Evaluation of an in-house loop-mediated isothermal amplification for Mycobacterium tuberculosis detection in a remote reference laboratory, Thailand

Jekloh, Nasron; Keawliam, Pisuttida; Mukem, Daris; Rudeeaneksin, Janisara; Srisungngam, Sopa; Bunchoo, Supranee; Klayut, Wiphat; Bhakdeenaun, Payu; Phetsuksiri, Benjawan

doi:10.1590/s1678-9946202264057

Cited by 3 publications

(2 citation statements)

References 15 publications

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“…Other molecular techniques include ligase chain amplification, for identifying drug resistance mutations, and LAMP, which is faster and less expensive than PCR [ 102 , 103 ].…”

Section: Molecular Methodsmentioning

confidence: 99%

Evolution of Laboratory Diagnosis of Tuberculosis

Zaporojan,

Negrean,

Hodișan

et al. 2024

Clinics and Practice

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Tuberculosis (TB) is an infectious disease of global public health importance caused by the Mycobacterium tuberculosis complex. Despite advances in diagnosis and treatment, this disease has worsened with the emergence of multidrug-resistant strains of tuberculosis. We aim to present and review the history, progress, and future directions in the diagnosis of tuberculosis by evaluating the current methods of laboratory diagnosis of tuberculosis, with a special emphasis on microscopic examination and cultivation on solid and liquid media, as well as an approach to molecular assays. The microscopic method, although widely used, has its limitations, and the use and evaluation of other techniques are essential for a complete and accurate diagnosis. Bacterial cultures, both in solid and liquid media, are essential methods in the diagnosis of TB. Culture on a solid medium provides specificity and accuracy, while culture on a liquid medium brings rapidity and increased sensitivity. Molecular tests such as LPA and Xpert MTB/RIF have been found to offer significant benefits in the rapid and accurate diagnosis of TB, including drug-resistant forms. These tests allow the identification of resistance mutations and provide essential information for choosing the right treatment. We conclude that combined diagnostic methods, using several techniques and approaches, provide the best result in the laboratory diagnosis of TB. Improving the quality and accessibility of tests, as well as the implementation of advanced technologies, is essential to help improve the sensitivity, efficiency, and accuracy of TB diagnosis.

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“…Other molecular techniques include ligase chain amplification, for identifying drug resistance mutations, and LAMP, which is faster and less expensive than PCR [ 102 , 103 ].…”

Section: Molecular Methodsmentioning

confidence: 99%

Evolution of Laboratory Diagnosis of Tuberculosis

Zaporojan,

Negrean,

Hodișan

et al. 2024

Clinics and Practice

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“…One of its notable advantages is its rapid processing and LAMP ability to replicate and amplify huge amounts of DNA. Furthermore, the PURE-LAMP stands out for its cost-effectiveness, as it doesn’t require a thermocycler or expensive equipment (Jekloh et al 2022 ; Notomi et al 2000 ).…”

Section: Assays and Methodsmentioning

confidence: 99%

New insight in molecular detection of Mycobacterium tuberculosis

Mousavi-Sagharchi,

Afrazeh,

Seyyedian-Nikjeh

et al. 2024

AMB Expr

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Mycobacterium tuberculosis, the causative agent of tuberculosis, is a pathogenic bacterium that has claimed millions of lives since the Middle Ages. According to the World Health Organization’s report, tuberculosis ranks among the ten deadliest diseases worldwide. The presence of an extensive array of genes and diverse proteins within the cellular structure of this bacterium has provided us with a potent tool for diagnosis. While the culture method remains the gold standard for tuberculosis diagnosis, it is possible that molecular diagnostic methods, emphasis on the identification of mutation genes (e.g., rpoB and gyrA) and single nucleotide polymorphisms, could offer a safe and reliable alternative. Over the past few decades, as our understanding of molecular genetics has expanded, methods have been developed based on gene expansion and detection. These methods typically commence with DNA amplification through nucleic acid targeted techniques such as polymerase chain reaction. Various molecular compounds and diverse approaches have been employed in molecular assays. In this review, we endeavor to provide an overview of molecular assays for the diagnosis of tuberculosis with their properties (utilization, challenges, and functions). The ultimate goal is to explore the potential of replacing traditional bacterial methods with these advanced molecular diagnostic techniques.

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