or needle-exchange programs (1,6,8,10,11,13,14,(16)(17)(18). Some have assessed drug users' HIV risk behaviors in inpatient treatment, primary care clinics, methadone maintenance programs, and while incarcerated (9,12,15,19).…”

Section: Introductionmentioning

confidence: 99%

Intravenous Drug Users' HIV‐Risk Behaviors with Primary/Other Partners

Rosengard

Anderson

Stein

2004

The American Journal of Drug and Alcohol Abuse

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The objective of this study was to determine how injection drug users' (IDUs) HIV-risk behavior differs with primary and other sex partners. Interviews were conducted with injection drug users from a needle exchange program (n = 243). Those with one sexual partner were more likely to report never using condoms with primary partners than were those with more than one partner (74% vs. 54%, p < 0.001). Those with more than one partner differed, between primary and other sexual partners, in their disclosure of HIV and IDU status, condom use, and drug use in combination with sex. Primary sexual partners of IDUs are placed at risk from IDUs' risk behavior with other sexual partners. Those planning HIV-risk reduction interventions for IDUs should consider risk behavior with primary partners separately from behavior with other partners. Evaluation of intervention effects should use partner-specific assessments of risk behavior.

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“…These studies have evaluated various outcomes, such as diagnostic accuracy, client preference, acceptability, uptake and cost-and time-effectiveness [13][14][15][16][17]. It is now an established fact that diagnostic accuracy of oral rapid point-of-care tests is high and that their test results are consistent across developed and developing countries [7,13,18].…”

Section: For Reprint Orders Please Contact: Reprints@future-drugscommentioning

confidence: 99%

Oral fluid-based rapid HIV testing: issues, challenges and research directions

Pai¹

2007

Expert Review of Molecular Diagnostics

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Evaluation of a System Using Oral Mucosal Transudate for HIV-1 Antibody Screening and Confirmatory Testing

Cited by 127 publications

References 25 publications

Evaluation of rapid diagnostic tests for the detection of human immunodeficiency virus types 1 and 2, hepatitis B surface antigen, and syphilis in Ho Chi Minh City, Vietnam.

Evaluation of rapid diagnostic tests for the detection of human immunodeficiency virus types 1 and 2, hepatitis B surface antigen, and syphilis in Ho Chi Minh City, Vietnam.

Intravenous Drug Users' HIV‐Risk Behaviors with Primary/Other Partners

Oral fluid-based rapid HIV testing: issues, challenges and research directions

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