Evaluation of Anionic Components of Lead on Biotoxicity and Bioaccumulation Ability in Respect of Probiotic Stamps

Abstract

Lead is one of the most dangerous xenobiotic elements. It is actively accumulated not only in the environment, the distribution of lead in the water-soil-plant-animal-man chain directly depends on the initial content of elements in the geochemical province and is directly related to the health of the population.[1]. This paper presents the results of a study of the biotoxicity of lead cations in the structure of salts with different anionic components in relation to bacteria of the genus Bacillus, which are part of the probiotic preparations. These microorganisms are representatives of the soil microflora and are transitory to the organism of animals and humans. When conducting research, methods such as the agar wells were used, which not only visually but also qualitatively evaluate the effect of metal cations on the growth of the microorganisms studied, the colorimetric method was used to assess the effect of the element under study on the growth of the microorganism population, and the atomic absorption method made it possible to study accumulating characteristics of the studied bacteria. As a result of research, it has been established that lead acetate has a more pronounced toxic effect on the microorganisms under study. It should be noted that lead, which is present in the medium in high concentrations, does not have an inhibitory effect on bacterial strains; we associate this with the detoxification mechanisms of bacteria. The accumulating ability of microorganisms of the Bacillus genus of lead cations from nutrient substrates 24 hours after their cultivation in the presence of this element has high sorption characteristics with a percentage of its accumulation of more than 50%, the most active lead accumulates B. subtilis 534 with 66.3%, and the minimum values were recorded in B. amyloliquefaciens 10642 and amounted to 53.2%.

Preparation of Honeycomb-Structured Activated Carbon–Zeolite Composites from Modified Fly Ash and the Adsorptive Removal of Pb(II)

“…Several methods are available to mitigate heavy metal contamination in aqueous solutions, including electrolysis, oxidation–reduction, and membrane separation. − However, many of these methods have high operating costs and low separation efficiencies in wastewater with a low lead content. Therefore, adsorbent separation processes have attracted the attention of researchers because of their relatively simple operational processes, low potential for byproduct formation, low cost, and easy regeneration procedures. , Many adsorbents have been synthesized from activated carbon, , biomaterials, , and zeolites. − Among the many adsorbents, zeolite has good adsorption properties due to its high specific surface area.…”

Biotoxicity of heavy metal salts to Bacillus subtilis and their sorption properties

“…For many microorganisms, the effectiveness of biological detoxification of metals through targeted microbial activity depends on factors such as the age of culture, the cell shape, pH, contact time, and initial concentrations of metals in the solution [6]. In this regard, several studies with actively growing cells showed that some microorganisms could effectively remove heavy metal ions from the nutrient environment, especially when the concentration of metals was relatively low [7].…”

Fatty acid composition of broiler chicken meat after the combined use of antibiotic and oak bark extract

“…Recently, works discussing the problems and new possibilities of using synthetic preparations and natural substances in the diet of farm animals have appeared. A broad class of antibiotics, extracts, and preparations obtained from oak bark [9][10][11] can be considered as an example of combined use. The aim was to study the combined use of chlortetracycline and Quercus cortex extract.…”