Evaluation of Antimicrobial Activity of Different Aquatic Extracts Against Bacterial Isolates from UTI in Babylon Province, Iraq

Chabuck, Zainab A.; Hadi, Baraa; Hindi, Nada Khazal Kadhim

doi:10.22207/jpam.12.2.28

Cited by 4 publications

(5 citation statements)

References 26 publications

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“…However, the ethanolic extract of G. glabra contained tannins and terpenoids, table 1. [22,23,24] in which G. glabra extract showed positive inhibitory antibacterial activity against Gram-positive and Gram-negative species such as S. aureus, P. aeruginosa and E. coli Chabuck et al [25]According to our study, G. Glabra exhibited activity against tested bacterial isolates with inhibition zone ranging 18-25mm; highest inhibition was seen against E. coli (25mm) and P. aerogenosa (24 mm). In contrast, Sultana et al [26] recorded antimicrobial efficacy of the G. Glabra extract against S. aureus and E.coli , but not P. aeruginosa, while, Soulef et al [27] show that G. glabra L extract had an antibacterial effect against S. aureus and P. aeruginosa, but not the E. coli.…”

Section: -1 Chemical Analysis Of G Glabrasupporting

confidence: 68%

“…Our results revealed difference in sensitivity of bacteria to the G. glabra extract which was possibly attributed to the variation in the cell wall components, in which lipopolysaccharide(LPS) found in the outer membrane of Gram-negative bacteria is acting as a powerful permeability barrier to various compounds [34]. Extract of Licorice root includes various bioactive components and aproximalty, 40-50% of its whole dry weight components includes water-soluble bioactive compounds [25].…”

Section: -1 Chemical Analysis Of G Glabramentioning

confidence: 86%

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Chemical Analysis and Antibacterial Activity of Glycyrrhiza glabra roots

et al. 2022

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Glycyrrhiza glabra roots contain high nutritive value and substantial medicinal properties such as antibacterial, antioxidant, antimalarial, antispasmodic, anti-inflammatory and anti-hyper glycemic activities. Plant samples were collected from Babylon governorate, Iraq. Confirmed classification of G. glabra and preparation of root extract were implemented in the laboratories of college pharmacy/university of Babylon. Various extracts and chemical compounds were obtained and the antimicrobial effects of G. glabra was assessed against Staphylococcus aureus, Escherishia coli, Pseudomonas aeruginosa, and Candida albicans using agar well diffusion method and concentration range of 500-3000mg/mL. Results of the chemical analysis of G. glabra root extracts proved that the aqueous extract contained saponin, flavonoids, glycosides, but lacked alkaloids and phenols. The ethanolic extract contained tannins and terpenoids. All tested isolates were sensitive to both extracts at concentration of 3000 mg/ml. S. aureus and E. coli showed higher sensitivity, maximum effective inhibition for G. glabra aqueous extract was found against S.aureus, whereby the minimum inhibition was recorded against P. aeruginosa. Regarding G. glabra alcoholic extracts, the highest effect was noticed against P. aeruginosa, while the lowest effect was noticed on C.albicans.

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Section: -1 Chemical Analysis Of G Glabrasupporting

confidence: 68%

Section: -1 Chemical Analysis Of G Glabramentioning

confidence: 86%

Chemical Analysis and Antibacterial Activity of Glycyrrhiza glabra roots

et al. 2022

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“…Preparation of aquatic extract from fresh Viscus album and Apium graveolens was prepared by soaking 50 gm of each one as a dried powder in 100 mL distilled water and allowed to stand for about 72 hrs then sterilized by filtration (by Millipore filter paper-0.45). The above-prepared extracts were cleaned, peeled, heat dried and cut into small pieces, The electric blender was used to make the ground and placed in clean containers 16 .…”

Section: Methodsmentioning

confidence: 99%

“…Approximately 20 µL of each extract was inoculated onto wells and were made in the spread plate culture of each microbial isolate, then incubated at 37EC for 24 hrs. The examination of the diameter of the inhibitory zone by measuring it in millimetres and comparing them with the inhibitory zone of Ciprofloxacin (2 µg mLG 1 ) (the test was performed in triplicates) 16,18 .…”

Section: Antimicrobial Activity Test By Agar-well Diffusion Assay (In...mentioning

confidence: 99%

Pathogenic and Antimicrobial Properties of Aquatic Extracts of Viscus album and Apium graveolens

Oubaid¹,

Chabuck²,

Al-Saigh³

et al. 2022

Asian J. of Plant Sciences

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Background and Objective: Wild plants had been recognized to have chief roles for many populations to provide them with many requirements, also, these plants have been applied for the treatments of various complaints, especially microbial infections. This study aimed to identify the inhibitory effects of watery extracts obtained from Viscus album and Apium graveolens against the number of clinical bacterial isolates. Materials and Methods: Aqueous extracts of Viscus album and Apium graveolens were applied to examine their effects on inhibition of growth and some physiological parameters such as swarming and biofilm-formation against 19 clinical bacterial isolates (Gram-positive and negative). Method of agar wells-diffusion, tissue culture plate method for biofilm inhibition test, inhibition of adherence and swarming assay were applied for estimating and evaluating the inhibitory effects. Results: Viscus album and Apium graveolens extracts showed significant inhibitory effects against most tested Gram-positive and negative bacterial isolates with inhibitory ranges 20-35 and 18-28 mm, respectively, also with significant strong inhibition of adhesion and biofilm formation by Viscus album when compared with less inhibition by Apium graveolens. Conclusion: Aquatic extracts of Viscus album and Apium graveolens can be considered beneficial prophylaxis and treated against a wide range of pathogenic bacteria.

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“…Penelitian Karadi et.al (2011) dan Chabuck (2013) menunjukkan bahwa setiap bagian dari pisang memiliki efek yang baik untuk kesehatan tubuh, termasuk kulitnya yang selama ini terkesan tidak bermanfaat dan hanya menjadi limbah ternyata mengandung lebih banyak komponen antibiotik dan antifungal seperti alkaloid, tanin, flavonoid, saponin dansteroid dibandingkan dengan bagian tanaman pisang yang lain. 11,12,13 Penelitian Someya (2002) menyatakan bahwa kandungan zat polifenol pada kulit pisang adalah sebesar 158 mg/ 100 gr (0,158%) sedangkan pada buah pisang hanya sebesar 29,6 mg/ 100 gr (0,0296% 16 Kemudian, dilakukan uji fitokimia yang merupakan uji kalibrasi untuk melihat adanya kandungan alkaloid, tanin, flavonoid, saponin, steroid, triterpenoid, kuinon dan polifenol dalam ekstrak kulit pisang barangan. Ekstrak kental kulit pisang barangan diencerkan dengan menggunakan akuades steril hingga diperoleh konsentrasi yang diperlukan dan dihomogenkan dengan menggunakan vortex.…”

Section: Pendahuluanunclassified

UJI AKTIVITAS ANTIFUNGAL EKSTRAK KULIT PISANG BARANGAN (Musa paradisiaca L.) TERHADAP Candida albicans

Andayani¹,

Afrina²,

Sari³

2018

cakradonya dental j.

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Candida albicans (C. albicans) adalah jamur oportunistik yang pada keadaan tertentu dapat menjadi patogen di rongga mulut dan menyebakan kandidiasis oral. Penanganan kandidiasis oral umumnya menggunakan obat-obatan antifungal sintetik yang dapat menimbulkan efek samping. Kulit pisang barangan (Musa paradisiaca L.) adalah bagian yang sering dianggap tidak bermanfaat namun mengandung banyak komponen antifungal seperti alkaloid, saponin, steroid, triterpenoid, kuinon, polifenol dan flavonoid. Tujuan penelitian ini adalah untuk mengetahui aktivitas antifungal ekstrak kulit pisang barangan terhadap pertumbuhan C. albicans. Pada penelitian ini, kulit pisang barangan diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Ekstrak kulit pisang barangan yang telah diuji fitokimia, diuji aktivitas antifungalnya menggunakan metode dilusi dengan Standard Plate Count (SPC). Hasil penelitian menunjukkan bahwa ekstrak kulit Musa paradisiaca L. berpengaruh terhadap pertumbuhan C. albicans dengan konsentrasi hambat minimum (KHM) sebesar 12,5% dan Konsentrasi Bunuh Minimum sebesar 100%.Kata kunci : Candida albicans, antifungal, kulit pisang

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Evaluation of Antimicrobial Activity of Different Aquatic Extracts Against Bacterial Isolates from UTI in Babylon Province, Iraq

Cited by 4 publications

References 26 publications

Chemical Analysis and Antibacterial Activity of Glycyrrhiza glabra roots

Chemical Analysis and Antibacterial Activity of Glycyrrhiza glabra roots

Pathogenic and Antimicrobial Properties of Aquatic Extracts of Viscus album and Apium graveolens

UJI AKTIVITAS ANTIFUNGAL EKSTRAK KULIT PISANG BARANGAN (Musa paradisiaca L.) TERHADAP Candida albicans

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