Evaluation of anti-leishmanial and antibacterial activity of &lt;i&gt;Waldheimia tomentosa&lt;/i&gt; (Asteraceae), and chemical profiling of the most bioactive fraction

Bhatnagar, M; Avasthi, Anupama Sharma; Singh, Sarman; Ghosal, Shibnath

doi:10.4314/tjpr.v16i9.18

Cited by 6 publications

(5 citation statements)

References 18 publications

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“…GC-MS analysis GC-MS analysis was done using the most active fraction (n-hexane fraction) following previous procedures. 14 The equipment used for the analysis was GCMS-Q2010 Ultra, Shimadzu gas chromatograph.…”

Section: Packed Cell Volume (Pcv) Measurementmentioning

confidence: 99%

In vivo Antimalarial and GC-MS Studies of Pennisetum purpureum Leaf Extract and Fractions

2022

TJNPR

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Pennisetum purpureum (elephant grass) is a plant used in ethnomedicine for malaria treatment. The present study was aimed at investigating the antimalarial potential of P. purpureum leaf and to identify its phytoconstituents. The leaves were extracted with methanol to afford the crude extract which was successively partitioned between water and n-hexane, dichloromethane, ethyl acetate and n-butanol to afford the fractions. The acute toxicity study was done in mice. Mice were infected with Plasmodium berghei and then treated (p.o.) with the crude extract in the curative and suppressive antimalarial models at three doses (100, 200 and 400 mg/kg). Another set of infected mice was also treated orally with 200 mg/kg of each of the fractions in a suppressive model. The reference drug used for both models was artemeter/lumefantrine (7 mg/kg A/L). The most active fraction, the n-hexane fraction, was subjected to further analysis by GC-MS. The crude extract lethal dose (LD50) was established as 1702.94 mg/kg. The crude extract showed a plasmodial inhibitory activity in the range of 46.20 to 59.90% in the curative model. Both the extract and fractions displayed chemo-suppressive activity (p<0.05) in the range of 66.90 to 96.50%. The A/L produced (p<0.05) 69.00% inhibitory and 95.20% chemosuppressive activities. The results of GC-MS showed the presence of 21 compounds. It was concluded that the extract and fractions of P. purpureum displayed strong antimalarial activity in both models which provides justification for the use of the plant in traditional medicine for malarial treatment.

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Section: Packed Cell Volume (Pcv) Measurementmentioning

confidence: 99%

In vivo Antimalarial and GC-MS Studies of Pennisetum purpureum Leaf Extract and Fractions

2022

TJNPR

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“…The GC-MS analysis of one of the n-hexane subfractions (ACH3) was done as previously described [16] using a gas chromatograph equipment that was coupled to a mass selective detector (GCMS-QP2010SE Shimadzu, Japan). The n-hexane fraction was chosen for GC-MS analysis as it was the most volatile fraction (thus most suitable for GC-MS analysis).…”

Section: Gc-ms Analysismentioning

confidence: 99%

<I>In vivo</I> antimalarial effect of <I>Ananas comosus</I> (L) Merr (Bromeliaceae) fruit peel, and gas chromatography-mass spectroscopy profiling: A possible role for polyunsaturated fatty acid

Uzor¹,

Ishiwu²,

Nwodo³

2020

Trop. J. Pharm Res

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Purpose: To evaluate the antimalarial effect of Ananas comosus extract and fractions and also to identify the likely bioactive compounds.Method: The fruit peel of the plant was extracted with methanol, and the extract successively fractionated with n-hexane, dichloromethane, ethyl acetate, methanol and water. The n-hexane fraction was further subjected to vacuum liquid chromatography to afford four sub-fractions, one of which was also analyzed using gas chromatography-mass spectroscopy (GC-MS). Plasmodium berghei-infected mice were treated orally with three doses (100, 200 and 400 mg/kg) of the plant extract and a single dose (200 mg/kg) of each of the fractions and sub-fractions in a curative antimalarial model using artemisinin combination therapy (ACT) as the reference drug.Results: The extract exhibited significant (p < 0.001) non-dose dependent parasitemia inhibitory activity in the range of 44.84 to 76.09 %. All fractions displayed inhibitory effect (p < 0.001) in the range of 46.44 to 87.58 % with the dichloromethane fraction displaying the highest effect (87.58 %). The subfractions exhibited significant inhibitory effect (p < 0.001) in the range of 84.14 to 92.54 %. The ACT produced significant (p < 0.001) inhibitory effect of 83.92 %. GC-MS analysis revealed the presence of 17 bioactive compounds, the most abundant of which were linoleic acid and palmitic acid.Conclusion: A. comosus displays strong antimalarial activity which supports the folkloric use of the plant for malarial treatment. A polyunsaturated fatty acid (linoleic acid) was the most abundant phytoconstituent identified. Keywords: Ananas comosus, Antimalarial, Malaria, Pineapple, Plasmodium beighei

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“…Examples of these studies are: Calendula officinalis L. against L. major promastigotes and amastigotes (Nikmehr et al, 2014); Echinacea purpurea (L.) Moench against L. donovani promastigotes (Canlas et al, 2010) and L. major in in vivo infection (Sarkari et al, 2017); Echinops kebericho Mesfin toward L. aethiopica and L. donovani promastigotes and amastigotes (Tariku et al, 2011); Gochnatia pulchra Cabrera against L. amazonensis (Lucarini et al, 2012, 2016); Waldheimia tomentosa (Decne.) Regel against L. major promastigotes and amastigotes (Bhatnagar et al, 2017); and Pulicaria gnaphalodes (Vent.) Boiss.…”

Section: Other Compounds From Asteraceae Plants With Activity Againstmentioning

confidence: 99%

Asteraceae Plants as Sources of Compounds Against Leishmaniasis and Chagas Disease

et al. 2019

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Leishmaniasis and Chagas disease cause great impact on social and economic aspects of people living in developing countries. The treatments for these diseases are based on the same regimen for over 40 years, thus, there is an urgent need for the development of new drugs. In this scenario, Asteraceae plants (a family widely used in folk medicine worldwide) are emerging as an interesting source for new trypanocidal and leishmanicidal compounds. Herein, we provide a non-exhaustive review about the activity of plant-derived products from Asteraceae with inhibitory action toward Leishmania spp. and T. cruzi . Special attention was given to those studies aiming the isolation (or identification) of the bioactive compounds. Ferulic acid, rosmarinic acid, and ursolic acid ( Baccharis uncinella DC.) were efficient to treat experimental leishmaniasis; while deoxymikanolide ( Mikania micrantha ) and (+)-15-hydroxy-labd-7-en-17-al ( Aristeguietia glutinosa Lam.) showed in vivo anti- T. cruzi action. It is also important to highlight that several plant-derived products (compounds, essential oils) from Artemisia plants have shown high inhibitory potential against Leishmania spp., such as artemisinin and its derivatives. In summary, these compounds may help the development of new effective agents against these neglected diseases.

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Evaluation of anti-leishmanial and antibacterial activity of <i>Waldheimia tomentosa</i> (Asteraceae), and chemical profiling of the most bioactive fraction

Cited by 6 publications

References 18 publications

In vivo Antimalarial and GC-MS Studies of Pennisetum purpureum Leaf Extract and Fractions

In vivo Antimalarial and GC-MS Studies of Pennisetum purpureum Leaf Extract and Fractions

<I>In vivo</I> antimalarial effect of <I>Ananas comosus</I> (L) Merr (Bromeliaceae) fruit peel, and gas chromatography-mass spectroscopy profiling: A possible role for polyunsaturated fatty acid

Asteraceae Plants as Sources of Compounds Against Leishmaniasis and Chagas Disease

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