Evaluation of Anti-inflammatory and Antioxidant Activity of Premna integrifolia Root

Abstract

Roots of Premna integrifolia Linn. Mant. (Verbanacea) are important rasayana (Adaptogenic) drugs and are considered to be useful in the treatment of variety of ailments. The present study was aimed at evaluating the for its anti-inflammatory and antioxidant activities. Pretreatment with a single dose of methanolic extract of P. integrifolia (PIM) (300 mg/kg b.w.) produced significant inhibition on carrageenan-induced rat hind paw edema, histamine induced wheal formation, and acetic acid-induced mouse vascular permeation. In a 7-day study, daily administration of PIM suppressed formalin induced paw edema and cotton pellet-induced rat granuloma formation. The extract also showed significant inhibition of cyclo-oxygenase (COX-I) activity on rat uterus and plasma membrane stabilization. Apart from this, the antioxidant activity (in vitro) of the extracts was evaluated using the anti radical, superoxide scavenging, erythrocyte membrane stability, anti lipid peroxidation, hydroxyl radical scavenging, nitric oxide scavenging and reducing power (ferric thiocynate method and β-carotene bleaching test) assays. PIM showed significant anti-oxidant activity. In addition, PIM (300mg/kg b.w.) produced no observable sub acute toxicity in mice with in 15 days. The results scientifically demonstrated the antiinflammatory activity of P. integrifolia roots in various experimental models probably through their antihistaminic, antikinin, COX-inhibitory and antioxidant action. This therefore justifies the folkloric use of the plant.

Effect of aqueous enriched fraction of Premna integrifolia root against cafeteria diet induced obesity in Swiss albino mice

“…[14] Moreover, alkaloids, carbohydrates, amino acids, steroids, flavonoids, glycosides, tannins and phenolic compounds were found in preliminary phytochemical screening of the aqueous enriched fraction of P. integrifolia root (AEFPIR, aqueous extract). [15] P. integrifolia has been reported for its potential actions such as antidiabetic, hypoglycemic and antihyperglycaemic, [16][17][18] anti-inflammatory, [7,19,20] immunomodulatory, [21] cardiac stimulant and cardioprotective, [22,23] analgesic and antibacterial, [24] antimicrobial, [25][26][27] anti-arthritic, [19,28] hepatoprotective, [29][30][31] in-vitro cytotoxic and tumor suppression, [31,32] hypolipidemic or anti-hyperlipidemic and anti-obesity, [3,[33][34][35][36] neuroprotective, [37] antiparasitic, [38] antioxidant, [14,20,29,30,32,39,40] anticoagulation, [41] antitumor, [42] gastroprotective and antiulcer [43] and longevity-promoting. [44] The herbal formulations containing P. integrifolia were also evaluated for their memory enhancing, [45] hypolipidemic, [46] and in-vitro bioactivity effects.…”

Ocimum basilicum (L.) and Premna integrifolia (L.) modulate stress response and lifespan in Caenorhabditis elegans

“…Previous studies suggest that plant extract can effectively modulate stress level prolonging lifespan significantly (Peng et al, 2012;Guha et al, 2013;Vayndorf et al, 2013). Most of the knowledge about beneficial effects of O. basilicum and P. integrifolia has been attributed to in vitro studies and less is known about their in vivo effect (Gülç in et al, 2007;Gokani et al, 2011). Therefore, we evaluated and compared the effect of O. basilicum and P. integrifolia on stress level and lifespan in C. elegans.…”

Premnine HCl Estimation in Selected Formulations of ‘Dashmul’ and in Chloroform Extract of Premna integrifolia L. by a Selective, Validated and Developed HPTLC Fingerprint Method

“…Literature survey shows three novel diterpenoids isolated from root bark and simultaneous quantification of it by HPTLC method [4,5] a verbascoside iridoid glycosides from leaves [6], two alkaloid premnine and ganiarine isolated from root [7], volatile constituents isolated from flower buds [8], flavonoids Luteolin 7-O-methyl ether and Apigenin 5,7-O-dimethyl ether isolated from leaves [9], identification of volatile constituents of leaves and roots analysed by GC-MS [10], stem bark and wood alcoholic extracts shows cardiotonic effects and β adrengic effects [11] and also antioxidant effects [12], antimicrobial activity of root extracts [13], wood extract for antiarthritic activity [14], methanolic extract of root evaluated for immunomodulatory effects [15].…”