Evaluation of Anthocyanin Profiles in Various Blackcurrant Cultivars over a Three-Year Period Using a Fast HPLC-DAD Method

Abstract

Anthocyanins are the most important polyphenolic substances contained in blackcurrant fruits. They are responsible for the various health benefits caused, in particular, by their high antioxidant activity. Anthocyanins derived from anthocyanidins cyanidin and delphinidin are typical for blackcurrant fruits, especially their rutinoside and glucoside forms. These four anthocyanins usually represent about 97–98% of total anthocyanins in blackcurrant fruits. In this study, we developed and validated a new HPLC-DAD method for rapid anthocyanin separation and determination in fifteen perspective blackcurrant cultivars (‘Ruben’, ‘Ben Lomond’, ‘Ben Conan’, ‘Ceres’, ‘Moravia’, ‘Ometa’, ‘Lota’, ‘Fokus’, ‘Tenah’, ‘Sejanec’, ‘Consort’, ‘Triton’, ‘Ben Hope’, ‘Ben Gairn’, and one gooseberry hybrid ‘Josta’). Eight of them were monitored throughout the three-year experiment. The most represented anthocyanins in all monitored blackcurrant cultivars were delphinidin-3-rutinoside (36.7–63.6%), cyanidin-3-rutinoside (26.4–40.6%), delphinidin-3-glucoside (6.1–17.9%), and cyanidin-3-glucoside (1.3–9.9%). The individual anthocyanin proportion (%) in each cultivar was specific, and a similar profile was verified in a three-year period for eight available cultivars. Total anthocyanin content expressed as a sum of four major anthocyanins present in blackcurrants was compared with values expressed as the equivalent of cyanidin-3-glucoside, as many authors do. We revealed an underestimation of about 20% with the latter method. Cultivars with the highest average total anthocyanin content were ‘Ben Gairn’ (294.38 mg/100 g), ‘Ceres’ (281.31 mg/100 g), and ‘Ometa’ (269.09 mg/100 g).

Antioxidant Extract from Cleistocalyx nervosum var. paniala Pulp Ameliorates Acetaminophen-Induced Acute Hepatotoxicity in Rats

“…For these reasons, the value observed using a pH-differential method would be lower than that which had been obtained using HPLC analysis. Accordingly, this determination is in line with what has been reported in various published articles [25][26][27]. Several studies have suggested that anthocyanins could effectively ameliorate the liver injuries caused by APAP administration in rats and mice through the inhibition of lipid peroxidation, improved GSH depletion, and by inducing antioxidant enzymes.…”

Dietary Intervention with Blackcurrant Pomace Protects Rats from Testicular Oxidative Stress Induced by Exposition to Biodiesel Exhaust

“…Dietary intervention with BC significantly reduced the increased concentration of TBARS, 7-KCh, and LOOHs in the testes of rats exposed to DEE. These results are in line with previous studies, showing the protective effects of anthocyanins from blackcurrants against oxidative stress and other redox-related responses [ 83 , 84 ]. Our results showed the high concentrations of anthocyanins in blackcurrant pomace, mainly cyanidin-3-glucoside, delphinidin-3-O-glucoside, cyanidin-3-O-rutinoside, and delphinidin-3-O-rutinoside ( Supplementary Materials ), which possess the ROS-scavenging activity against hydroxyl radicals and superoxide, and metal-chelating abilities in vitro and in vivo [ 22 , 85 , 86 ].…”

Role of phenylalanine/tyrosine ammonia lyase and anthocyanidin synthase enzymes for anthocyanin biosynthesis in developing Solanum melongena L. genotypes

“…Furthermore, anthocyanins were extracted and characterized using HPLC‐mass spectrometry (HPLC‐MS) from different genotypes. HPLC‐MS is a sensitive method for quantifying the anthocyanins in samples (Šimerdová et al, 2021). Data generated from the present study allowed us to compare the enzyme activities, anthocyanin contents, and correlation between these traits.…”