Evaluation of antidiabetic activity of polysaccharide isolated from Phellinus linteus in non-obese diabetic mouse

Kim, Hwan Mook; Kang, Jong Soon; Kim, Jee Youn; Park, Song‐Kyu; Kim, Hyungsook; Lee, Young June; Yun, Jieun; Hong, Jin Tae; Kim, Youngsoo; Han, Sang‐Bae

doi:10.1016/j.intimp.2009.09.024

Cited by 67 publications

(35 citation statements)

References 36 publications

(51 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As statistical analyses failed to correlate phenolics and α-glucosidase inhibitory activities, other highly polar phytochemicals may have contributed to these effects. Plant components other than phenolics, such as polysaccharides [57,58] or peptides [59,60] have been shown to possess antidiabetic activities and these may have contributed to the observed inhibition. It is also evident from the literature that some plants contain insulin-like peptides which give the hypoglycemic ability [61].…”

Section: Discussionmentioning

confidence: 99%

Antidiabetic and antioxidant properties of Ficus deltoidea fruit extracts and fractions

Misbah

Aziz

Aminudin

2013

BMC Complement Altern Med

103

View full text Add to dashboard Cite

BackgroundDiabetes is a serious metabolic disorder affecting the metabolism of carbohydrate, protein and fat. A number of studies have shown that diabetes mellitus is associated with oxidative stress, leading to an increased production of reactive oxygen species. Ficus deltoidea is traditionally used in Malaysia for regulating blood sugar, blood pressure and cholesterol levels. The use of F. deltoidea as an alternative medicinal herb is increasingly gaining popularity with the sale of F. deltoidea tea bags and capsules in the local market. The present study was undertaken to investigate the antidiabetic and antioxidant activities of the fruits from different varieties of F. deltoidea, employing in vitro methods.MethodTwo fruit varieties of F. deltoidea (var. angustifolia (SF) and var. kunstleri (BF)) were extracted separately using double-distilled water. The resulting aqueous extracts were partitioned using ethyl acetate to obtain the ethyl acetate and water fractions. The crude aqueous extracts and the corresponding fractions were evaluated for their phenolic, flavonoid, sugar and protein contents. Protein profiling of the extracts and fractions were also carried out by means of SDS-PAGE and SELDI-TOF MS. Antidiabetic activities were assessed based on the ability of the samples to inhibit yeast and mammalian α-glucosidase as well as α-amylase. Antioxidant capacities were examined by measuring the ability of the samples to reduce ferric ions and to scavenge DPPH, superoxide anion, ABTS and nitric oxide radicals.ResultsThe crude extracts and fractions of SF and BF inhibited both yeast and rat intestinal α-glucosidases in a dose-dependent manner, but did not inhibit porcine pancreatic α-amylase. The water fraction of BF showed the highest percentage of α-glucosidase inhibition while having the highest amount of protein (73.33 ± 4.99 μg/mg fraction). All the extracts and fractions exhibited antioxidant activities, with SF crude extract showing the highest antioxidant activity and phenolic content (121.62 ± 4.86 mg/g extract). Fractionation of the crude extracts resulted in loss of antioxidant activities. There was no positive correlation between phenolic and flavonoid content with α-glucosidase inhibitory activities. However, phenolic content correlated well with antioxidant activities of the crude extracts but not with the fractions.ConclusionsThe antioxidant activities of the fruits of F. deltoidea might be asserted by the phenolic content but other polar plant components were possibly involved in the antidiabetic properties. The study of these compounds having both antihyperglycemic and antioxidant activities may provide a new approach in the treatment of diabetes mellitus.

show abstract

Section: Discussionmentioning

confidence: 99%

Antidiabetic and antioxidant properties of Ficus deltoidea fruit extracts and fractions

Misbah

Aziz

Aminudin

2013

BMC Complement Altern Med

103

View full text Add to dashboard Cite

show abstract

“…These effects include reduced tumor growth and frequency of pulmonary metastasis without toxic effects (Han et al 1999). Recent studies have elucidated some of the molecular mechanisms responsible for the induction of apoptosis in tumor cells and clarified components of the apoptotic pathway Guo et al 2007;Zhu et al 2007;Sliva et al 2008;Lu et al 2009;Kim et al 2010). Nevertheless, the inhibition of invasive behaviors and the induction of apoptosis by PL in HepG2 human HCC cells have not been fully addressed.…”

Section: Discussionmentioning

confidence: 99%

Polysaccharides from Phellinus linteus inhibit cell growth and invasion and induce apoptosis in HepG2 human hepatocellular carcinoma cells

et al. 2012

View full text Add to dashboard Cite

It has been demonstrated that the medicinal mushroom Phellinus linteus (PL), which consists mainly of polysaccharides, possesses antitumor and immunomodulatory properties in vivo and in vitro. The mechanism, however, by which PL inhibits growth and invasive behavior of HepG2 cells remains poorly understood. Here we demonstrated that PL inhibited proliferation and colony formation of HepG2 and that the growth inhibition of HepG2 cells was mediated by S-phase cell cycle arrest. PL also markedly inhibited cancer cell adhesion and invasion of the extracellular matrix. Additionally, we demonstrated that PL-induced apoptosis was associated with a reduction in B-cell lymphoma 2 levels and an increase in the release of cytochrome c. These results suggest that PL exerts a direct antitumor effect by initiating apoptosis and cell cycle blockade in HepG2 cells.

show abstract

“…Recent studies on this mushroom have revealed many interesting biological activities (Hsieh and others ). The fruiting bodies of PL have recently been reported to contain various bioactive compounds and exhibit numerous biological activities, including antitumor (Bae and others ), antioxidant (Song and others ), anti‐inflammatory (Kim and others ), antiangiogenic (Lee and others ), antibacterial (Hur and others ), antimutagenesis (Kimura and others ), antidiabetic (Kim and others ), and antihepatotoxin effects (Kim and others ). Several aromatic compounds have also been isolated from the cultured PL mycelia, including sesquiterpene (Huang and others ), phellinstatin (Cho and others ), polyphenol (Jung and others ), hispolon (Chen and others ), hispidin (Jang and others ), and inotilone (Huang and others ).…”

Section: Introductionmentioning

confidence: 99%

Isolation, Purification, and Structural Features of a Polysaccharide from Phellinus linteus and Its Hypoglycemic Effect in Alloxan‐Induced Diabetic Mice

Zhao

Liao

et al. 2014

Journal of Food Science

View full text Add to dashboard Cite

Phellinus linteus is a medicinal mushroom that has been used in Oriental countries for centuries for its antitumor, antioxidant, immunomodulatory, and biological activity on hyperglycemia. A water-soluble crude polysaccharide was extracted using hot water from P. linteus mycelia grown under submerged culture. An orthogonal experiment was used to optimize the extraction conditions of P. linteus mycelia polysaccharides (PLP). The crude polysaccharide was purified using DEAE Sephadex A-50 and Sephadex G-200 chromatography. Fourier transform infrared (FT-IR) spectroscopy and nuclear magnetic resonance ((1) H NMR) spectroscopy were used to investigate the structure of the purified P. linteus polysaccharide (PLP-I), revealing that it was mainly a branched-type glycan with both α- and β-linkages and a pyranoid sugar ring conformation. PLP orally administered at 100 mg/kg body weight/d could significantly reduce the blood glucose level by 35.60% in alloxan-induced diabetic mice. The results of an oral glucose tolerance test (OGTT) revealed that PLP had an effect on glucose disposal after 28 d of treatment. The result revealed that PLP from a submerged culture of P. linteus mycelia possessed potent hypoglycemic properties. The polysaccharide may be useful as a functional food additive and a hypoglycemic agent.

show abstract

Evaluation of antidiabetic activity of polysaccharide isolated from Phellinus linteus in non-obese diabetic mouse

Cited by 67 publications

References 36 publications

Antidiabetic and antioxidant properties of Ficus deltoidea fruit extracts and fractions

Antidiabetic and antioxidant properties of Ficus deltoidea fruit extracts and fractions

Polysaccharides from Phellinus linteus inhibit cell growth and invasion and induce apoptosis in HepG2 human hepatocellular carcinoma cells

Isolation, Purification, and Structural Features of a Polysaccharide from Phellinus linteus and Its Hypoglycemic Effect in Alloxan‐Induced Diabetic Mice

Contact Info

Product

Resources

About