Evaluation of Antimicrobial Activity of Kitaibelia vitifolia Extract against Proven Antibiotic-Susceptible and Multidrug-Resistant (MDR) Strains of Bacteria of Clinical Origin

Abstract

The goal of the present research was to screen the antimicrobial activity of an ethanolic extract of Kitaibelia vitifolia against 30 multidrug-resistant (MDR) bacterial strains isolated from healthcare-associated infections. Minimum inhibitory concentrations (MICs) of the samples against the tested bacteria were determined using the microdilution method. MDR bacterial strains were characterized using standard biochemical tests and the commercial identification systems API 20 NE and API 20 E as: Klebsiella spp. (18 isolates—I); methicillin-resistant Staphylococcus aureus (MRSA)—3; Acinetobacter spp.—3; Pseudomonas aeruginosa—5; vancomycin-resistant Enterococcus (VRE)—1. The sensitivity of isolated bacterial strains was determined using the disc diffusion method against 25 commonly used antibiotics. The highest level of sensitivity to K. vitifolia extract was confirmed in 88.89% of Klebsiella spp. isolates, E. coli ATCC 25922, two strains of MRSA (1726, 1063), Acinetobacter spp. strain 1578, and VRE strain 30, like Enterococcus faecalis ATCC 29212 (MIC =< 2.44 μg/mL). The lowest sensitivity was exhibited by 75.00% of Acinetobacter spp. (strains 1577 and 6401), where the highest values for MICs were noted (1250 μg/mL). The results indicate that the extract of K. vitifolia could be a possible source for creating new, efficient, and effective natural medicines for combat against MDR strains of bacteria.

Biologial Activity of Plant Metabolites

“…At the moment, the demand for medicinal plants for the isolation of natural BAC has increased rapidly (Cameron et al, 2005;Rai and Carpinella, 2006). Kurćubić et al (2023b) were screening the AM activity of an ethanolic extract of Kitaibelia vitifolia against 30 MDR bacterial strains isolated from healthcare-associated infections. Minimum inhibitory concentrations (MICs) of the samples against the tested bacteria were determined using the microdilution method.…”

Antibacterial and anti-biofilm profiling of Syzygium Aro-Maticum Plant Extracts against Multi-drug resistant Human Pathogens

“…Plants synthesize chemically diverse, low molecular weight bio-active substances which are used for defense mechanism and for plant's response to pathogens. Thus, it is quite indicative that these substances will be effective in in vitro conditions as well [15,16]. Aqueous, methanolic, ethanolic, and benzene extracts have significant antimicrobial efficacy and hence are promising sources of inhibitors as they've ability to modulate antibiotic activity against MDR bacteria as: efflux pump inhibitors, modification of the bacterial cell wall, inhibition of the cell division protein, inhibition of gene expression and protein synthesis [17,18].…”