Evaluation of antibiofilm effect of benzalkonium chloride, iodophore and sodium hypochlorite against biofilm of Pseudomonas aeruginosa of dairy origin

Abstract

The present study was undertaken with objectives of; a) to investigate and compare Pseudomonas aeruginosa isolates from two dairies for biofilm formation potential and, b) to compares three common biocides for biofilm eradication efficiencies. Amongst the isolates from commercial dairy, 70 % were strong and/or moderate biofilm former in comparison to 40 % isolates from small scale dairy. All isolates, irrespective of source, exhibited higher susceptibility to biocides in planktonic stage than in biofilm. Antibiofilm efficiencies of three biocides i.e. benzalkonium chloride, sodium hypochlorite and iodophore were determined in terms of their microbial biofilms eradicating concentration (MBEC). Our findings show that the three biocides were ineffective against preformed biofilms at recommended in-use concentrations. Biofilms were the most resistant to benzalkonium chloride and least against iodophore. A trend of decreasing MBECs was observed with extended contact time. The findings of present study warrant for a systematic approach for selecting types and concentrations of biocide for application as antibiofilm agent in food industry.

Response of Formed‐Biofilm of Enterobacter cloacae, Klebsiella oxytoca, and Citrobacter freundii to Chlorite‐Based Disinfectants

“…In fact, the disinfection by SH is greatly dependent on the strains of bacteria. Jahid and Ha () reported reductions of 6.5 and 6.7 log CFU/cm 2 after a 30‐min treatment with SH of Aeromonas hydrophila planktonic cells, whereas similar effects have been observed with 2000 and 4000 mg/L for biofilm cells, and in a recent study, SH was not very effective in killing biofilms (Pagedar & Singh, ). The presence of organic materials can also decrease the bactericidal effect of disinfectants along with a decrease in the ACC (Fukuzaki, ).…”

“…There has been a substantial amount of research in recent years into bactericides effective against Pseudomonas spp. biofilms (Behnke & Camper, 2012;Byun, Kim, Kim, Kim, & Jo, 2007;Malheiro et al, 2016;Pagedar & Singh, 2015). However, little research has been done to inactivate cells in ENT biofilms, and few studies have used disinfectants to treat ENT biofilms.…”

Biofilms formed within the acidic and the neutral biotrickling filters for treating H2S-containing waste gases

“…19 The formation of the biolm has been found to be related with the microbial species, surface properties and other various environmental factors. [20][21][22] However, only limited investigation has been done regarding biolms formed in acidic conditions.…”

Electrochemical Preparation of Synergistic Nanoantimicrobials

“…The latter belongs to the quaternary ammonium compounds, also known as quats , having a broad spectrum of antimicrobial activity and used as disinfectants in hospitals [20]. BAC in particular is an asymmetric alkyl ammonium salt, well-known for its antimicrobial and antibiofilm properties [21].…”