Evaluation of anti-mycobacterial activity of garlic (Allium sativum) against clinical isolates of non-MDR and MDR Mycobacterium tuberculosis

Hannan, Abdul; Ikramullah, M; Qayum, Arem; Shah, K U; Arshad, Usama; Hussain, Syed M.

doi:10.1055/s-0029-1234977

Cited by 7 publications

(8 citation statements)

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“…It is very hurtful to a patient’s health due to high levels of drug toxicity and its adverse effects [6-8]. The emergence of MDR TB and extensively-drug resistant (XDR) TB to the medicines now in use makes urgent search for new anti-TB agents worldwide [9,10].…”

Section: Introductionmentioning

confidence: 99%

In vitro Anti-mycobacterial activity of selected medicinal plants against Mycobacterium tuberculosis and Mycobacterium bovis Strains

Gemechu

Giday

Worku

et al. 2013

BMC Complement Altern Med

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BackgroundTuberculosis (TB) is a global burden with one –third of the world’s population infected with the pathogen Mycobacterium tuberculosis complex and annually 1.4 million deaths occur due to the disease. This high incidence of infection and the increased rate of multi-drug resistant and extensively-drug resistant strains of the organism further complicated the problem of TB control and have called for an urgent need to develop new anti-TB drugs from plants. In this study, the in vitro activity of root of Calpurnia aurea, seeds of Ocimum basilicum, leaves of Artemisia abyssinica, Croton macrostachyus, and Eucalyptus camaldulensis were evaluated against M. tuberculosis and M. bovis strains.MethodsFive Ethiopian medicinal plants, root of Calpurnia aurea, seeds of Ocimum basilicum, leaves of Artemisia abyssinica, Croton macrostachyus, and Eucalyptus camaldulensis used locally for the management of TB. They were investigated for in vitro antimycobacterial activity against M. tuberculosis and M. bovis strains. 80% methanolic extracts of the plant materials were obtained by maceration. The antimycobacterial activity was determined using 96 wells of microplate with the help of visual Resazurin Microtiter Assay.ResultsThe crude 80% methanolic extracts of the root of C. aurea, seeds of O. basilicum, and leaves of A. abyssinica, C. macrostachyus, and E. camaldulensis had anti-mycobacterial activity with minimum inhibitory concentration (MIC) ranging from 6.25–100 μg/mL. The MIC of 80% methanol extracts in the order mentioned above ranged 25-100 μg/ml and 12.5-75 μg/mL, 25–100 μg/mL and 25–50 μg/mL, 6.25-50 μg/mL and 12.5-50 μg/mL, 12.5-100 μg/mL and 18.25-50 μg/mL and 6.25-50 μg/mL and 12.5-50 μg/mL, respectively for M. tuberculosis and M. bovis strains.ConclusionsThe results support the local use of these plants in the treatment of TB and it is suggested that these plants may have therapeutic value in the treatment of TB. However, further investigations are needed on isolating chemical constituents responsible for eliciting the observed activity in these plants.

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Section: Introductionmentioning

confidence: 99%

In vitro Anti-mycobacterial activity of selected medicinal plants against Mycobacterium tuberculosis and Mycobacterium bovis Strains

Gemechu

Giday

Worku

et al. 2013

BMC Complement Altern Med

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“…Other compounds include alkaloids, terpenes, resin, monoterpenoids, sesquiterpenoids and phenols which show activity against Mycobacterium tuberculosis 51 , 15 , 52 . Allium sativum is a plant that has been reported as an established remedy for the treatment of tuberculosis 53 . It possesses variety of biological properties such as anti-cancer, anti-microbial, antioxidant, immunomodulatory, anti-inflammatory, hypoglycaemic and anti-cardiovascular properties 54 .…”

Section: Discussionmentioning

confidence: 99%

“…It possesses variety of biological properties such as anti-cancer, anti-microbial, antioxidant, immunomodulatory, anti-inflammatory, hypoglycaemic and anti-cardiovascular properties 54 . Several studies conducted on the in vitro activity of Allium sativum against Mycobacterium tuberculosis revealed that this plant possesses anti-tubercular properties 41 , 42 , 53 , 53 . The presence of sulphur compounds such as allicin, ajoene, allylmethyltrisulfide, diallyltrisulfide, diallyldisulphide has been associated with the anti-tubercular activity of this Allium sativum 55 .…”

Section: Discussionmentioning

confidence: 99%

Anti-mycobacterium tuberculosis activity of polyherbal medicines used for the treatment of tuberculosis in Eastern Cape, South Africa

Famewo¹,

Clarke²,

Wiid³

et al. 2017

Afr H. Sci.

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BackgroundThe emergence of drug-resistant strains of Mycobacterium tuberculosis has become a global public health problem. Polyherbal medicines offer great hope for developing alternative drugs for the treatment of tuberculosis.ObjectiveTo evaluate the anti-tubercular activity of polyherbal medicines used for the treatment of tuberculosis.MethodsThe remedies were screened against Mycobacterium tuberculosis H37Rv using Middlebrook 7H9 media and MGIT BACTEC 960 system. They were liquid preparations from King Williams Town site A (KWTa), King Williams Town site B (KWTb), King Williams Town site C (KWTc), Hogsback first site (HBfs), Hogsback second site (HBss), Hogsback third site (HBts), East London (EL), Alice (AL) and Fort Beaufort (FB).ResultsThe susceptibility testing revealed that all the remedies contain anti-tubercular activity with KWTa, KWTb, KWTc, HBfs, HBts, AL and FB exhibiting more activity at a concentration below 25 µl/ml. Furthermore, MIC values exhibited inhibitory activity with the most active remedies from KWTa, HBfs and HBts at 1.562 µg/ml. However, isoniazid showed more inhibitory activity against M. tuberculosis at 0.05 µg/ml when compare to the polyherbal remedies.ConclusionThis study has indicated that these remedies could be potential sources of new anti-mycobacterial agents against M. tuberculosis. However, the activity of these preparations and their active principles still require in vivo study in order to assess their future as new anti-tuberculosis agents.

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“…According to Ayurvedic and Greek systems of medicine garlic is one of the established remedies for tuberculosis 5 .…”

Section: Introductionmentioning

confidence: 99%

“…MIC of garlic extract was ranged from 1 to 3 mg/ml; showing inhibitory effects of garlic against both non-MDR and MDR M. tuberculosis isolates. 5 The study on in vitro anti-tubercular activity of five medicinal plants viz., Acalypha indica, Adhatoda vasica, Allium cepa, Allium sativum and Aloe vera suggested the estimation of minimum inhibitory concentration (MIC) in suitable broth based media as MICs defined in broth are more accurate 8 .…”

Section: Introductionmentioning

confidence: 99%

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2013

IJPSR

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Tuberculosis is one of the oldest killer diseases of mankind. It is an airborne communicable disease caused by transmission of aerosolized droplets of Mycobacterium tuberculosis. The increasing incidence of MDR-and XDR-TB worldwide highlighted the urgent need to search for newer anti-tuberculosis compounds/drugs. The World Health Organization (WHO) estimated that approximately 80% of world population relies mainly on traditional medicines, mostly plant drugs in their health care. The higher plant extracts are promising sources of novel anti-TB leads. Screening and estimation of lyophilised aqueous, aqueous ethanol and ethanolic extracts of four traditional Indian medicinal plants Acalypha indica, Adhatoda vasica, Allium cepa and Allium sativum for anti-tubercular activity to standard strain H37Rv, clinical isolate of Isoniazid mono resistant and poly resistant isolates by the sensitive and reliable Broth Microdilution Method (BMM). Inhibition of M. tuberculosis isolates was observed for two of the four medicinal plants. All the three extracts of Allium sativum were found to be active in broth dilution assays with MIC of 500µg/ml and higher against the tested strains. The ethanolic extract of Allium cepa was found to be active with MIC of 500µg/ml against the tested strains. While the extracts of A. vasica and A. indica did not inhibit the growth of M. tuberculosis even at 500 µg/ml. The extracts active against M. tuberculosis were evaluated for cytotoxicity. The maximal toxic free concentration on Vero cells were at 500μg/ml showing that the extracts were not toxic to Vero cells in the concentrations tested.

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Evaluation of anti-mycobacterial activity of garlic (Allium sativum) against clinical isolates of non-MDR and MDR Mycobacterium tuberculosis

Cited by 7 publications

References 0 publications

In vitro Anti-mycobacterial activity of selected medicinal plants against Mycobacterium tuberculosis and Mycobacterium bovis Strains

In vitro Anti-mycobacterial activity of selected medicinal plants against Mycobacterium tuberculosis and Mycobacterium bovis Strains

Anti-mycobacterium tuberculosis activity of polyherbal medicines used for the treatment of tuberculosis in Eastern Cape, South Africa

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