Evaluation of Antidiabetic Activity of a Novel Polyherbal Preparationagainst Streptozotocin Induced Diabetes Rat Model

Abstract

Background: Diabetes Mellitus is a chronic disorder characterised by abnormally elevated glucose levels in the blood. Diabetes is caused by one of two mechanisms: insufficient insulin synthesis (which is produced by the pancreas and reduces blood glucose) or insufficient response of cells to insulin action. The current aim of this research project was to formulate and evaluate the Polyherbal preparation (PHP) of the plants constituted with Cinnamonum zeylanicium (CJ) bark, Eugenia jambolana (EJ) seeds, Vinca rosea (VR) whole plant, Gymnema sylvestre (GS) leaves and determination of the anti-diabetic potential of the formulation in the animal model induced by Streptozotocin.
 Methods: Plant components in the current study used were Cinnamonum zeylanicium (CJ) bark, Eugenia jambolana (EJ) seeds, Vinca rosea (VR) whole plant, Gymnema sylvestre (GS) leaves were collected.Using a hydroalcoholic solvent, physico-chemical parameters and active chemical constituents were evaluated. The active components present in the extracts were identified by Preliminary phytochemical screening. The PHP acute toxicity analysis was conducted in compliance with OECD Guideline 423, with 200 mg/kg and 4000 mg/kg administered orally to rats over 28 days.
 Results: Diabetes was induced by STZ and treated with PHF did not show any alterations in behavior and no mortality was observed up to the 2000 mg/kg dose level during the interventional period. By oral administration of PHP with a dosage of 200 and 400 mg/kg, OGTT resulted in a steady decrease in blood glucose levels of 68.74±4.63 mg/dl and 63.83±1.74 mg/dl at 180min after the trial which proves that PHP possess anti-diabetic activity. By mixing each extract in varying proportions, PHP was developed and evaluated. PHP (200 and 400mg/kg) antidiabetic activity wasdetermined for streptozotocin (STZ)-induced diabetes in rats and glibenclamide (5.0mg/kg body weight) was used as a standard drug.The investigational drug was administered for 28 days and the blood glucose level effect of the PHP was analysed on the 28th day after the intervention time.
 Conclusion: The experimental study showed that a persistent and substantial decrease in the average blood glucose level of diabetic rats was observed with repeated administration of PHP and glibenclamide for 28 days. PHP demonstrated substantial antidiabetic and antihyperlipidemic activity similar to the standard drug. The formulation will emerge as a possible mixture that may challenge the synthetic drug.

Antidiabetic Activity of Hydromethanolic Extract of Crude Dorstenia barnimiana Root: Validation of In Vitro and In Vivo Antidiabetic and Antidyslipidemic Activity

“…Interestingly, treatment with the extract resulted in significant improvements in lipid profile: at 4 weeks, serum TC, TGs, VLDL-C, and LDL-C had reduced and were able to enhance HDL-C levels. These findings also correlate with those of Tsofack et al 47 and Jyothsna et al, 44 who (respectively) reported that D. picta and Cinnamomum zeylanicum reduced cholesterol and TGs in experimentally induced diabetic rats. This activity of the plant extract may be related to the presence of secondary metabolites implicated in the antihyperlipidemic activity of plant extracts.…”

“…The chief source of energy in animals body is glucose, and the oral glucose-tolerance test (OGTT) enables us to evaluate the body's capacity to use glucose wisely. 44 A single dose of crude extract was administered to normoglycemic mice to verify its antihyperglycemic activity. OGTTs with 40% glucose solution resulted in hyperglycemia with a peak of 179.51 ±2.29 mg/dL 30 minutes after administration in the negative-control group, but that peak was significantly averted by both the standard drug (Glc5) and the crude extract at all test doses (P<0.001, see Table 4).…”

Novel Antidiabetic Polyherbal Formulation for Synergistic Therapeutic Effects in Streptozotocin (STZ)-Induced Diabetic Rats

“…The findings of present study are similar with the findings of others that demonstrated the substantial antihyperglycemic properties of polyherbal formulation in STZ induced diabetic rats. [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21] Our findings are similar with the findings of other authors. It has been demonstrated that our discoveries can decrease blood sugar levels in diabetic animals.…”