Evaluation of anticoagulant and thrombolytic activity of Berberis orthobotrys in animal model

Abstract

<p class="Abstract">Fundamental to this research was the evaluation of anticoagulant and thrombolytic potential of Berberis orthobotrys using clotting time, prothrombin time and activated partial thromboplastin time performed at 2.5, 5, 10% concentration. Aqueous-methanol extract significantly (p<0.05) increased the coagulation parameters both in human blood as well as in rabbit blood and exhibited the more salient effect on prolongation of these coagulation parameters at 10% concentration. Aqueous methanol extract (25, 50 and 100 mg/kg) produced the preferential increase in bleeding time and clotting time nevertheless the effect was more perceptible after 90 min at 100 mg/kg. Moreover, the crude extract presented a substantial increase in coagulation parameters after 7 days of administration in rabbits with more pronounced effects at the highest dose. HPLC profile of aqueous methanol extract revealed the presence of quercetin, p-coumaric acid, gallic acid, vanillic acid, 4-hydroxy-3-methoxy benzoic acid. Thus, the present study provides the pharmacological basis for its medical use in cardiovascular and other thrombotic disorders.</p>

Antihyperglycemic, Neuropharmacological, Cytotoxic, Anticoagulant, and Anti-inflammatory Pharmacological Evaluations of Vernonia elaeagnifolia DC Leaves Secondary Bioactive Metabolites

“…The extract's in-vitro anticoagulant activity is measured by measuring the time it takes a blood sample to clot, also known as prothrombin time. Prolongation of Prothrombin time is used to assess an agent's anticoagulant activity because it indicates the blockage of the extrinsic clotting factor pathway, which includes factors V, VII, and X, all of which are generally activated by vitamin K [45]. In the current study, crude extracts showed a significant (*p<0.05, and ***p<0.001) and concentration-dependent increase in prothrombin time when compared to the control (Fig.…”

“…7). Because Coumarin can inhibit the activation of the abovementioned vitamin K-dependent clotting factors, the anticoagulant effect of the crude extract could be attributed to the presence of Coumarin in plant extract [45], and [46]. Furthermore, the plant extract is said to be rich in flavonoid and alkaloid compounds that exhibit anticoagulant activity by forming a complex with clotting factors to inhibit the conversion of prothrombin to thrombin and, as a result, prevent the conversion of soluble fibrinogen to insoluble fibrin clot [47], and [48].…”

“…The crude extract's antiinflammatory activity in reducing paw edema could be characterized by inhibiting the release of chemical mediators such as histamine, bradykinin, serotonin, and prostaglandin, among others. It has been reported that the plant extract is enriched with alkaloids, flavonoids, tannins, and coumaric acid, all of which have anti-inflammatory properties [3], and [45]. Flavonoids reduce inflammation by blocking molecules such as COX, cytokines, TNF-α, and matrix metalloproteinases [50].…”