Evaluation of Antibacterial Potential of Leaf and Leaf Derived Callus Extracts of Orthosiphon Aristatus (Blume) Miq.

Abstract

Objective: To evaluate the antibacterial efficacy of chloroform, petroleum ether, ethyl acetate, methanol, ethanol and aqueous extracts of leaf and leaf derived callus of Orthosiphon aristatus against Bacillus cereus, Bacillus subtiltis, Staphylococcus aureus, Streptococcus pyogenes, Enterobater aerogenes, Escherichia coli, Proteus mirabilis, and Klebseilla pneumoniae. Methods: The leaf segments were cut into small pieces of size 1-2 sq. cm and were cultured on Murashige and Skoog solid medium supplemented with different auxins alone and in combination. Antibacterial efficacy was performed by disc diffusion method followed my MIC determination by two fold serial dilution method. Leaf and leaf callus extracts were subjected to the qualitative phytochemical analysis. Results: Maximum callus formation percentage was obtained from the leaf segments cultured on MS medium supplemented with 2, 4-D (2 mg/l). Ethanolic leaf extract showed maximum inhibition activity with 28 mm zone of inhibition against P. mirabilis with MIC value of 0.32 mg/ml. Out of the callus extracts, ethanolic callus extract showed the maximum bio-efficacy against S. aureus with 26 mm zone of inhibition and MIC value of 0.64 mg/ml. Results revealed that both leaf and leaf derived callus are effective against Gram positive and Gram negative test bacteria.Conclusion: The bioefficacy study confirms the strong antibacterial potential of leaf and leaf derived callus of O. aristatus.Keywords: Leaf, Leaf callus, Antibacterial efficacy, Orthosiphon aristatus.

The Medicinal Plants Used in Anjir Pulang Pisau, Central Kalimantan-Indonesia

“…Some of these microorganism are the cause of urinary tract infections. [9][10] The local people used Arcangelisia flava (L.) Merr. to cure liver.…”

Phytochemical content and antioxidant capacity of ethyl acetate extracts from fifteen Orthosiphon aristatus leaves genotypes

“…The leaves of this plant are commonly prepared as herbal tea to treat various ailments, including rheumatism, diabetes, urinary lithiasis, edema, eruptive fever, influenza, hepatitis, jaundice, and hypertension (Hossain and Rahman 2015). Several studies have been conducted on the pharmacological activities of O. aristatus, including its antioxidant properties (Cai et al 2018), inhibition of the α-glucosidase enzyme (Mohamed et al 2015), antidiabetic properties (Lokman et al 2019), antiobesity properties (Seyedan et al 2017), anticancer properties (Movahedi et al 2015), prevention of urinary system infections (Sarshar et al 2017), antiosteoporotic properties (Bokhari et al 2018), and antibacterial properties (Reshi et al 2017).…”