Evaluation of acute and sub-chronic toxicity of bitter melon seed extract in Wistar rats

Chung, Wan-Yu; Jadhav, Sudhakar; Hsu, Pang-Kuei; Kuan, Chen-Meng

doi:10.1016/j.toxrep.2022.04.024

Cited by 10 publications

(2 citation statements)

References 35 publications

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“…The results obtained fit with the non-hepatotoxic effect previously mentioned. The results were identical to those reported by Wan-yu and collaborators [27] who found no significant difference in serum bilirubin activity when administering aqueous seed-extract of Momordica charantia through sub-acute per os treatment.…”

Section: Discussionsupporting

confidence: 89%

Evaluation of Acute and Sub-Acute Oral Toxicities of <i>Momordica foetida</i> Schumach. (Cucurbitaceae) Leaves Methanol Extract in Wistar Rats

Pouaha¹,

Lienou²,

Dongmo³

et al. 2023

JBM

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Momordica foetida is a plant widely used in tropical Africa to manage gastroenteric diseases. Previous studies demonstrated interesting antibacterial activity against human pathogenic bacteria. However, the security or toxicity of methanol leaf extract has not been determined yet. The aim of this study was to evaluate the acute and sub-acute toxicity of the leaf extract of Momordica foetida. In the acute toxicity study, a single oral dose of 5000 mg/kg body weight was administered to rats which were observed for 14 days in order to identify signs of toxicity or death. In the sub-acute toxicity, the animals were treated with 250, 500 and 1000 mg/kg of the extract for 28 consecutive days. Body weights and behavior were noted throughout the experiment. Upon treatment, blood and urine were collected for hematological and biochemical analysis. Liver, lungs, heart, kidneys, testes and ovaries were analyzed for relative weights and histopathology. The acute toxicity study of M. foetida leaf extract revealed no signs of toxicity related to the treatment, indicating that the median-lethal-dose (LD50) value is greater than 5000 mg/Kg of body weight. In the sub-acute toxicity assay, the extract did not affect the general behavior of animals, meanwhile, it led to a significant increase in the levels of red blood cells, platelets, hemoglobin, granulocytes and Mid-Cells (MIDs). Biochemical parameters showed an increase in total cholesterol, HDL cholesterol, serum urea, serum and urinary glucose and a decrease in urinary proteins, serum creatinine, urinary urea levels, serum activities of AST, ALT and proteins levels, as well as increases in lung, spleen and ovaries relative weight were no-

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Section: Discussionsupporting

confidence: 89%

Evaluation of Acute and Sub-Acute Oral Toxicities of <i>Momordica foetida</i> Schumach. (Cucurbitaceae) Leaves Methanol Extract in Wistar Rats

Pouaha¹,

Lienou²,

Dongmo³

et al. 2023

JBM

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show abstract

“…In the hematological examination, RBC, and HCT seemed to be increased and PLT, MCHC, and MCH appeared to be decreased in level at higher concentration. But there is no concomitant effect on biochemical parameters, signifying no serious issues (Chung et al, 2022). Monitoring urine output, proteins, and glucose levels can help determine renal toxicity (Patel et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Newly synthesized acridone derivatives targeting lung cancer: A toxicity and xenograft model study

Bhusare,

Yadav,

Nandave

et al. 2024

Drug Development Research

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AKT is one of the overexpressed targets in nonsmall cell lung cancer (NSCLC) and plays an important role in its progression and offers an attractive target for the therapy. The PI3K/AKT/mTOR pathway is upregulated in NSCLC. Acridone is an important heterocycle compound which treats cancer through various mechanisms including AKT as a target. In the present work, the study was designed to evaluate the safety profile of three acridone derivatives (AC‐2, AC‐7, and AC‐26) by acute and repeated dose oral toxicity. In addition to this, we also checked the pAKT overexpression and its control by these derivatives in tumor xenograft model. The results from acute and repeated dose toxicity showed these compounds to be highly safe and free from any toxicity, mortality, or significant alteration in body weight, food, and water intake in the rats. In the repeated dose toxicity, compounds showed negligible variations in a few hematological parameters at 400 mg/kg. The histopathology, biochemical, and urine parameters remained unchanged. The xenograft model study demonstrated AC‐2 to be inhibiting HOP‐62 induced tumor via reduction in p‐AKT1 (Ser473) expression significantly. In immunofluorescence staining AC‐2 treated tissue section showed 2.5 fold reduction in the expression of p‐AKT1 (Ser473). Histopathology studies showed the destruction of tumor cells with increased necrosis after treatment. The study concluded that AC‐2 causes cell necrosis in tumor cells via blocking the p‐AKT1 expression. The findings may provide a strong basis for further clinical applications of acridone derivatives in NSCLC.

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Toxicity Potential of Nutraceuticals

Gupta,

Doss

2024

Methods in Molecular Biology

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Evaluation of acute and sub-chronic toxicity of bitter melon seed extract in Wistar rats

Cited by 10 publications

References 35 publications

Evaluation of Acute and Sub-Acute Oral Toxicities of <i>Momordica foetida</i> Schumach. (Cucurbitaceae) Leaves Methanol Extract in Wistar Rats

Evaluation of Acute and Sub-Acute Oral Toxicities of <i>Momordica foetida</i> Schumach. (Cucurbitaceae) Leaves Methanol Extract in Wistar Rats

Newly synthesized acridone derivatives targeting lung cancer: A toxicity and xenograft model study

Toxicity Potential of Nutraceuticals

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Evaluation of acute and sub-chronic toxicity of bitter melon seed extract in Wistar rats

Cited by 10 publications

References 35 publications

Evaluation of Acute and Sub-Acute Oral Toxicities of &lt;i&gt;Momordica foetida&lt;/i&gt; Schumach. (Cucurbitaceae) Leaves Methanol Extract in Wistar Rats

Evaluation of Acute and Sub-Acute Oral Toxicities of &lt;i&gt;Momordica foetida&lt;/i&gt; Schumach. (Cucurbitaceae) Leaves Methanol Extract in Wistar Rats

Newly synthesized acridone derivatives targeting lung cancer: A toxicity and xenograft model study

Toxicity Potential of Nutraceuticals

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Product

Resources

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Evaluation of Acute and Sub-Acute Oral Toxicities of <i>Momordica foetida</i> Schumach. (Cucurbitaceae) Leaves Methanol Extract in Wistar Rats

Evaluation of Acute and Sub-Acute Oral Toxicities of <i>Momordica foetida</i> Schumach. (Cucurbitaceae) Leaves Methanol Extract in Wistar Rats