Evaluation of an in‐house real‐time polymerase chain reaction method to identify group B streptococcus colonization in pregnancy

Yeung, Sik-Wing; Cheung, Pik-Tsz; Chau, Sze-Lok; Ip, Margaret; Lao, Terence T.; Leung, Thomas; Tam, Wing Hung

doi:10.1111/jog.12724

Cited by 7 publications

(3 citation statements)

References 27 publications

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“…The qPCR reaction showed a specificity of 73.6% when compared to the culture. This result is consistent with that reported by Yeung et al 38 (73.1%), who targeted the cpsG gene locus of the SGB capsular polysaccharide. Both PCR assays presented an NPV of 100%.…”

Section: Discussionsupporting

confidence: 93%

Assessment of conventional PCR and real-time PCR compared to the gold standard method for screening Streptococcus agalactiae in pregnant women

Ferreira

de‐Paris

Paiva

et al. 2018

The Brazilian Journal of Infectious Diseases

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Section: Discussionsupporting

confidence: 93%

Assessment of conventional PCR and real-time PCR compared to the gold standard method for screening Streptococcus agalactiae in pregnant women

Ferreira

de‐Paris

Paiva

et al. 2018

The Brazilian Journal of Infectious Diseases

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“…The negative control should not have an amplification curve for the GBS target, while the positive control should be positive for the two targets tested. To ensure high sensitivity in the PCR, the cutoff point was set at a threshold cycle value of C t = 40 [10, 11].…”

Section: Methodsmentioning

confidence: 99%

Group B Streptococcus detection in pregnant women: comparison of qPCR assay, culture, and the Xpert GBS rapid test

Vieira

Perez

Machado

et al. 2019

BMC Pregnancy Childbirth

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BackgroundGroup B Streptococcus (GBS) is one of the most important causative agents of neonatal sepsis. As administration of prophylactic antibiotics during labor can prevent GBS infection, routine screening for this bacterium in prenatal care before the onset of labor is recommended. However, many women present in labor without having undergone such testing during antenatal care, and the turnaround time of detection methods is insufficient for results to be obtained before delivery.MethodsVaginal and anorectal specimens were collected from 270 pregnant women. Each sample was tested by Xpert GBS, qPCR, and culture for GBS detection.ResultsThe overall prevalence of maternal GBS colonization was 30.7% according to Xpert GBS, 51.1% according to qPCR, and 14.3% according to cultures. Considering the qPCR method as the reference, the Xpert GBS had a sensitivity of 53% and specificity of 93%. Positive Xpert GBS results were correlated to marital status (married or cohabitating) and with prematurity as a cause of neonatal hospitalization. Positive cultures were related with ischemic–hypoxic encephalopathy requiring therapeutic hypothermia.ConclusionsCombined enrichment/qPCR and the Xpert GBS rapid test found a high prevalence of GBS colonization. The Xpert GBS technique gives faster results and could be useful for evaluating mothers who present without antenatal GBS screening results and are at risk of preterm labor, thus allowing institution of prophylactic antibiotic therapy.

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“…PCR methods have reported to have higher sensitivity and detection rates than conventional screening culture, which is viewed as the gold standard method for GBS detection . Previous studies were reported sensitivities for culture were 42.3–68.4%, whereas sensitivity of PCR was 99.6% .…”

Section: Discussionmentioning

confidence: 99%

Detection and genomic analysis of genital group B streptococcus in pregnant Korean women

Lee

Kim

Park

et al. 2018

J of Obstet and Gynaecol

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Evaluation of an in‐house real‐time polymerase chain reaction method to identify group B streptococcus colonization in pregnancy

Abstract: The in-house qPCR test has high sensitivity in detecting GBS colonization. The high negative predictive value helps to avoid unnecessary use of antibiotics in uncolonized women.

Cited by 7 publications

References 27 publications

Assessment of conventional PCR and real-time PCR compared to the gold standard method for screening Streptococcus agalactiae in pregnant women

Assessment of conventional PCR and real-time PCR compared to the gold standard method for screening Streptococcus agalactiae in pregnant women

Group B Streptococcus detection in pregnant women: comparison of qPCR assay, culture, and the Xpert GBS rapid test

Detection and genomic analysis of genital group B streptococcus in pregnant Korean women

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