Evaluation of an alternative indirect-ELISA test using in vitro-propagated Trypanosoma brucei brucei whole cell lysate as antigen for the detection of anti-Trypanosoma evansi IgG in Colombian livestock

Jaimes-Dueñez, Jeiczon; Zapata-Zapata, Carolina; Triana-Chávez, Omar; Mejía-Jaramillo, Ana María

doi:10.1016/j.prevetmed.2019.104712

Cited by 7 publications

(2 citation statements)

References 38 publications

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“…In Colombia, the prevalence of T. evansi infection has been reported in wild and domestic animals (Jaimes-Dueñez et al, 2017a;Jaimes-Dueñez et al, 2018;Jaimes-Dueñez et al, 2019;Morales et al, 1976); however, the epidemiology of this parasite in Colombian dogs is little known, with few case reports suggesting a high susceptibility of the dogs to development fatal cases (Correa-Salgado et al, 2010;Jaimes-Dueñez et al, 2017b). Herein, we addressed a T. evansi epidemiological surveillance in dogs from MAB, showing a considerable prevalence of infection and animals that can keep chronic infections without complicated clinical signs (Fig.…”

Section: Discussionmentioning

confidence: 99%

Understanding the Epidemiology of Trypanosoma Evansi Infection in Dogs (Canis lupus familiaris) from Urban Areas of Colombia

Jaimes-Dueñez¹,

Jiménez-Leaño²,

Montenegro-Ayala³

et al. 2022

American Journal of Animal and Veterinary Sciences

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Trypanosoma evansi is a protozoan parasite that is the causative agent of the animal disease known as "surra", which affects a wide variety of wild and domestic mammals including humans. This study aimed to evaluate the molecular point prevalence and clinic-epidemiological traits of T. evansi infection in dogs from municipalities of the Metropolitan Area of Bucaramanga (MAB), Santander, Colombia. From 2019 and 2020, a crosssectional study in four municipalities of MAB was carried out to evaluate the above features in 213 healthy dogs from these cities. Molecular analysis using the primer ESAG/6 and ESAG/7, showed a T. evansi frequency of 1.8% (CI 95% = 0.04-3.68%), while Woo test didn't detect positive animals. A high prevalence of infection was detected in Piedecuesta (10.53%) and Bucaramanga (1.65%), apparently negative animals were detected in Giron and Florida Blanca. Clinical characteristics revealed that all positive animals (4/4) showed low Mean Corpuscular Hemoglobin (MCH) and (3/4) low Mean Corpuscular Volume (MCV), and one animal had a moderate fever with prolonged capillary refill time. The epidemiological characteristics showed that all animals received mixed food, (3/4) came from low socioeconomic areas, and (2/4) from neighborhoods without weekly garbage collection. In conclusion, molecular methods detected a considerable prevalence of animals chronically infected by T. evansi in MAB, with more frequency in neighborhoods of a low socio-economic level where animals have more risk of oral transmission. These results should be considered during urban control programs of surra in MAB.

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Section: Discussionmentioning

confidence: 99%

Understanding the Epidemiology of Trypanosoma Evansi Infection in Dogs (Canis lupus familiaris) from Urban Areas of Colombia

Jaimes-Dueñez¹,

Jiménez-Leaño²,

Montenegro-Ayala³

et al. 2022

American Journal of Animal and Veterinary Sciences

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“…The antibody ELISA for IgG detection meets this need. Depending on the Trypanosoma species, a set of one to three ELISAs can be used, including an ELISA for T. vivax , one for T. congolense savannah and one for T. evansi or T. brucei brucei , the results being remarkably similar whichever of the two latter antigens is used [ 17 , 100 ]. When efficient control methods are applied, such serological follow-ups will help to demonstrate a decreasing prevalence.…”

Section: Diagnostic Tools Available and Needed In Line With Various E...mentioning

confidence: 99%

Diagnosis of animal trypanosomoses: proper use of current tools and future prospects

et al. 2022

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Reliable diagnostic tools are needed to choose the appropriate treatment and proper control measures for animal trypanosomoses, some of which are pathogenic. Trypanosoma cruzi, for example, is responsible for Chagas disease in Latin America. Similarly, pathogenic animal trypanosomoses of African origin (ATAO), including a variety of Trypanosoma species and subspecies, are currently found in Africa, Latin America and Asia. ATAO limit global livestock productivity and impact food security and the welfare of domestic animals. This review focusses on implementing previously reviewed diagnostic methods, in a complex epizootiological scenario, by critically assessing diagnostic results at the individual or herd level. In most cases, a single diagnostic method applied at a given time does not unequivocally identify the various parasitological and disease statuses of a host. These include “non-infected”, “asymptomatic carrier”, “sick infected”, “cured/not cured” and/or “multi-infected”. The diversity of hosts affected by these animal trypanosomoses and their vectors (or other routes of transmission) is such that integrative, diachronic approaches are needed that combine: (i) parasite detection, (ii) DNA, RNA or antigen detection and (iii) antibody detection, along with epizootiological information. The specificity of antibody detection tests is restricted to the genus or subgenus due to cross-reactivity with other Trypanosoma spp. and Trypanosomatidae, but sensitivity is high. The DNA-based methods implemented over the last three decades have yielded higher specificity and sensitivity for active infection detection in hosts and vectors. However, no single diagnostic method can detect all active infections and/or trypanosome species or subspecies. The proposed integrative approach will improve the prevention, surveillance and monitoring of animal trypanosomoses with the available diagnostic tools. However, further developments are required to address specific gaps in diagnostic methods and the sustainable control or elimination of these diseases. Graphical Abstract

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Isolation and in vitro cultivation of Trypanosoma evansi Thai strains

Kamyingkird

Chalermwong

Inpankaew

et al. 2022

Experimental Parasitology

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Evaluation of an alternative indirect-ELISA test using in vitro-propagated Trypanosoma brucei brucei whole cell lysate as antigen for the detection of anti-Trypanosoma evansi IgG in Colombian livestock

Cited by 7 publications

References 38 publications

Understanding the Epidemiology of Trypanosoma Evansi Infection in Dogs (Canis lupus familiaris) from Urban Areas of Colombia

Understanding the Epidemiology of Trypanosoma Evansi Infection in Dogs (Canis lupus familiaris) from Urban Areas of Colombia

Diagnosis of animal trypanosomoses: proper use of current tools and future prospects

Isolation and in vitro cultivation of Trypanosoma evansi Thai strains

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