Evaluation of anticoagulant activity of two algal polysaccharides

Abstract

Marine algae are important sources of phycocolloids like agar, carrageenans and alginates used in industrial applications. Algal polysaccharides have emerged as an important class of bioactive products showing interesting properties. The aim of our study was to evaluate the potential uses as anticoagulant drugs of algal sulphate polysaccharides extracted from Ulva fasciata (Chlorophyta) and Agardhiella subulata (Rhodophyta) collected in Ganzirri Lake (Cape Peloro Lagoon, north-eastern Sicily, Italy). Toxicity of algal extracts through trypan blue test and anticoagulant action measured by activated partial thromboplastin time (APTT), prothrombin time (PT) test has been evaluated. Algal extracts showed to prolong the PT and APTT during the coagulation cascade and to avoid the blood coagulation of samples. Furthermore, the algal extracts lack toxic effects towards cellular metabolism and their productions are relatively at low cost. This permits to consider the algae as the biological source of the future.

Characterization and Comparison of the Structural Features, Immune-Modulatory and Anti-Avian Influenza Virus Activities Conferred by Three Algal Sulfated Polysaccharides

“…Sulfated polysaccharides from algae have been acknowledged to bear diversified biological and pharmacological functions [ 22 , 60 , 61 , 62 ]. Moreover, the chemical composition and structural features of polysaccharides were confirmed to function as determinants of their bioactivities [ 17 , 57 ].…”

Chemical characterization of sulfated polysaccharides from Gracilaria gracilis and Ulva lactuca and their radical scavenging, metal chelating, and cholinesterase inhibitory activities

“…Previous studies have shown that algal polysaccharides exhibit antioxidant, [12] , anticancer [13] and anticoagulant activities. [14] However, their neuroprotective effect has not been fully explored.…”

Effect of dietary supplementation of palm fruit extracts on the transcriptomes of growth, antioxidant enzyme and immune-related genes in common carp (Cyprinus carpio ) fingerlings

“…). There is growing trend in the replacement of chemical drugs with herbal medicine and algal extracts, due to their potential health benefits with limited side effects (Peddie, Zou & Secombes ; Najafian & Babji ; Faggio, Morabito, Minicante, Piano, Pagano & Genovese ; Faggio, Pagano, Dottore, Genovese & Morabito ). Therefore, the study about immunostimulants, especially those of plant origin, which have antioxidant and immune property, has remarkably increased in recent years (Galina, Yin, Ardo & Jeney ).…”

“…The use of immunostimulants in the aquaculture industry has been important in reducing antibiotics use and economic losses which occur as a result of disease (Citarasu 2010;Genovese, Faggio, Gugliandolo, Torre, Span o, Morabito & Maugeri 2012;Hoseinifar, Esteban, Cuesta & Sun 2015;Hoseinifar et al 2016). There is growing trend in the replacement of chemical drugs with herbal medicine and algal extracts, due to their potential health benefits with limited side effects (Peddie, Zou & Secombes 2002;Najafian & Babji 2012;Faggio, Morabito, Minicante, Piano, Pagano & Genovese 2015;Faggio, Pagano, Dottore, Genovese & Morabito 2015). Therefore, the study about immunostimulants, especially those of plant origin, which have antioxidant and immune property, has remarkably increased in recent years (Galina, Yin, Ardo & Jeney 2009).…”