Aim: The study aims to evaluate the antimicrobial properties and push-out bond and compressive strength of mineral trioxide aggregate (MTA) mixed with different ratios of triple antibiotic paste (TAP) against Enterococcus faecalis. Methodology: Antimicrobial effect was evaluated using agar well diffusion method. Materials were divided into three groups. Group 1: MTA, Group 2: MTA + TAP (2:1 ratio), and Group 3: MTA + TAP (1:1 ratio) zone of inhibition were determined after 24 h of incubation at 37°C. To evaluate push-out bond strength, 30 extracted teeth were decoronated to a length of 13 mm, followed by root canal treatment, the root resected at 3 mm from the apex, and root-end cavity was prepared and filled. Cylindrical specimens (n = 10 per group) of size 3 mm × 6 mm were prepared of MTA, MTA plus triple antibiotic 2:1 and 1:1 ratio and tested for compressive strength using Instron Universal testing machine. All the data were statistically analysed using Krusal–Wallis test (P < 0.05). Results: MTA + TAP (1:1 ratio) exhibited highest antimicrobial activity than MTA + TAP (2:1 ratio) and MTA alone. The push-out bond and compressive strength of Group 1 (MTA alone) was better compared to two groups containing MTA in combination with TAP; however, no statistical significant difference was found between the three groups. Conclusion: Combination of TAP with MTA increases the antimicrobial activity against E. faecalis without compromising much of the compressive and push-out bond strength, hence can be advocated for root-end filling.

Introduction: The placement of intraorifice barriers should facilitate proper adhesion; however, it is still a challenge with existing intraorifice barrier materials. Thus, this study aims to evaluate the push-out bond strength of Glass Ionomer Cement (GIC), Tetric-N-Flow, and Biodentine as intraorifice barriers with and without diode laser activation. Materials and Methods: Human-extracted single-rooted 60 mandibular premolars were decoronated to standardize 13 mm root length. Root canal was instrumented with rotary ProTaper universal system till F4 and alternatively irrigated with 5 ml of 2.5% sodium hypochlorite and normal saline, followed by obturation using gutta-percha and AH-plus sealer. About 3.5 mm of gutta-percha was removed from the orifice using peso reamer. Horizontal tooth sections of 3.5 mm in length were obtained from the coronal third of each sample and were randomly divided into three groups (n = 20 each) for intraorifice barrier materials. Group 1: GIC; group 2: Tetric-N-Flow and group 3:Biodentine. Each group was further subdivided into two subgroups A and B (n = 10 each) with and without diode laser. After 7 days, the specimens were mounted in acrylic molds and push-out bond strength was evaluated under universal testing machine. Data analysis was done using One way-analysis of variance and post hoc Tuckey comparison tests (P ≤ 0.05). Results: Laser activation in each group showed greater push-out bond strength; however, Biodentine with laser (group 3A) exhibited higher bond strength compared to all groups (P ≤ 0.05). Conclusion: Biodentine with laser activation before placement of the coronal barrier of endodontic treated tooth increases the quality of bond strength.