Evaluation of an octyl group-modified Alaska pollock gelatin-based surgical sealant for prevention of postoperative adhesion

Mizuta, Ryo; Mizuno, Yosuke; Chen, Xi; Kurihara, Yukari; Taguchi, Tetsushi

doi:10.1016/j.actbio.2020.12.025

Cited by 29 publications

(12 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cell numbers were calculated using a standard curve. 13. Biocompatibility and biodegradability test preprint (which was not certified by peer review) is the author/funder.…”

Section: Adhesion and Anti-adhesion Testmentioning

confidence: 99%

“…In contrast, liquid-type tissue adhesives can be delivered using a double-syringe system and cover the tissues without depending on the geometry of the tissue surfaces. [10] Most tissue adhesives are composed of two types of polymer solutions and form gels via chemical reactions such as the thiol-acrylate reaction [11] , amine-aldehyde reaction [12] , and amine-activated ester reaction [13] . Although chemical crosslinking can improve retention time of barriers on the tissues, chemically-crosslinked gels generally represent poor mechanical properties to follow the movement of tissues and non-specific chemical reactions may cause inflammatory responses.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Hotmelt tissue adhesive with supramolecularly-controlled sol-gel transition for preventing postoperative abdominal adhesion

et al. 2022

Self Cite

View full text Add to dashboard Cite

“…Cell numbers were calculated using a standard curve. 13. Biocompatibility and biodegradability test preprint (which was not certified by peer review) is the author/funder.…”

Section: Adhesion and Anti-adhesion Testmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hotmelt tissue adhesive with supramolecularly-controlled sol-gel transition for preventing postoperative abdominal adhesion

et al. 2022

Self Cite

View full text Add to dashboard Cite

“…Hydrogel-derived adhesion barrier materials not including an immunomodulatory agent harbor clinical potential on their own due to hydrophilic surface properties encouraging decreased contact time between regions of mesothelial tissue injury. Recent investigations have focused on physically − and chemically − crosslinked hydrogels in various animal models. However, native hydrogels present brittle mechanical properties, are either deposited via an intricate syringe-based technique or prefabricated for deposition, or employ toxic initiators or curing steps for gelation. , Such a combination greatly limits their clinical utility in adhesion prevention due to the dynamic shifting of organs and tissue surfaces in vivo and either complex or non-biocompatible methods of material generation.…”

Section: Introductionmentioning

confidence: 99%

Controlled Release of a Therapeutic Peptide in Sprayable Surgical Sealant for Prevention of Postoperative Abdominal Adhesions

Erdi

Saruwatari

Rozyyev

et al. 2023

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Formation of asymmetric, rigid scar tissue known as surgical adhesions is caused by traumatic disruption of mesothelial-lined surfaces in surgery. A widely adopted prophylactic barrier material (Seprafilm) for the treatment of intra-abdominal adhesions is applied operatively as a pre-dried hydrogel sheet but has reduced translational efficacy due its brittle mechanical properties. Topically administered peritoneal dialysate (Icodextrin) and anti-inflammatory drugs have failed to prevent adhesions due to an uncontrolled release profile. Hence, inclusion of a targeted therapeutic into a solid barrier host matrix with improved mechanical properties could provide dual utility in adhesion prevention and as a surgical sealant. Spray deposition of poly(lactide-co-caprolactone) (PLCL) polymer fibers through solution blow spinning has yielded a tissue-adherent barrier material with previously reported adhesion prevention efficacy due to a surface erosion mechanism that inhibits deposition of inflamed tissue. However, such an approach uniquely presents an avenue for controlled therapeutic release through mechanisms of diffusion and degradation. Such a rate is kinetically tuned via facile blending of "high" molecular weight (HMW) and "low" molecular weight (LMW) PLCL with slow and fast biodegradation rates, respectively. Here, we explore viscoelastic blends of HMW PLCL (70% w/v) and LMW PLCL (30% w/v) as a host matrix for anti-inflammatory drug delivery. In this work, COG133, an apolipoprotein E (ApoE) mimetic peptide with potent anti-inflammatory properties was selected and tested. In vitro studies with PLCL blends presented low (∼30%) and high (∼80%) percent release profiles over a 14-day period based on the nominal molecular weight of the HMW PLCL component. Two independent mouse models of cecal ligation and cecal anastomosis significantly reduced adhesion severity versus Seprafilm, COG133 liquid suspension, and no treatment control. The synergy of physical and chemical methods in a barrier material with proven preclinical studies highlights the value of COG133-loaded PLCL fiber mats in effectively dampening the formation of severe abdominal adhesions.

show abstract

“…In our previous study, Alaska pollock-derived gelatin (ApGltn) was used to develop tissue sealants by cross-linking with a PEG cross-linker, which was already in practical use. − Unlike the high-transition temperature of porcine gelatin (31.2 °C), ApGltn has a lower denaturation temperature (21.2 °C) and does not need to be preheated before using as a sealant. , Besides, we also developed the anti-inflammatory sealant for sealing and treating wounds with inflammation . To incorporate anti-inflammatory properties in a sealant, ω-3 polyunsaturated fatty acids (PUFAs) that exhibit antioxidant and anti-inflammatory properties were employed to modify ApGltn. − The modification of alkyl group with longer chain length was expected to decrease the water solubility of modified ApGltn .…”

Section: Introductionmentioning

confidence: 99%

Fish Gelatin-Based Absorbable Dural Sealant with Anti-inflammatory Properties

Mizuno

Taguchi

2021

ACS Biomater. Sci. Eng.

Self Cite

View full text Add to dashboard Cite

Cerebrospinal fluid (CSF) leakage from the dura mater during craniotomy is a common complication, which is associated with infection, meningitis, pneumocephalus, and delayed wound healing. In the present study, we developed an absorbable fish gelatin-based anti-inflammatory sealant for dura mater sealing to prevent CSF leakage. Gelatin derived from Alaska pollock (ApGltn) was modified with α-linolenic acid (ALA), an omega-3 fatty acid that exhibits anti-inflammatory properties, and cross-linked with a poly(ethylene glycol)-based cross-linker to develop ALA-ApGltn sealant (ALA-Seal). ALA-Seal demonstrated a higher storage modulus and tangent delta (tan δ) compared with those of the original ApGltn sealant (Org-Seal). The swelling ratio of ALA-Seal was markedly lower than that of DuraSeal, a commercially available dural sealant. Ex vivo burst strength measurements using porcine dura mater indicated that there was no significant difference between DuraSeal and ALA-Seal, despite ALA-Seal having an order of magnitude lower storage modulus. The anti-inflammatory properties of ALA-Seal, evaluated using brain microglial cells, were considerably higher than those of DuraSeal and Org-Seal, with a minimal adverse effect on cell viability. Therefore, compared to DuraSeal, ALA-Seal is a potential dural sealant with a lower swelling ratio, similar burst strength, and higher anti-inflammatory properties, which may prevent CSF leakage.

show abstract

Evaluation of an octyl group-modified Alaska pollock gelatin-based surgical sealant for prevention of postoperative adhesion

Cited by 29 publications

References 51 publications

Hotmelt tissue adhesive with supramolecularly-controlled sol-gel transition for preventing postoperative abdominal adhesion

Hotmelt tissue adhesive with supramolecularly-controlled sol-gel transition for preventing postoperative abdominal adhesion

Controlled Release of a Therapeutic Peptide in Sprayable Surgical Sealant for Prevention of Postoperative Abdominal Adhesions

Fish Gelatin-Based Absorbable Dural Sealant with Anti-inflammatory Properties

Contact Info

Product

Resources

About