Evaluation of an enzyme-linked immunosorbent assay for detection of antibodies to Junin virus in rodents

Morales, María Alejandra; Calderón, Gladys; Riera, Laura; Ambrosio, Ana María; Enría, Delia; Sabattini, Marta S.

doi:10.1016/s0166-0934(01)00452-9

Cited by 15 publications

(9 citation statements)

References 11 publications

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“…The application of reverse transcriptase PCR (RT-PCR) and TaqMan PCR for detection of the JUNV, MACV, and GTOV genomes has been reported (1,11,12,27). Serological diagnosis is also useful for the diagnosis of AHF, especially in patients in the convalescent phase (7,17,19,20,26).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Monoclonal Antibodies to Junin Virus Nucleocapsid Protein and Application to the Diagnosis of Hemorrhagic Fever Caused by South American Arenaviruses

Nakauchi

Fukushi

Saijo

et al. 2009

Clin Vaccine Immunol

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Junin virus (JUNV), Machupo virus, Guanarito virus, Sabia virus, and Chapare virus are members of NewWorld arenavirus clade B and are the etiological agents of viral hemorrhagic fevers that occur in South America. In this study, we produced three monoclonal antibodies (MAbs) to the recombinant nucleocapsid protein of JUNV, designated C6-9, C11-12, and E4-2. The specificity of these MAbs was examined by enzymelinked immunosorbent assay (ELISA), indirect immunofluorescence assay, and an epitope-mapping method. Using these MAbs, we developed antigen (Ag) capture ELISA systems. We showed that by using MAb C6-9, JUNV Ag was specifically detected. On the other hand, by using MAb C11-12 or E-4-2, the Ags of all human pathogenic South American arenaviruses were detected. The combined use of these Ag capture ELISA systems in the present study may be useful for the diagnosis of acute-phase viral hemorrhagic fever due to infection by a South American arenavirus.

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Section: Discussionmentioning

confidence: 99%

Characterization of Monoclonal Antibodies to Junin Virus Nucleocapsid Protein and Application to the Diagnosis of Hemorrhagic Fever Caused by South American Arenaviruses

Nakauchi

Fukushi

Saijo

et al. 2009

Clin Vaccine Immunol

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“…For the diagnosis of Lassa fever, ELISA assays have recently proved as reliable as indirect fluorescent antibody testing, the previous standard method of viral identification [57]. Junin virus, the cause of Argentine hemorrhagic fever, has been identified using ELISA assays to detect Junin IgG antibodies in infected rodents [58]. More recently, PCR assays have become available for both Lassa and Junin, and a PCR has been developed that predictably amplifies any member of the Arenaviridae family by targeting a highly conserved RNA terminal segment [59].…”

Section: Diagnostic Studiesmentioning

confidence: 99%

Hemorrhagic Fever Viruses

Pigott

2005

Critical Care Clinics

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This article reviews the epidemiology, pathophysiology, and clinical management of patients with suspected or confirmed viral hemorrhagic fever infection. The focus is on clinical management based on case series from naturally occuring outbreaks of viral hemorrhagic fever infection as well as imported cases of viral hemorrhagic fever encountered in industrialized nations. The potential risk of bioterrorism involving these agents is discussed as well as emergency department and critical care management of isolated cases or larger outbreaks. Important aspects of management, including recognition of infected patients, isolation and decontamination procedures, as well as available vaccines and therapies are emphasized.

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“…AHF is a disease of mandatory reporting in Argentina. ELISA is the routine assay for the etiologic confirmation of clinical diagnosis for convalescent patients and for the surveillance of the zoonosis [Riera et al, 1997;Morales et al, 2002]. Nevertheless, the use of the live virus for the production of antigens represents a potential risk and restricts its production to facilities with biosafety levels between 2 and 3 in Argentina, which depends on the strain employed (attenuated or not).…”

Section: Introductionmentioning

confidence: 99%

Argentine hemorrhagic fever diagnostic test based on recombinant Junín virus N protein

Ure

Ghiringhelli

Possee

et al. 2008

Journal of Medical Virology

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Junín arenavirus is the etiologic agent of Argentine hemorrhagic fever. Due to its morbidity and high mortality rate in untreated cases, this endemic disease is of mandatory report in Argentina. Secure and accurate diagnostic methods are needed for the epidemiological surveillance of the disease. Current assays rely on antigens prepared from lysates of virus infected mammalian cells. The bio-safety issue related to the manipulation of large quantities of virus restricts such antigen production to laboratories with the appropriate containment facilities. In this report, we describe the development of an enzyme linked immunosorbent assay for the etiologic confirmation of the disease, based on recombinant antigens expressed in insect cells. Eight different variables of the assay were optimized with the Taguchi approach for experimental design (L18 design, seven three-level factors and one two-level factor). The area under the receiver operating characteristics (ROC) curve was 0.966, showing the high accuracy of the test discriminating positive from negative samples. Taking into account the biosafety benefits, the high yields of antigen in cell culture, and the general performance of the assay, it is expected that it will be a useful alternative to the current ELISA for the detection of antibodies in sera from convalescent patients.

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Evaluation of an enzyme-linked immunosorbent assay for detection of antibodies to Junin virus in rodents

Cited by 15 publications

References 11 publications

Characterization of Monoclonal Antibodies to Junin Virus Nucleocapsid Protein and Application to the Diagnosis of Hemorrhagic Fever Caused by South American Arenaviruses

Characterization of Monoclonal Antibodies to Junin Virus Nucleocapsid Protein and Application to the Diagnosis of Hemorrhagic Fever Caused by South American Arenaviruses

Hemorrhagic Fever Viruses

Argentine hemorrhagic fever diagnostic test based on recombinant Junín virus N protein

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