Evaluation of Activity of Phospholipids in Blood Coagulation in Vitro

Abstract

Summary1. The effects of phospholipids, solubilized by different methods in different clotting tests are presented.2. Complete fatty acid analysis of a phosphatidylserine fraction from beef brain is given.3. Data relating electric charge of phospholipid particles to activity in clotting are presented.4. A critical discussion of the factors which influence the activity of phospholipids in blood clotting terminates the paper.

Characteristics of phospholipids associated with fibrins in partial thromboplastin test.

“…In the process, several workers have maintained that phosphatidylethanolamine is a procoagulant phospholipid (Rouser et al 1958;Wallach et al 1959;, whereas others have ascribed this catalytic action to phosphatidylserine (Troup and Reed 1958;Silver et al 1963;Slater et al 1980). On the other hand, several groups have recognized that a mixture of at least two phospholipids including phosphatidylethanolamine or phosphatidylserine produces the highest clot promoting activity (Troup et al 1960;Hecht and Slotta 1962).…”

“…A number of studies have been made on the relationship between individual lipid classes and thromboplastic activity to determine the lipid component responsible for the coagulation process (Rouser et al 1958;Daemen et al 1965;Slater et al 1980). As a result, it has been reported that among the lipid classes, only phospholipids, particularly phosphatidylethanolamine (Rouser et al 1958;Wallach et al 1959;Turner et al 1963) and phosphatidylserine (Troup and Reed 1958;Silver et al 1963;Slater et al 1980), have thromboplastic activity which may be ascribed to their negative charge on the micelle surface (Wallach et al 1959;Bangham 1961).…”

Platelet Adhesiveness and Thrombosis in Rabbits on an Atherogenic Diet

“…Serum cholesterol was determined by the Technicon method [25]. Phosphatidyl serine obtained from the Nutritional Biochemical Corporation was pre pared for infusion by the paste method of Silver et al [24]. In this method an appropriate amount of the phospholipid to give a final dose of 35 mg/kg body weight was suspended in 1.0 ml of dilute dextrose alkali solution, pi I 9.0.…”

“…Silver [24] reported on the various events which take place in the Chandler loop after recalcification of the PRP. In the present studies with rabbit PRP, a few small particles barely visible to M e a n ± S .…”

Activation of histamine secretion from rat mast cells by aqueous dispersions of phosphatidylserine

“…Bovine brain PS is a heterogeneous mixture of l,2-diacyl-i«-3glycerophosphoserines; however, greater than 80% of the esterified fatty acids are either stearic or oleic acid(Silver et al, 1963).…”