Evaluation of Antibacterial and Synergistic Effect of Lichen Extracts with Antibiotics Against Fire Blight Pathogen, Erwinia amylovora

Lee, Su-In; Kim, Wonjun; Kam, Da-Ran; Kwak, Youn‐Sig

doi:10.7585/kjps.2021.25.1.55

Cited by 2 publications

(1 citation statement)

References 22 publications

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“…Biopesticides for fire blight control may be permitted in Korea if the novelty and biosafety of their microbial sources are proven. Recently, few studies have been conducted to develop domestic BCAs using antagonistic bacteria (Kim et al, 2022), lichen extracts (Lee et al, 2021), and bacteriophages (Park et al, 2018(Park et al, , 2022. Although these BCAs were developed domestically to ensure originality, they came from a single source.…”

Section: Introductionmentioning

confidence: 99%

Screening of Bacterial Antagonists to Develop an Effective Cocktail against Erwinia amylovora

Choi

Kim

et al. 2022

Res. Plant Dis

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Several types of chemical bactericides have been used to control fire blight. However, their excessive usage leads to environmental deterioration. Therefore, several researchers have analyzed antagonistic microorganisms as promising, effective, and safe biological control agents (BCAs). The primary aim of this study was to screen for potential antagonistic bacteria that suppress Erwinia amylovora. Among the 45 isolates studied, 5 strains showed the largest inhibition zone against E. amylovora. 16S rRNA gene sequencing identified them as Bacillus amyloliquefaciens (KPB 15), B. stratosphericus (KPB 21), B. altitudinis (KPB 25), B. safensis (KPB 31), and B. subtilis (KPB 39). KPB 25 and 31 reduced the lesion size of fire blight by 50% in immature apple fruits, and did not show antagonism against each other. Therefore, KPB 25 and 31 were selected to develop an antagonistic mixture against fire blight. Although the mixture with KPB 25 and 31 showed a slightly increased ability to reduce lesion size on immature fruits, they did not exhibit a synergistic effect in reducing E. amylovora population compared to each strain alone. Nevertheless, we have identified these two strains as useful and novel BCAs against fire blight with additional benefits safety and potential in developing a mixture without loss of their activity, owing to the absence of antagonism against each other.

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