Evaluation of antimicrobial action and push-out bond strength and compressive strength using mineral trioxide aggregate and triple antibiotic medicament combination as root-end filling material

Abstract

Aim: The study aims to evaluate the antimicrobial properties and push-out bond and compressive strength of mineral trioxide aggregate (MTA) mixed with different ratios of triple antibiotic paste (TAP) against Enterococcus faecalis. Methodology: Antimicrobial effect was evaluated using agar well diffusion method. Materials were divided into three groups. Group 1: MTA, Group 2: MTA + TAP (2:1 ratio), and Group 3: MTA + TAP (1:1 ratio) zone of inhibition were determined after 24 h of incubation at 37°C. To evaluate push-out bond strength, 30 extracted teeth were decoronated to a length of 13 mm, followed by root canal treatment, the root resected at 3 mm from the apex, and root-end cavity was prepared and filled. Cylindrical specimens (n = 10 per group) of size 3 mm × 6 mm were prepared of MTA, MTA plus triple antibiotic 2:1 and 1:1 ratio and tested for compressive strength using Instron Universal testing machine. All the data were statistically analysed using Krusal–Wallis test (P < 0.05). Results: MTA + TAP (1:1 ratio) exhibited highest antimicrobial activity than MTA + TAP (2:1 ratio) and MTA alone. The push-out bond and compressive strength of Group 1 (MTA alone) was better compared to two groups containing MTA in combination with TAP; however, no statistical significant difference was found between the three groups. Conclusion: Combination of TAP with MTA increases the antimicrobial activity against E. faecalis without compromising much of the compressive and push-out bond strength, hence can be advocated for root-end filling.

Effect of different root canal irrigants on surface roughness and microhardness of Biodentine combined with triple antibiotic paste: An in vitro study

“…Recent literature shows that the incorporation of triple antibiotic paste (TAP) to Biodentine exhibits effective antibacterial activity against Enterococcus faecalis when utilized as root end-filling material. [ 7 ] It is reported that the incorporation of TAP with bioceramic materials such as MTA, Biodentine, and EndoSequence RRM significantly improved the calcium ion release potential compared to without TAP. [ 8 ] In addition, Biodentine combined with TAP exhibited alkaline pH and was dimensionally more stable compared to MTA with TAP.…”

“…[ 8 ] In addition, Biodentine combined with TAP exhibited alkaline pH and was dimensionally more stable compared to MTA with TAP. [ 9 ] However, the addition of any other materials to existing material affects the clinical performance of the new material. Since Biodentine is used for various endodontic applications such as pulp capping agent, an endodontic repair material for retro cavities, furcation repair, etc., It encounters different environmental conditions such as endodontic irrigants used during treatment or retreatment of root canals.…”