Evaluation Of An Additive Solution For Preservation Of Canine Red Blood Cells

Wardrop, K. Jane; Owen, Tina; Meyers, Kenneth M.

doi:10.1111/j.1939-1676.1994.tb03228.x

Cited by 43 publications

(46 citation statements)

References 26 publications

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“…The mean PTV of canine red blood cells stored in Nutricel for 35 days was 85% with 5'Cr and 90% with 99"T~/5'Cr. This was significantly higher than the mean PTVs of 38% and 36% for the CPDA-1 stored cells as assessed with 5 One unit (450 mL) of blood was collected from each dog and placed into one of two storage solutions. The storage solutions were assigned randomly to samples obtained from study dogs.…”

mentioning

confidence: 69%

Evaluation of Canine Red Blood Cells Stored in a Saline, Adenine, and Glucose Solution for 35 Days

Wardrop

Tucker²,

Mugnai³

1997

Veterinary Internal Medicne

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An additive solution for the storage of red blood cells was evaluated for use in dogs. Blood collected from 6 dogs was processed into packed red blood cells and stored for 35 days in the additive solution Nutricel (Miles, Inc, Pharmaceutical Division, West Haven, CT). Packed red blood cells stored in citrate-phosphate-dextrose-adenine (CPDA-1; Fenwal Laboratories, Baxter Health Care Corp, Deerfield, IL) also were evaluated for comparison. Red blood cell 2,3-diphosphoglycerate (2,3-DPG) concentration, adenosine triphosphate (ATP) concentration, percentage hemolysis, and pH were determined. The red blood cell post-transfusion viability (PTV) after 35 days of storage was assessed with both single-labeled chromium 51 (5'Cr) and double-labeled technetium 99m/chromium 51 (99"T~/5'Cr) techniques. Mean ATP concentration and percentage hemolysis of the cells stored in Nutricel were 1.1 pmol/g hemoglobin (Hb) and 0.28% respectively and did not differ significantly (P < .05) from the he use of packed red blood cells from dogs for transfu-

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confidence: 69%

Evaluation of Canine Red Blood Cells Stored in a Saline, Adenine, and Glucose Solution for 35 Days

Wardrop

Tucker²,

Mugnai³

1997

Veterinary Internal Medicne

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“…Although samples of pRBCs were evenly distributed, resultant cellular sedimentation might have caused an increase in RBC, Hct and Hb values (Wardrop et al 1994a;Mudge et al 2004). Furthermore, the increase in Hct values was directly correlated with Table 1.…”

Section: Discussionmentioning

confidence: 92%

Freezing and storage of leukodepleted erythrocyte suspensions

Bala¹,

Eraslan²,

Akyazı³

et al. 2016

Vet. Med. - Czech

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Studies on the frozen storage of human blood products have benefited veterinary transfusion medicine in recent years, but the long-term cryopreservation of canine red blood cells (RBCs) has not yet been thoroughly investigated. Further, no studies are available with respect to the frozen storage of leukocyte-depleted canine red blood cells (LD-RBCs). The objective of the current study was to investigate time-dependent effects of long-term frozen storage on leukocyte-depleted canine RBCs. Twelve healthy adult dogs meeting the criteria for blood transfusion were used in the study. Whole blood samples (450 ± 45 ml) collected from each dog were centrifuged for 5 min at 22 °C and 4200 × g in a cryogenic microcentrifuge and concentrated RBC (pRBC) suspensions were obtained. Leukocyte depletion was achieved by filtration (2.6 log 10 ). Then, the filtrated samples were prewashed three times in 0.9% NaCl solution and were allocated into three subgroups to be evaluated at three different time points (Day 0, Month 4 and Month 6). The samples for cryopreservation were subjected to glycerolisation and then stored at -80 °C for 4-and 6-month periods. At the end of this period pRBC units were thawed by manual agitation in a water bath maintained at 36-38 °C, centrifuged and then washed in a consecutive series of 12%, 1.6% and 0.9% of NaCl + 0.2 dextrose solutions. 2,3-Diphosphoglycerate (2,3-DPG), adenosine triphosphate (ATP), supernatant haemoglobin (SupHb), sodium (Na + ) and potassium (K + ) levels, residual glycerol concentrations and haemograms of thawed and deglycerolised pRBC samples were evaluated together with those of Day 0. Sterility tests were performed on all samples for bacterial contamination. No statistically significant differences were noted except for Hct and SupHb levels. No bacterial contamination was noted in any of the samples on the basis of sterility tests. It was found that the described glycerolisation procedure could be a method of choice in the cryopreservation of leukocyte-depleted pRBCs (LD-pRBCs) since no negative effect was observed on the quality of the products and long-term frozen storage did not cause RBC destruction.

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“…A previous study, which compared biochemical alterations in rat and human packed RBC (pRBC) stored in CPDA‐1 for 29 days, reported a faster decrease in the pH mean levels, lowering from 7.04 at T0 to 6.64 at T29 in rats and from 7.14 at T0 to 6.50 at T29 in humans. Studies on canine pRBC CPDA‐1 indicate a lower initial pH (between 6.90 and 6.97) at T0 and a significant drop by T35 (between 6.47 and 6.50). One study on canine pRBC units with other storage solutions showed similar trends as those documented in our study (pH range 7.22–7.30 at T1 and 6.99‐7.07 at T31) .…”

Section: Discussionmentioning

confidence: 99%

In vitro assessment of quality of citrate‐phosphate‐dextrose‐adenine‐1 preserved feline blood collected by a commercial closed system

Crestani

Stefani

Carminato

et al. 2018

Veterinary Internal Medicne

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BackgroundOptimal procedure for storage of feline blood is needed. Open‐collection systems have been employed in feline medicine, thus limiting the possibility for storage.ObjectivesTo evaluate indicators of quality of feline blood stored for 35 days at +4°C in a closed‐collection system specifically designed for cats.AnimalsEight healthy adult European domestic shorthair cats with a weight of 5‐6.8 kg.MethodsThis is a case series study. A bacteriological test, CBC, blood smear, pH, osmotic fragility, 2,3‐diphosphoglycerate (2,3‐DPG), and adenosine triphosphate (ATP) measurement were performed weekly on whole blood (WB) units from day 1 to day 35 after donation. The hemolysis index, lactate and potassium concentrations, prothrombin time (PT), activated partial thromboplastin time (aPTT), and fibrinogen were measured on plasma aliquots.ResultsOne out of eight blood units (BUs) had bacterial growth (Serratia marcescens) at day 35. No significant differences were found regarding CBC, morphology, pH, and osmotic fragility. Despite high inter‐individual variability and low starting levels, significant decreases in the mean concentrations of 2,3‐DPG (T0 1.99 mmol/g Hb, SD 0.52, T35 1.25 mmol/g Hb, SD 1.43; P = .003) and ATP (T0 1.45 mmol/g Hb, SD 0.71, T35 0.62 mmol/g Hb, SD 0.51; P < .001) were detected during the study, as opposed to an increase in hemolysis (T0 0.11 mmol/L, SD 0.07, T35 0.84 mmol/L, SD 0.19; P < .001), lactate (T0 3.30 mmol/L, SD 0.86, T35 13.36 mmol/L, SD 2.90; P < .001), and potassium (T0 3.10 mmol/L, SD 0.21, T35 4.12 mmol/L, SD 0.35; P < .001) concentrations.Conclusions and Clinical ImportanceThe commercial BU kit is appropriate for blood collection and conservation of WB in cats. The maintenance of WB quality indicators during storage is essential for future improvements of feline transfusion medicine.

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Evaluation Of An Additive Solution For Preservation Of Canine Red Blood Cells

Cited by 43 publications

References 26 publications

Evaluation of Canine Red Blood Cells Stored in a Saline, Adenine, and Glucose Solution for 35 Days

Evaluation of Canine Red Blood Cells Stored in a Saline, Adenine, and Glucose Solution for 35 Days

Freezing and storage of leukodepleted erythrocyte suspensions

In vitro assessment of quality of citrate‐phosphate‐dextrose‐adenine‐1 preserved feline blood collected by a commercial closed system

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